Intracellular detection and evolution of site-specific proteases using a genetic selection system

Applied Biochemistry and Biotechnology

Volumn 166, Issue 5, 2012, Pages 1340-1354

  Verhoeven, Kathryn D ^a   Altstadt, Olvia C ^a   Savinov, Sergey N ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Directed evolution; Genetic reporter; Genetic selection; Proteases; Protein engineering

Indexed keywords

DIRECTED EVOLUTION; GENETIC REPORTER; GENETIC SELECTION; PROTEASES; PROTEIN ENGINEERING;

BIOCHEMICAL ENGINEERING; GENETIC ENGINEERING; PROTEINS; SUBSTRATES; VIRUSES;

ENZYME ACTIVITY;

HEXAPEPTIDE; PROTEINASE; VIRUS PROTEIN;

ARTICLE; GENETIC ANALYSIS; GENETIC SELECTION; NONHUMAN; PROTEIN DEGRADATION; TOBACCO VIRUS; VIRUS; VIRUS GENE;

BINDING SITES; CELL SURVIVAL; DIRECTED MOLECULAR EVOLUTION; ENDOPEPTIDASES; ESCHERICHIA COLI; GENES, REPORTER; INTRACELLULAR SPACE; PROTEOLYSIS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); TOBACCO ETCH VIRUS;

EID: 84858650282     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-011-9522-6     Document Type: Article

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