Regulation of life span by the gut microbiota in the short-lived african turquoise killifish

eLife

Volumn 6, Issue , 2017, Pages

  Smith, Patrick ^a   Willemsen, David ^a   Popkes, Miriam ^a   Metge, Franziska ^a   Gandiwa, Edson ^b   Reichard, Martin ^c   Valenzano, Dario Riccardo ^a,d  

^a MAX PLANCK INSTITUTE FOR BIOLOGY OF AGEING   (Germany)

^b Chinhoyi University of Technology   (Zimbabwe)

^c INSTITUTE OF VERTEBRATE BIOLOGY   (Czech Republic)

^d UNIVERSITY OF COLOGNE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; ENDONUCLEASE; METRONIDAZOLE; NEOMYCIN; VANCOMYCIN;

AGING; ARTICLE; BACTERIAL LOAD; CELL ADHESION; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; DNA EXTRACTION; ENTEROCOCCUS; ENZYME ACTIVITY; EXIGUOBACTERIUM; GENE AMPLIFICATION; GENE EDITING; GENE EXPRESSION; GENE ONTOLOGY; HALOMONAS; INTESTINE FLORA; KILLIFISH; LIFESPAN; LOCOMOTION; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PLANOCOCCUS (BACTERIUM); PREVALENCE; PROPIONIGENIUM; PSYCHROBACTER; ANIMAL; FUNDULIDAE; GASTROINTESTINAL TRACT; LONGEVITY; MICROBIOLOGY; PHYSIOLOGY;

AGING; ANIMALS; FUNDULIDAE; GASTROINTESTINAL MICROBIOME; GASTROINTESTINAL TRACT; LONGEVITY;

EID: 85030707641     PISSN: None     EISSN: 2050084X     Source Type: Journal    
DOI: 10.7554/eLife.27014     Document Type: Article

References (82)

