High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: Developing a pig model for breast cancer

Transgenic Research

Volumn 20, Issue 5, 2011, Pages 975-988

  Luo, Yonglun ^a,c   Li, Juan ^b   Liu, Ying ^b   Lin, Lin ^b,c   Du, Yutao ^c   Li, Shengting ^a   Yang, Huanming ^c   Vajta, Gábor ^b   Callesen, Henrik ^b   Bolund, Lars ^a,c   Sørensen, Charlotte Brandt ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b AARHUS UNIVERSITY   (Denmark)

^c BGI SHENZHEN   (China)

Author keywords

BRCA1; Breast cancer; Gene targeting; Minipig; rAAV

Indexed keywords

BRCA1 PROTEIN;

ADENO ASSOCIATED VIRUS; ANIMAL; ARTICLE; BREAST TUMOR; DISEASE MODEL; EMBRYO TRANSFER; FEMALE; GENE INACTIVATION; GENE TARGETING; GENETICS; HETEROZYGOTE; METABOLISM; MINIPIG; PHYLOGENY; SWINE;

ANIMALS; BRCA1 PROTEIN; BREAST NEOPLASMS; DEPENDOVIRUS; DISEASE MODELS, ANIMAL; EMBRYO TRANSFER; FEMALE; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; HETEROZYGOTE; PHYLOGENY; SWINE; SWINE, MINIATURE;

ADENO-ASSOCIATED VIRUS; SUIDAE;

EID: 80052849451     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-010-9472-8     Document Type: Article

References (34)

