Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells

Journal of Biotechnology

Volumn 247, Issue , 2017, Pages 42-49

  Lin, Lin ^a   Petersen, Trine Skov ^a   Jensen, Kristopher Torp ^a,b   Bolund, Lars ^a   Kühn, Ralf ^c,d   Luo, Yonglun ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^d BERLIN INSTITUTE OF HEALTH   (Germany)

Author keywords

CRISPR; DSB; Rec A; SpCas9; SpCas9(1.1); SSA

Indexed keywords

CELLS; CYTOLOGY; ESCHERICHIA COLI; GENES; MAMMALS; PROTEINS; REPAIR;

CRISPR; DOUBLE-STRAND DNAS; EUKARYOTIC CELLS; REC A; SPCAS9; SPCAS9(1.1); STRAND ANNEALING; STREPTOCOCCUS PYOGENES;

RECOMBINANT PROTEINS;

BACTERIAL PROTEIN; CASPASE 9; RECA PROTEIN; RECOMBINANT PROTEIN; STREPTOCOCCUS PYOGENES CAS9 PROTEIN; UNCLASSIFIED DRUG; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; ENDONUCLEASE; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DOUBLE STRANDED DNA BREAK; EMBRYO; ESCHERICHIA COLI; EUKARYOTIC CELL; GENE CONVERSION; GENE DELETION; GENE FUSION; GENE INACTIVATION; HEK293 CELL LINE; HUMAN; HUMAN CELL; INDEL MUTATION; NONHUMAN; PRIORITY JOURNAL; RECA GENE; STREPTOCOCCUS PYOGENES; ANIMAL; DNA REPAIR; GENETICS; HELA CELL LINE; MAMMAL; METABOLISM; PROCEDURES; TUMOR CELL LINE;

ANIMALS; BACTERIAL PROTEINS; CELL LINE, TUMOR; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; ENDONUCLEASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE KNOCKOUT TECHNIQUES; HEK293 CELLS; HELA CELLS; HUMANS; INDEL MUTATION; MAMMALS; REC A RECOMBINASES; RECOMBINANT FUSION PROTEINS; STREPTOCOCCUS PYOGENES;

EID: 85014605483     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2017.02.024     Document Type: Article

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