1. Agler MT, Ruhe J, Kroll S, Morhenn C, Kim ST, Weigel D, Kemen EM. 2016. Microbial Hub Taxa Link Host and Abiotic Factors to Plant Microbiome Variation. PLOS Biology 14:e1002352. doi: 10.1371/journal.pbio.1002352
2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. Journal of Molecular Biology 215:403-410. doi: 10.1016/S0022-2836(05)80360-2
3. Asnicar F, Weingart G, Tickle TL, Huttenhower C, Segata N. 2015. Compact graphical representation of phylogenetic data and metadata with GraPhlAn. PeerJ 3:e1029. doi: 10.7717/peerj.1029
4. Baumgart DC, Carding SR. 2007. Inflammatory bowel disease: cause and immunobiology. The Lancet 369:1627-1640. doi: 10.1016/S0140-6736(07)60750-8
5. Biagi E, Franceschi C, Rampelli S, Severgnini M, Ostan R, Turroni S, Consolandi C, Quercia S, Scurti M, Monti D, Capri M, Brigidi P, Candela M. 2016. Gut Microbiota and extreme longevity. Current Biology 26:1480-1485. doi: 10.1016/j.cub.2016.04.016
6. Blažek R, Polačik M, Kačer P, Cellerino A, Řežucha R, M Syslová K, Terzibasi Tozzini E, Albrecht T, Vrtílek M, Reichard M. 2017. Repeated intraspecific divergence in life span and aging of African annual fishes along an aridity gradient. Evolution 71:386-402. doi: 10.1111/evo.13127
7. Buchon N, Broderick NA, Lemaitre B. 2013. Gut homeostasis in a microbial world: insights from Drosophila melanogaster. Nature Reviews Microbiology 11:615-626. doi: 10.1038/nrmicro3074
8. Backhed F, Fraser CM, Ringel Y, Sanders ME, Sartor RB, Sherman PM, Versalovic J, Young V, Finlay BB. 2012. Defining a healthy human gut microbiome: current concepts, future directions, and clinical applications. Cell Host & Microbe 12:611-622. doi: 10.1016/j.chom.2012.10.012
9. Cabreiro F, Gems D. 2013. Worms need microbes too: microbiota, health and aging in Caenorhabditis elegans. EMBO Molecular Medicine 5:1300-1310. doi: 10.1002/emmm.201100972
10. Caporaso JG, Bittinger K, Bushman FD, DeSantis TZ, Andersen GL, Knight R. 2010a. PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26:266-267. doi: 10.1093/bioinformatics/btp636
11. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, et al. 2010b. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7:335-336. doi: 10.1038/nmeth.f.303
12. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fraser L, Bauer M, Gormley N, Gilbert JA, Smith G, Knight R. 2012. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. The ISME Journal 6:1621-1624. doi: 10.1038/ismej.2012.8
13. Cellerino A, Valenzano DR, Reichard M. 2016. From the bush to the bench: the annual Nothobranchius fishes as a new model system in biology. Biological Reviews 91:511-533. doi: 10.1111/brv.12183
14. Cho S, Roh K, Park J, Park YS, Lee M, Cho S, Kil EJ, Cho MJ, Oh JS, Byun HS, Cho SH, Park K, Kang H, Koo J, Yeom CH, Lee S. 2017. Hydrolysis of Hyaluronic Acid in Lymphedematous Tissue Alleviates Fibrogenesis via TH1 Cell-Mediated Cytokine Expression. Scientific Reports 7:35. doi: 10.1038/s41598-017-00085-z
15. Claesson MJ, Jeffery IB, Conde S, Power SE, O’Connor EM, Cusack S, Harris HM, Coakley M, Lakshminarayanan B, O’Sullivan O, Fitzgerald GF, Deane J, O’Connor M, Harnedy N, O’Connor K, O’Mahony D, van Sinderen D, Wallace M, Brennan L, Stanton C, et al. 2012. Gut Microbiota composition correlates with diet and health in the elderly. Nature 488:178-184. doi: 10.1038/nature11319
16. Clark RI, Salazar A, Yamada R, Fitz-Gibbon S, Morselli M, Alcaraz J, Rana A, Rera M, Pellegrini M, Ja WW, Walker DW. 2015. Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality. Cell Reports 12:1656-1667. doi: 10.1016/j.celrep.2015.08.004
17. Conti B, Sanchez-Alavez M, Winsky-Sommerer R, Morale MC, Lucero J, Brownell S, Fabre V, Huitron-Resendiz S, Henriksen S, Zorrilla EP, de Lecea L, Bartfai T. 2006. Transgenic mice with a reduced core body temperature have an increased life span. Science 314:825-828. doi: 10.1126/science.1132191
18. Cox LM, Blaser MJ. 2015. Antibiotics in early life and obesity. Nature Reviews Endocrinology 11:182-190. doi: 10.1038/nrendo.2014.210
19. Csardi G, Nepusz T. 2006. The igraph software package for complex network research. Inter Journal, Complex Systems:1695.
20. DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL. 2006. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Applied and Environmental Microbiology 72:5069-5072. doi: 10.1128/AEM.03006-05
21. Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. 2013. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29:15-21. doi: 10.1093/bioinformatics/bts635
22. Dodin M, Katz DE. 2014. Faecal microbiota transplantation for Clostridium difficile infection. International Journal of Clinical Practice 68:363-368. doi: 10.1111/ijcp.12320
23. Eden E, Navon R, Steinfeld I, Lipson D, Yakhini Z. 2009. GOrilla: a tool for discovery and visualization of enriched GO terms in ranked gene lists. BMC Bioinformatics 10:48. doi: 10.1186/1471-2105-10-48
24. Edgar RC. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460-2461. doi: 10.1093/bioinformatics/btq461
25. Fontana L, Partridge L, Longo VD. 2010. Extending healthy life span-from yeast to humans. Science 328:321-326. doi: 10.1126/science.1172539
26. Friedman J, Alm EJ. 2012. Inferring correlation networks from genomic survey data. PLoS Computational Biology 8:e1002687. doi: 10.1371/journal.pcbi.1002687
27. Garrett WS. 2015. Cancer and the microbiota. Science 348:80-86. doi: 10.1126/science.aaa4972
28. Genade T, Benedetti M, Terzibasi E, Roncaglia P, Valenzano DR, Cattaneo A, Cellerino A. 2005. Annual fishes of the genus Nothobranchius as a model system for aging research. Aging Cell 4:223-233. doi: 10.1111/j.1474-9726.2005.00165.x
29. Geva-Zatorsky N, Sefik E, Kua L, Pasman L, Tan TG, Ortiz-Lopez A, Yanortsang TB, Yang L, Jupp R, Mathis D, Benoist C, Kasper DL. 2017. Mining the Human gut Microbiota for Immunomodulatory Organisms. Cell 168: e911-943. doi: 10.1016/j.cell.2017.01.022
30. Guo L, Karpac J, Tran SL, Jasper H. 2014. PGRP-SC2 Promotes Gut Immune Homeostasis to Limit Commensal Dysbiosis and Extend Lifespan. Cell 156:109-122. doi: 10.1016/j.cell.2013.12.018
31. Harel I, Benayoun BA, Machado B, Singh PP, Hu CK, Pech MF, Valenzano DR, Zhang E, Sharp SC, Artandi SE, Brunet A. 2015. A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate. Cell 160:1013-1026. doi: 10.1016/j.cell.2015.01.038
32. Harel I, Brunet A. 2015. The African Turquoise Killifish: a Model for Exploring Vertebrate Aging and Diseases in the Fast Lane. Cold Spring Harbor Symposia on Quantitative Biology 80:275-279. doi: 10.1101/sqb.2015.80.027524
33. Josenhans C, Suerbaum S. 2002. The role of motility as a virulence factor in bacteria. International Journal of Medical Microbiology 291:605-614. doi: 10.1078/1438-4221-00173
34. Kamada N, Chen GY, Inohara N, Núñez G. 2013. Control of pathogens and pathobionts by the gut microbiota. Nature Immunology 14:685-690. doi: 10.1038/ni.2608
35. Kapahi P, Chen D, Rogers AN, Katewa SD, Li PW, Thomas EL, Kockel L. 2010. With TOR, less is more: a key role for the conserved nutrient-sensing TOR pathway in aging. Cell Metabolism 11:453-465. doi: 10.1016/j.cmet.2010.05.001
36. Kenyon C, Chang J, Gensch E, Rudner A, Tabtiang R. 1993. A C. elegans mutant that lives twice as long as wild type. Nature 366:461-464. doi: 10.1038/366461a0
37. Kim Y, Nam HG, Valenzano DR. 2016. The short-lived African turquoise killifish: an emerging experimental model for ageing. Disease Models & Mechanisms 9:115-129. doi: 10.1242/dmm.023226
38. Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M, Glockner FO. 2013. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Research 41:e1. doi: 10.1093/nar/gks808
39. Kootte RS, Vrieze A, Holleman F, Dallinga-Thie GM, Zoetendal EG, de Vos WM, Groen AK, Hoekstra JB, Stroes ES, Nieuwdorp M. 2012. The therapeutic potential of manipulating gut microbiota in obesity and type 2 diabetes mellitus. Diabetes, Obesity and Metabolism 14:112-120. doi: 10.1111/j.1463-1326.2011.01483.x
40. Kostic AD, Howitt MR, Garrett WS. 2013. Exploring host-microbiota interactions in animal models and humans. Genes & Development 27:701-718. doi: 10.1101/gad.212522.112
41. Kunde S, Pham A, Bonczyk S, Crumb T, Duba M, Conrad H, Cloney D, Kugathasan S. 2013. Safety, tolerability, and clinical response after fecal transplantation in children and young adults with ulcerative colitis. Journal of Pediatric Gastroenterology and Nutrition 56:597-601. doi: 10.1097/MPG.0b013e318292fa0d
42. Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, Beiko RG, Huttenhower C. 2013. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nature Biotechnology 31:814-821. doi: 10.1038/nbt.2676
43. Langille MG, Meehan CJ, Koenig JE, Dhanani AS, Rose RA, Howlett SE, Beiko RG. 2014. Microbial shifts in the aging mouse gut. Microbiome 2:50. doi: 10.1186/s40168-014-0050-9
