Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

Cell Systems

Volumn 4, Issue 3, 2017, Pages 291-305.e7

  Koo, Byoung Mo ^a   Kritikos, George ^d   Farelli, Jeremiah D ^e   Todor, Horia ^a   Tong, Kenneth ^a   Kimsey, Harvey ^f   Wapinski, Ilan ^f   Galardini, Marco ^g   Cabal, Angelo ^a   Peters, Jason M ^a   Hachmann, Anna Barbara ^f   Rudner, David Z ^f   Allen, Karen N ^e   Typas, Athanasios ^d   Gross, Carol A ^a,b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^e Boston University   (United States)

^f HARVARD MEDICAL SCHOOL   (United States)

^g WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

auxotrophic gene; Bacillus subtilis; competence; essential gene; Genome wide screening; growth; high throughput methods for transformation and double mutant analysis; ordered gene deletion mutant library; sporulation; ysaA

Indexed keywords

AMINO ACID; CARBON; NITROGEN;

ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; ESSENTIAL GENE; GENE LIBRARY; LOW TEMPERATURE; NONHUMAN; PRIORITY JOURNAL; BACTERIAL SPORE; GENE DELETION; GENETICS; GENOMICS; HIGH THROUGHPUT SCREENING; PROCEDURES;

AMINO ACIDS; BACILLUS SUBTILIS; GENE DELETION; GENE LIBRARY; GENOMIC LIBRARY; GENOMICS; HIGH-THROUGHPUT SCREENING ASSAYS; SEQUENCE DELETION; SPORES, BACTERIAL;

EID: 85011856675     PISSN: 24054712     EISSN: 24054720     Source Type: Journal    
DOI: 10.1016/j.cels.2016.12.013     Document Type: Article

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