1. Alberg AJ, Lam AP, Helzlsouer KJ (1999) Epidemiology, prevention, and early detection of breast cancer. Curr Opin Oncol 11: 435-441.
2. Bennett LM, McAllister KA, Blackshear PE, Malphurs J, Goulding G, Collins NK, Ward T, Bunch DO, Eddy EM, Davis BJ, Wiseman RW (2000) BRCA2-null embryonic survival is prolonged on the BALB/c genetic background. Mol Carcinog 28: 174-183.
3. Bouwman P, Jonkers J (2008) Mouse models for BRCA1 associated tumorigenesis: from fundamental insights to preclinical utility. Cell Cycle 7: 2647-2653.
4. Boyd M, Harris F, McFarlane R, Davidson HR, Black DM (1995) A human BRCA1 gene knockout. Nature 375: 541-542.
5. Campbell KH, Alberio R, Choi I, Fisher P, Kelly RD, Lee JH, Maalouf W (2005) Cloning: eight years after Dolly. Reprod Domest Anim 40: 256-268.
6. Crook T, Crossland S, Crompton MR, Osin P, Gusterson BA (1997) p53 mutations in BRCA1-associated familial breast cancer. Lancet 350: 638-639.
7. Deng CX (2006) BRCA1: cell cycle checkpoint, genetic instability, DNA damage response and cancer evolution. Nucleic Acids Res 34: 1416-1426.
8. Du Y, Kragh PM, Zhang Y, Li J, Schmidt M, Bogh IB, Zhang X, Purup S, Jorgensen AL, Pedersen AM, Villemoes K, Yang H, Bolund L, Vajta G (2007) Piglets born from handmade cloning, an innovative cloning method without micromanipulation. Theriogenology 68: 1104-1110.
9. Gowen LC, Johnson BL, Latour AM, Sulik KK, Koller BH (1996) Brca1 deficiency results in early embryonic lethality characterized by neuroepithelial abnormalities. Nat Genet 12: 191-194.
10. Grieger JC, Choi VW, Samulski RJ (2006) Production and characterization of adeno-associated viral vectors. Nat Protoc 1: 1412-1428.
11. Hakem R, de la Pompa JL, Sirard C, Mo R, Woo M, Hakem A, Wakeham A, Potter J, Reitmair A, Billia F, Firpo E, Hui CC, Roberts J, Rossant J, Mak TW (1996) The tumor suppressor gene Brca1 is required for embryonic cellular proliferation in the mouse. Cell 85: 1009-1023.
12. Jeng YM, Cai-Ng S, Li A, Furuta S, Chew H, Chen PL, Lee EY, Lee WH (2007) Brca1 heterozygous mice have shortened life span and are prone to ovarian tumorigenesis with haploinsufficiency upon ionizing irradiation. Oncogene 26: 6160-6166.
13. Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B (2004) Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses. Nucleic Acids Res 32: e3.
14. Kragh PM, Nielsen AL, Li J, Du Y, Lin L, Schmidt M, Bogh IB, Holm IE, Jakobsen JE, Johansen MG, Purup S, Bolund L, Vajta G, Jorgensen AL (2009) Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw. Transgenic Res 18: 545-558.
15. Lane TF, Deng C, Elson A, Lyu MS, Kozak CA, Leder P (1995) Expression of Brca1 is associated with terminal differentiation of ectodermally and mesodermally derived tissues in mice. Genes Dev 9: 2712-2722.
16. Liu X, Holstege H, van der Gulden H, Treur-Mulder M, Zevenhoven J, Velds A, Kerkhoven RM, van Vliet MH, Wessels LF, Peterse JL, Berns A, Jonkers J (2007) Somatic loss of BRCA1 and p53 in mice induces mammary tumors with features of human BRCA1-mutated basal-like breast cancer. Proc Natl Acad Sci USA 104: 12111-12116.
17. Ludwig T, Chapman DL, Papaioannou VE, Efstratiadis A (1997) Targeted mutations of breast cancer susceptibility gene homologs in mice: lethal phenotypes of Brca1, Brca2, Brca1/Brca2, Brca1/p53, and Brca2/p53 nullizygous embryos. Genes Dev 11: 1226-1241.
18. Ludwig T, Fisher P, Ganesan S, Efstratiadis A (2001) Tumorigenesis in mice carrying a truncating Brca1 mutation. Genes Dev 15: 1188-1193.
19. McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE, Kind AJ (2000) Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405: 1066-1069.
20. Miki Y, Swensen J, Shattuck-Eidens D, Futreal PA, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett LM, Ding W et al (1994) A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science 266: 66-71.
21. Rice JC, Ozcelik H, Maxeiner P, Andrulis I, Futscher BW (2000) Methylation of the BRCA1 promoter is associated with decreased BRCA1 mRNA levels in clinical breast cancer specimens. Carcinogenesis 21: 1761-1765.
22. Rogers CS, Hao Y, Rokhlina T, Samuel M, Stoltz DA, Li Y, Petroff E, Vermeer DW, Kabel AC, Yan Z, Spate L, Wax D, Murphy CN, Rieke A, Whitworth K, Linville ML, Korte SW, Engelhardt JF, Welsh MJ, Prather RS (2008) Production of CFTR-null and CFTR-DeltaF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer. J Clin Invest 118: 1571-1577.
23. Rosen EM, Fan S, Pestell RG, Goldberg ID (2003) BRCA1 gene in breast cancer. J Cell Physiol 196: 19-41.
24. Russell DW, Hirata RK (1998) Human gene targeting by viral vectors. Nat Genet 18: 325-330.
25. Schmidt M, Kragh PM, Li J, Du Y, Lin L, Liu Y, Bogh IB, Winther KD, Vajta G and Callesen H (2010) Pregnancies and piglets from large white sow recipients after two transfer methods of cloned and transgenic embryos of different pig breeds. Theriogenology 74: 1233-1240.
26. Szabo CI, Worley T, Monteiro AN (2004) Understanding germ-line mutations in BRCA1. Cancer Biol Ther 3: 515-520.
27. Tian XC, Park J, Bruno R, French R, Jiang L, Prather RS (2009) Altered gene expression in cloned piglets. Reprod Fertil Dev 21: 60-66.
28. Vajta G (2007) Handmade cloning: the future way of nuclear transfer? Trends Biotechnol 25: 250-253.
29. Vajta G, Peura TT, Holm P, Paldi A, Greve T, Trounson AO, Callesen H (2000) New method for culture of zona-included or zona-free embryos: the well of the well (WOW) system. Mol Reprod Dev 55: 256-264.
30. Venkitaraman AR (2002) Cancer susceptibility and the functions of BRCA1 and BRCA2. Cell 108: 171-182.
31. Wernersson R, Schierup MH, Jorgensen FG, Gorodkin J, Panitz F, Staerfeldt HH, Christensen OF, Mailund T, Hornshoj H, Klein A, Wang J, Liu B, Hu S, Dong W, Li W, Wong GK, Yu J, Bendixen C, Fredholm M, Brunak S, Yang H, Bolund L (2005) Pigs in sequence space: a 0. 66X coverage pig genome survey based on shotgun sequencing. BMC Genomics 6: 70.
32. Yoshida K, Miki Y (2004) Role of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damage. Cancer Sci 95: 866-871.
33. Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S (2002) Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod 66: 112-119.
34. Young LE, Sinclair KD, Wilmut I (1998) Large offspring syndrome in cattle and sheep. Rev Reprod 3: 155-163.