Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins

Nature

Volumn 526, Issue 7571, 2015, Pages 136-139

  Bondy Denomy, Joseph ^a,c   Garcia, Bianca ^a   Strum, Scott ^a   Du, Mingjian ^a   Rollins, Maryclare F ^b   Hidalgo Reyes, Yurima ^a   Wiedenheft, Blake ^b   Maxwell, Karen L ^a   Davidson, Alan R ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b MONTANA STATE UNIVERSITY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CAS3 PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; SINGLE STRANDED DNA; UNCLASSIFIED DRUG; CRISPR ASSOCIATED PROTEIN; DNA BINDING PROTEIN; DNA HELICASE; ENDONUCLEASE; PROTEIN BINDING; PROTEIN SUBUNIT; REPRESSOR PROTEIN; VIRUS DNA; VIRUS PROTEIN;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; IMMUNE SYSTEM; INHIBITION; PROTEIN; SURVIVAL; VIRUS;

ADAPTIVE IMMUNITY; ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; ENZYME ACTIVITY; PRIORITY JOURNAL; PROTEIN DNA BINDING; STOICHIOMETRY; ANTAGONISTS AND INHIBITORS; BACTERIOPHAGE; BACTERIUM; ENZYME SPECIFICITY; GENETICS; METABOLISM; MOLECULAR EVOLUTION; PHYSIOLOGY; PROTEIN SUBUNIT; VIROLOGY;

BACTERIA (MICROORGANISMS);

BACTERIA; BACTERIOPHAGES; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DNA HELICASES; DNA, VIRAL; DNA-BINDING PROTEINS; ENDONUCLEASES; EVOLUTION, MOLECULAR; PROTEIN BINDING; PROTEIN SUBUNITS; REPRESSOR PROTEINS; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

EID: 84943188033     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature15254     Document Type: Article

References (21)

1. Barrangou, R. et al. CRISPR provides acquired resistance against viruses in prokaryotes. Science 315, 1709-1712 (2007).
2. Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nature Rev. Microbiol. 9, 467-477 (2011).
3. Jore, M. M., Brouns, S. J. J. & van der Oost, J. RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements. Cold Spring Harb. Perspect. Biol. 4, a003657 (2012).
4. Bondy-Denomy, J., Pawluk, A.,Maxwell, K. L.&Davidson, A. R.Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature 493, 429-432 (2013).
5. van der Oost, J., Westra, E. R., Jackson, R. N. & Wiedenheft, B. Unravelling the structural and mechanistic basis of CRISPR-Cas systems. Nature Rev. Microbiol. 12, 479-492 (2014).
6. Haurwitz, R. E., Jinek, M., Wiedenheft, B., Zhou, K. & Doudna, J. A. Sequence-and structure-specific RNA processing by a CRISPR endonuclease. Science 329, 1355-1358 (2010).
7. Wiedenheft, B. et al. RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proc. Natl Acad. Sci. USA 108, 10092-10097 (2011).
8. van Duijn, E. et al. Native tandem and ionmobilitymass spectrometry highlight structural and modular similarities in clustered-regularly-interspaced shotpalindromic-repeats (CRISPR)-associated protein complexes from Escherichia coli and Pseudomonas aeruginosa. Mol. Cell. Proteomics 11, 1430-1441 (2012).
9. Westra, E. R. et al. CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invaderDNAby Cascade and Cas3. Mol. Cell 46, 595-605 (2012).
10. Huo, Y. et al. Structures of CRISPR Cas3 offer mechanistic insights into Cascadeactivated DNA unwinding and degradation. Nature Struct. Mol. Biol. 21, 771-777 (2014).
11. Rollins, M. F., Schuman, J. T., Paulus, K., Bukhari, H. S. T. & Wiedenheft, B. Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa. Nucleic Acids Res. 43, 2216-2222 (2015).
12. Semenova, E. et al. Interference by clustered regularly interspaced short palindromicrepeat (CRISPR)RNAis governedby a seed sequence. Proc.NatlAcad. Sci. USA 108, 10098-10103 (2011).
13. Wurtzel, O. et al. The single-nucleotide resolution transcriptome of Pseudomonas aeruginosa grown in body temperature. PLoS Pathog. 8, e1002945 (2012).
14. Luo,M. L., Mullis, A. S., Leenay, R. T. & Beisel, C. L. Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression. Nucleic Acids Res. 43, 674-681 (2015).
15. Rath, D., Amlinger, L., Hoekzema, M., Devulapally, P. R. & Lundgren, M. Efficient programmable gene silencing by Cascade. Nucleic Acids Res. 43, 237-246 (2015).
16. Cady, K. C. & O'Toole, G. A. Non-identity-mediated CRISPR-bacteriophage interaction mediated via the Csy and Cas3 proteins. J. Bacteriol. 193, 3433-3445 (2011).
17. Jackson, R. N. et al. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science 345, 1473-1479 (2014).
18. Mulepati, S., Héroux, A. & Bailey, S. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science 345, 1479-1484 (2014).
19. Pawluk, A., Bondy-Denomy, J., Cheung, V. H. W., Maxwell, K. L. & Davidson, A. R. A new group of phage anti-CRISPR genes inhibits the type I-E CRISPR-Cas system of Pseudomonas aeruginosa. MBio 5, e00896 (2014).
20. Westra, E. R., Buckling, A. & Fineran, P. C. CRISPR-Cas systems: beyond adaptive immunity. Nature Rev. Microbiol. 12, 317-326 (2014).
21. Cady, K. C., Bondy-Denomy, J., Heussler, G. E., Davidson, A. R. & O'Toole, G. A. The CRISPR/Cas adaptive immune system of Pseudomonas aeruginosa mediates resistance to naturally occurring and engineered phages. J. Bacteriol. 194, 5728-5738 (2012).