Comparative analysis and validation of the malachite green assay for the high throughput biochemical characterization of terpene synthases

MethodsX

Volumn 1, Issue , 2014, Pages e187-e196

  Vardakou, Maria ^a   Salmon, Melissa ^a   Faraldos, Juan A ^c   O'Maille, Paul E ^a,b  

^a JOHN INNES CENTRE   (United Kingdom)

^b INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^c CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Catalytic efficiency; Colorimetric assay; High throughput; Malachite green; Screening; Steady state kinetics; Terpene synthases; Turnover number

Indexed keywords

FARNESYL DIPHOSPHATE; HYDROCARBON; MALACHITE GREEN; PRENYLAMINE; PYROPHOSPHATE; PYROPHOSPHORIC ACID DERIVATIVE; SYNTHETASE; TERPENE; TERPENE SYNTHASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BIOCHEMISTRY; CATALYSIS; CHEMICAL ANALYSIS; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; GENE LIBRARY; HIGH THROUGHPUT SCREENING; MALACHITE GREEN ASSAY; MASS FRAGMENTOGRAPHY; RADIOACTIVITY; STEADY STATE; VALIDATION PROCESS;

EID: 84907551132     PISSN: None     EISSN: 22150161     Source Type: Journal    
DOI: 10.1016/j.mex.2014.08.007     Document Type: Article

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