44. Lapierre LR, Hansen M. 2012. Lessons from C. elegans: signaling pathways for longevity. Trends in Endocrinology & Metabolism 23:637-644. doi: 10.1016/j.tem.2012.07.007
45. Lee CH, Steiner T, Petrof EO, Smieja M, Roscoe D, Nematallah A, Weese JS, Collins S, Moayyedi P, Crowther M, Ropeleski MJ, Jayaratne P, Higgins D, Li Y, Rau NV, Kim PT. 2016. Frozen vs fresh fecal microbiota transplantation and clinical resolution of diarrhea in patients with recurrent Clostridium difficile infection: a randomized clinical trial. JAMA 315:142-149. doi: 10.1001/jama.2015.18098
46. Leimbach A, Hacker J, Dobrindt U. 2013. E. coli as an all-rounder: the thin line between commensalism and pathogenicity. Current Topics in Microbiology and Immunology 358:3-32. doi: 10.1007/82_2012_303
47. Li H, Qi Y, Jasper H. 2016. Preventing Age-Related Decline of Gut Compartmentalization Limits Microbiota Dysbiosis and Extends Lifespan. Cell Host & Microbe 19:240-253. doi: 10.1016/j.chom.2016.01.008
48. Liao Y, Smyth GK, Shi W. 2014. Feature Counts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30:923-930. doi: 10.1093/bioinformatics/btt656
49. Mair W, Dillin A. 2008. Aging and survival: the genetics of life span extension by dietary restriction. Annual Review of Biochemistry 77:727-754. doi: 10.1146/annurev.biochem.77.061206.171059
50. Marotz CA, Zarrinpar A. 2016. Treating Obesity and Metabolic Syndrome with Fecal Microbiota Transplantation. The Yale Journal of Biology and Medicine 89:383-388.
51. Miquel J, Lundgren PR, Bensch KG, Atlan H. 1976. Effects of temperature on the life span, vitality and fine structure of Drosophila melanogaster. Mechanisms of Ageing and Development 5:347-370. doi: 10.1016/0047-6374(76)90034-8
52. Neis EP, Dejong CH, Rensen SS. 2015. The role of microbial amino acid metabolism in host metabolism. Nutrients 7:2930-2946. doi: 10.3390/nu7042930
53. Nezhybová V, Reichard M, Blaĕk R, Ondračková M. 2017. Metazoan parasites of African annual killifish (Nothobranchiidae): abundance, diversity, and their environmental correlates. Biotropica 49:229-238. doi: 10.1111/btp.12396
54. Nicholson JK, Holmes E, Kinross J, Burcelin R, Gibson G, Jia W, Pettersson S. 2012. Host-gut microbiota metabolic interactions. Science 336:1262-1267. doi: 10.1126/science.1223813
55. O’Toole PW, Jeffery IB. 2015. Gut microbiota and aging. Science 350:1214-1215. doi: 10.1126/science.aac8469
56. Pham LN, Kanther M, Semova I, Rawls JF. 2008. Methods for generating and colonizing gnotobiotic zebrafish. Nature Protocols 3:1862-1875. doi: 10.1038/nprot.2008.186
57. Price MN, Dehal PS, Arkin AP. 2010. Fast Tree 2-approximately maximum-likelihood trees for large alignments. PLoS One 5:e9490. doi: 10.1371/journal.pone.0009490
58. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T, Mende DR, Li J, Xu J, Li S, Li D, Cao J, Wang B, Liang H, Zheng H, Xie Y, et al. 2010. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464:59-65. doi: 10.1038/nature08821
59. Rampelli S, Candela M, Turroni S, Biagi E, Collino S, Franceschi C, O’Toole PW, Brigidi P. 2013. Functional metagenomic profiling of intestinal microbiome in extreme ageing. Aging 5:902-912. doi: 10.18632/aging.100623
60. Reichard M, Janáč M, Polačik M, Blažek R, Vrtílek M. 2017. Community assembly in Nothobranchius annual fishes: Nested patterns, environmental niche and biogeographic history. Ecology and Evolution 7:2294-2306. doi: 10.1002/ece3.2851
61. Reichwald K, Petzold A, Koch P, Downie BR, Hartmann N, Pietsch S, Baumgart M, Chalopin D, Felder M, Bens M, Sahm A, Szafranski K, Taudien S, Groth M, Arisi I, Weise A, Bhatt SS, Sharma V, Kraus JM, Schmid F, et al. 2015. Insights into Sex Chromosome Evolution and Aging from the Genome of a Short-Lived Fish. Cell 163: 1527-1538. doi: 10.1016/j.cell.2015.10.071
62. Robinson MD, McCarthy DJ, Smyth GK. 2010. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26:139-140. doi: 10.1093/bioinformatics/btp616
63. Roeselers G, Mittge EK, Stephens WZ, Parichy DM, Cavanaugh CM, Guillemin K, Rawls JF. 2011. Evidence for a core gut microbiota in the zebrafish. The ISME Journal 5:1595-1608. doi: 10.1038/ismej.2011.38
