The T296V Mutant of Amorpha-4,11-diene Synthase Is Defective in Allylic Diphosphate Isomerization but Retains the Ability To Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene

Biochemistry

Volumn 55, Issue 48, 2016, Pages 6599-6604

  Li, Zhenqiu ^a   Gao, Ruiping ^a   Hao, Qinggang ^a   Zhao, Huifang ^a   Cheng, Longbin ^a   He, Fang ^a   Liu, Li ^a   Liu, Xiuhua ^a   Chou, Wayne K W ^b   Zhu, Huajie ^a   Cane, David E ^b  

^a HEBEI UNIVERSITY   (China)

^b BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

ALLYLIC REARRANGEMENT; AMINO ACID RESIDUES; AMORPHA-4 ,11-DIENE; CYCLASE ACTIVITY; FARNESYL DIPHOSPHATE; IN-VITRO ASSAYS; NATURAL SUBSTRATES; NOCV1; SESQUITERPENES;

CHEMISTRY;

ALKADIENE; AMINO ACID; AMORPHA 4,11 DIENE SYNTHASE; BETA FARNESENE; FARNESYL DIPHOSPHATE; METHYL GROUP; NEROLIDYL DIPHOSPHATE; PYROPHOSPHATE; SESQUITERPENE; SESQUITERPENE DERIVATIVE; SYNTHETASE; UNCLASSIFIED DRUG; AMORPHA-4,11-DIENE; AMORPHA-4,11-DIENE SYNTHASE; ISOPRENOID PHOSPHATE; PLANT PROTEIN; PYROPHOSPHORIC ACID DERIVATIVE; TRANSFERASE;

ALLYLIC REARRANGEMENT; ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; CYCLIZATION; IN VITRO STUDY; ISOMERIZATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; ARTEMISIA ANNUA; BIOCATALYSIS; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; MASS FRAGMENTOGRAPHY; METABOLISM; MISSENSE MUTATION; STEREOISOMERISM;

ALKYL AND ARYL TRANSFERASES; ARTEMISIA ANNUA; BIOCATALYSIS; CYCLIZATION; DIPHOSPHATES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; KINETICS; MODELS, CHEMICAL; MOLECULAR STRUCTURE; MUTATION, MISSENSE; PLANT PROTEINS; POLYISOPRENYL PHOSPHATES; SESQUITERPENES; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 85002972343     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.6b01004     Document Type: Article

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