Engineering a minimal G protein to facilitate crystallisation of G protein-coupled receptors in their active conformation

Protein Engineering, Design and Selection

Volumn 29, Issue 12, 2016, Pages 583-593

  Carpenter, Byron ^a   Tate, Christopher G ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Complex; G protein; G protein coupled receptor; G s; GPCR; Mini G protein; Mini G s

Indexed keywords

SIGNAL TRANSDUCTION;

COMPLEX; G PROTEIN; G PROTEIN COUPLED RECEPTORS; GPCR; MINI-GS;

PROTEINS;

ADENYLATE CYCLASE; BETA 1 ADRENERGIC RECEPTOR; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; ISOPRENALINE; ADENOSINE A2A RECEPTOR; PEPTIDE FRAGMENT;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; COMPETITIVE BINDING ASSAY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; MOLECULAR WEIGHT; MUTAGENESIS; MUTATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RECEPTOR BINDING; THERMOSTABILITY; BINDING SITE; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR MODEL; PROTEIN DOMAIN; PROTEIN STABILITY; TEMPERATURE;

BINDING SITES; CRYSTALLIZATION; GTP-BINDING PROTEINS; MODELS, MOLECULAR; MUTAGENESIS; PEPTIDE FRAGMENTS; PROTEIN DOMAINS; PROTEIN ENGINEERING; PROTEIN STABILITY; RECEPTOR, ADENOSINE A2A; TEMPERATURE;

EID: 85018328852     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzw049     Document Type: Article

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