FastCloning: A highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Li, Chaokun ^a   Wen, Aiyun ^a   Shen, Benchang ^a,c   Lu, Jia ^b   Huang, Yao ^b   Chang, Yongchang ^a  

^a BARROW NEUROLOGICAL INSTITUTE   (United States)

^b ST JOSEPH'S HOSPITAL AND MEDICAL CENTER   (United States)

^c GUANGZHOU MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DESIRED POSITION; DNA FRAGMENT; DNA POLYMERASE; DNA-TEMPLATE; E. COLI; HIGH FIDELITY; MOLECULAR CLONING; MULTIPLE MUTATIONS; PCR CLONING; PCR PRODUCTS; PCR REACTIONS; PLASMID VECTORS; RANDOM MUTATION; VERSATILE TOOLS;

CLONE CELLS; DNA; DNA SEQUENCES; ESCHERICHIA COLI; GENES; POLYMERASE CHAIN REACTION; PURIFICATION; VECTORS;

CLONING;

COMPLEMENTARY DNA; DNA POLYMERASE; PLASMID VECTOR; DNA DIRECTED DNA POLYMERASE; HYBRID PROTEIN; PRIMER DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; BACTERIAL CELL; DNA FRAGMENTATION; DNA SEQUENCE; DNA TEMPLATE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE INSERTION; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; ANIMAL; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; CHEMISTRY; GENE VECTOR; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; OOCYTE; XENOPUS LAEVIS;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA RESTRICTION ENZYMES; DNA, COMPLEMENTARY; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OOCYTES; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; TRANSFORMATION, BACTERIAL; XENOPUS LAEVIS;

EID: 80053929147     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-92     Document Type: Article

References (9)

1. Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 2001, Cold Spring Harbor Laboratory Press, New York.
2. Marchuk D, Drumm M, Saulino A, Collins F. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Research 1991, 19:1154. 10.1093/nar/19.5.1154, 333800, 2020552.
3. Aslanidis C, de Jong P. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Research 1990, 18:6069-6074. 10.1093/nar/18.20.6069, 332407, 2235490.
4. Haun R, Serventi I, Moss J. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 1992, 13:515-518.
5. Walhout A, Temple G, Brasch M, Hartley J, Lorson M, van den Heuvel S, Vidal M. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods in Enzymology 2000, 328:575-592.
6. Zuo P, Rabie B. One-step DNA fragment assembly and circularization for gene cloning. Current Issues in Molecular Biology 2009, 12:11-16.
7. Bryksin A, Matsumura I. Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. BioTechniques 2010, 48:463-465. 10.2144/000113418, 3121328, 20569222.
8. Yang J, Zhang Z, Zhang X, Luo Q. A ligation-independent cloning method using nicking DNA endonuclease. BioTechniques 2010, 49:817-821. 10.2144/000113520, 21091446.
9. Liman E, Tytgat J, Hess P. Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs. Neuron 1992, 9:861-871. 10.1016/0896-6273(92)90239-A, 1419000.