Co-expression of an alcohol dehydrogenase and a cyclohexanone monooxygenase for cascade reactions facilitates the regeneration of the NADPH cofactor

Enzyme and Microbial Technology

Volumn 108, Issue , 2018, Pages 53-58

  Kohl, Anna ^a   Srinivasamurthy, Vishnu ^a   Böttcher, Dominique ^a   Kabisch, Johannes ^b   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Co expression; Cyclohexanone monooxygenase; Protein engineering; Ribosome binding site; Whole cell biocatalysis

Indexed keywords

BIOCHEMICAL ENGINEERING; CATALYSIS; CELL ENGINEERING; ESCHERICHIA COLI; MACROMOLECULES;

CO-EXPRESSION; CYCLOHEXANONE MONOOXYGENASE; PROTEIN ENGINEERING; RIBOSOME BINDING SITES; WHOLE-CELL BIOCATALYSIS;

ENZYMES;

ALCOHOL DEHYDROGENASE; CYCLOHEXANONE MONOOXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; BACTERIAL PROTEIN; CYCLOHEXANOL DERIVATIVE; CYCLOHEXANONE OXYGENASE; FUNGAL PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXYGENASE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; BACTERIAL CELL; BINDING SITE; BIOCATALYSIS; CELL ENGINEERING; CELL SUSPENSION; CONTROLLED STUDY; ENZYME ENGINEERING; ENZYME SUBSTRATE; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; RIBOSOME; WHOLE CELL BIOCATALYSIS; ACINETOBACTER CALCOACETICUS; CANDIDA; ENZYMOLOGY; GENE VECTOR; GENETICS; LACTOBACILLUS; METABOLISM; PROCEDURES; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

ACINETOBACTER CALCOACETICUS; ALCOHOL DEHYDROGENASE; BACTERIAL PROTEINS; BIOCATALYSIS; CANDIDA; CYCLOHEXANOLS; ESCHERICHIA COLI; FUNGAL PROTEINS; GENETIC VECTORS; LACTOBACILLUS; LIPASE; MUTAGENESIS, SITE-DIRECTED; NADP; OXYGENASES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 85029476109     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2017.09.003     Document Type: Article

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