Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

mBio

Volumn 6, Issue 3, 2015, Pages 1-15

  Wetmore, Kelly M ^a   Price, Morgan N ^a   Waters, Robert J ^b   Lamson, Jacob S ^a   He, Jennifer ^a   Hoover, Cindi A ^b   Blow, Matthew J ^b   Bristow, James ^b   Butland, Gareth ^a   Arkin, Adam P ^a,c   Deutschbauer, Adam ^a  

^a LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^b DOE JOINT GENOME INSTITUTE   (United States)

^c University of California at Berkeley   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; DEXTRO MANNOSE ISOMERASE; ISOMERASE; MANNITOL; UNCLASSIFIED DRUG; TRANSPOSON;

ANALYTIC METHOD; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIUM MUTANT; CARBOHYDRATE METABOLISM; CARBON SOURCE; CARBON UTILIZATION; CATABOLISM; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DNA BARCODING; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC ANALYZER; MARINER GENE; MICROBIAL DIVERSITY; NONHUMAN; PHAEOBACTER INHIBENS; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS STUTZERI; RANDOM BAR CODE TRANSPOSON SITE SEQUENCING; REPRODUCIBILITY; SHEWANELLA; SHEWANELLA AMAZONENSIS; SHEWANELLA ONEIDENSIS; TRANSPOSON; CHROMOSOMAL MAPPING; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; HIGH THROUGHPUT SEQUENCING; MUTATION; NUCLEOTIDE SEQUENCE; PSEUDOMONAS; REPRODUCTIVE FITNESS; RHODOBACTERACEAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PHAEOBACTER INHIBENS; PSEUDOMONAS STUTZERI; SHEWANELLA AMAZONENSIS; SHEWANELLA ONEIDENSIS;

BASE SEQUENCE; CHROMOSOME MAPPING; DNA BARCODING, TAXONOMIC; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENE LIBRARY; GENETIC FITNESS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MUTAGENESIS, INSERTIONAL; MUTATION; PHENOTYPE; PSEUDOMONAS; REPRODUCIBILITY OF RESULTS; RHODOBACTERACEAE; SHEWANELLA;

EID: 84936929916     PISSN: 21612129     EISSN: 21507511     Source Type: Journal    
DOI: 10.1128/mBio.00306-15     Document Type: Article

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