Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex

Cell

Volumn 171, Issue 2, 2017, Pages 414-426.e12

  Guo, Tai Wei ^a   Bartesaghi, Alberto ^a   Yang, Hui ^b   Falconieri, Veronica ^a   Rao, Prashant ^a   Merk, Alan ^a   Eng, Edward T ^c   Raczkowski, Ashleigh M ^c   Fox, Tara ^a,d   Earl, Lesley A ^a   Patel, Dinshaw J ^b   Subramaniam, Sriram ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c NEW YORK STRUCTURAL BIOLOGY CENTER   (United States)

^d FREDERICK NATIONAL LABORATORY FOR CANCER RESEARCH   (United States)

Author keywords

adaptive immunity; anti CRISPR inhibitors; conformational transitions; CRISPR Cas Csy complexes; CRISPR Cas systems; cryoelectron microscopy; DNA protein interactions; gene editing; PAM recognition

Indexed keywords

ACRF1 POLYPEPTIDE; ACRF10 POLYPEPTIDE; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; DOUBLE STRANDED DNA; HELICASE; NUCLEASE; POLYPEPTIDE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; MULTIPROTEIN COMPLEX; VIRUS DNA;

ARTICLE; CELLULAR IMMUNITY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONFORMATIONAL TRANSITION; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; DNA BINDING; DNA DEGRADATION; DNA DETERMINATION; DNA PROTEIN COMPLEX; DNA SEQUENCE; GENE TARGETING; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROKARYOTIC CELL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; RNA DEGRADATION; RNA INTERFERENCE; BACTERIOPHAGE; CHEMICAL MODEL; CHEMISTRY; CRISPR CAS SYSTEM; GENETICS; IMMUNOLOGY; METABOLISM; MOLECULAR MODEL; PSEUDOMONAS AERUGINOSA; ULTRASTRUCTURE;

BACTERIAL PROTEINS; BACTERIOPHAGES; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; CRYOELECTRON MICROSCOPY; DNA, VIRAL; MODELS, CHEMICAL; MODELS, MOLECULAR; MULTIPROTEIN COMPLEXES; PSEUDOMONAS AERUGINOSA;

EID: 85030552464     PISSN: 00928674     EISSN: 10974172     Source Type: Journal    
DOI: 10.1016/j.cell.2017.09.006     Document Type: Article

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