64. Sampson TR, Debelius JW, Thron T, Janssen S, Shastri GG, Ilhan ZE, Challis C, Schretter CE, Rocha S, Gradinaru V, Chesselet MF, Keshavarzian A, Shannon KM, Krajmalnik-Brown R, Wittung-Stafshede P, Knight R, Mazmanian SK. 2016. Gut Microbiota Regulate Motor Deficits and Neuroinflammation in a Model of Parkinson’s Disease. Cell 167:1469-1480. doi: 10.1016/j.cell.2016.11.018
65. Schuijt TJ, Lankelma JM, Scicluna BP, de Sousa e Melo F, Roelofs JJ, de Boer JD, Hoogendijk AJ, de Beer R, de Vos A, Belzer C, de Vos WM, van der Poll T, Wiersinga WJ. 2016. The gut microbiota plays a protective role in the host defence against pneumococcal pneumonia. Gut 65:575-583. doi: 10.1136/gutjnl-2015-309728
66. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. 2011. Metagenomic biomarker discovery and explanation. Genome Biology 12:R60. doi: 10.1186/gb-2011-12-6-r60
67. Semova I, Carten JD, Stombaugh J, Mackey LC, Knight R, Farber SA, Rawls JF. 2012. Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. Cell Host & Microbe 12:277-288. doi: 10.1016/j.chom.2012.08.003
68. Sokol H, Pigneur B, Watterlot L, Lakhdari O, Bermúdez-Humarán LG, Gratadoux JJ, Blugeon S, Bridonneau C, Furet JP, Corthier G, Grangette C, Vasquez N, Pochart P, Trugnan G, Thomas G, Blottière HM, Doré J, Marteau P, Seksik P, Langella P. 2008. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. PNAS 105:16731-16736. doi: 10.1073/pnas.0804812105
69. Sommer F, Backhed F. 2013. The gut microbiota-masters of host development and physiology. Nature Reviews Microbiology 11:227-238. doi: 10.1038/nrmicro2974
70. Steffen KK, MacKay VL, Kerr EO, Tsuchiya M, Hu D, Fox LA, Dang N, Johnston ED, Oakes JA, Tchao BN, Pak DN, Fields S, Kennedy BK, Kaeberlein M. 2008. Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4. Cell 133:292-302. doi: 10.1016/j.cell.2008.02.037
71. Stephens WZ, Burns AR, Stagaman K, Wong S, Rawls JF, Guillemin K, Bohannan BJ. 2016. The composition of the zebrafish intestinal microbial community varies across development. The ISME Journal 10:644-654. doi: 10.1038/ismej.2015.140
72. Tian X, Azpurua J, Hine C, Vaidya A, Myakishev-Rempel M, Ablaeva J, Mao Z, Nevo E, Gorbunova V, Seluanov A. 2013. High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole rat. Nature 499:346-349. doi: 10.1038/nature12234
73. Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI. 2006. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444:1027-1131. doi: 10.1038/nature05414
74. Valenzano DR, Terzibasi E, Cattaneo A, Domenici L, Cellerino A. 2006a. Temperature affects longevity and age-related locomotor and cognitive decay in the short-lived fish Nothobranchius furzeri. Aging Cell 5:275-278. doi: 10.1111/j.1474-9726.2006.00212.x
75. Valenzano DR, Terzibasi E, Genade T, Cattaneo A, Domenici L, Cellerino A. 2006b. Resveratrol prolongs lifespan and retards the onset of age-related markers in a short-lived vertebrate. Current Biology 16:296-300. doi: 10.1016/j.cub.2005.12.038
76. Valenzano DR, Sharp S, Brunet A. 2011. Transposon-Mediated Transgenesis in the Short-Lived African Killifish Nothobranchius furzeri, a Vertebrate Model for Aging. G3; Genes|Genomes|Genetics 1:531-538. doi: 10.1534/g3.111.001271
77. Valenzano DR, Benayoun BA, Singh PP, Zhang E, Etter PD, Hu CK, Clément-Ziza M, Willemsen D, Cui R, Harel I, Machado BE, Yee MC, Sharp SC, Bustamante CD, Beyer A, Johnson EA, Brunet A. 2015. The African Turquoise Killifish Genome Provides Insights into Evolution and Genetic Architecture of Lifespan. Cell 163:1539-1554. doi: 10.1016/j.cell.2015.11.008
78. Valenzano DR. 2017. publications_Smith_2017_Figure7E. cd08cca. Github. https://github.com/dvalenzano/ publications/blob/master/Smith_2017_Figure7E.ipynb
79. Van Voorhies WA, Ward S. 1999. Genetic and environmental conditions that increase longevity in Caenorhabditis elegans decrease metabolic rate. PNAS 96:11399-11403. doi: 10.1073/pnas.96.20.11399
80. Xu MQ, Cao HL, Wang WQ, Wang S, Cao XC, Yan F, Wang BM. 2015. Fecal microbiota transplantation broadening its application beyond intestinal disorders. World Journal of Gastroenterology 21:102-111. doi: 10.3748/wjg.v21.i1.102
81. Zhao Y, Zhao L, Zheng X, Fu T, Guo H, Ren F. 2013. Lactobacillus salivarius strain FDB89 induced longevity in Caenorhabditis elegans by dietary restriction. Journal of Microbiology 51:183-188. doi: 10.1007/s12275-013-2076-2
82. Zoetendal EG, Vaughan EE, de Vos WM. 2006. A microbial world within Us. Molecular Microbiology 59:1639-1650. doi: 10.1111/j.1365-2958.2006.05056.x