GC-MS-based 13C metabolic flux analysis

Methods in Molecular Biology

Volumn 1191, Issue , 2014, Pages 165-174

  Becker, Judith ^a   Wittmann, Christoph ^a  

^a NONE

Author keywords

13C; Central metabolism; GC MS; In vivo pathway analysis; Metabolic flux; Metabolic network; Metabolite balancing; Modeling; OpenFlux; Tracer

Indexed keywords

CARBON 13; MINERAL; SODIUM CHLORIDE; CARBON;

ARTICLE; COMPUTER PROGRAM; GAS CHROMATOGRAPHY; HYDROLYSIS; MASS SPECTROMETRY; METABOLIC FLUX ANALYSIS; METABOLITE; NONHUMAN; PRIORITY JOURNAL; STEADY STATE; BIOLOGICAL MODEL; DEVICES; MASS FRAGMENTOGRAPHY; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; PROCEDURES;

CARBON ISOTOPES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; INDUSTRIAL MICROBIOLOGY; METABOLIC FLUX ANALYSIS; METABOLIC NETWORKS AND PATHWAYS; MODELS, BIOLOGICAL;

EID: 84921833977     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1170-7_10     Document Type: Article

References (13)

1. Becker J, Wittmann C (2012) Pathways at work—metabolic fl ux analysis of the industrial cell factory Corynebacterium glutamicum. In: Yukawa H, Masayuki I (eds) Corynebacterium glutamicum, 1st edn. Springer, Berlin
2. Wiechert W (2001) 13 C metabolic fl ux analysis. Metab Eng 3(3):195–206. doi: 10.1006/ mben.2001.0187
3. Kelleher JK (2001) Flux estimations using isotopic tracers: common ground for metabolic physiology and metabolic engineering. Metab Eng 3:100–110. doi: 10.1006/mben.2001.0185
4. Wittmann C (2007) Fluxome analysis using GC-MS. Microb Cell Fact 6:6. doi: 10.1186/ 1475-2859-6-6
5. Kohlstedt M, Becker J, Wittmann C (2010) Metabolic fl uxes and beyond-systems biology understanding and engineering of microbial metabolism. Appl Microbiol Biotechnol 88(5): 1065–1075. doi: 10.1007/s00253-010-2854-2
6. Christensen B, Nielsen J (1999) Isotopomer analysis using GC-MS. Metab Eng 1(4):282–290. doi: 10.1006/mben.1999.0117
7. Wittmann C (2002) Metabolic fl ux analysis using mass spectrometry. Adv Biochem Eng Biotechnol 74:39–64
8. Quek L-E, Wittmann C, Nielsen LK et al (2009) OpenFLUX: effi cient modelling software for 13 C-based metabolic fl ux analysis. Microb Cell Fact 8:25. doi: 10.1186/1475-2859-8-25
9. Wittmann C, Hans M, Heinzle E (2002) In vivo analysis of intracellular amino acid labelings by GC/MS. Anal Biochem 307(2): 379–382
10. Becker J, Klopprogge C, Wittmann C (2008) Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum. Microb Cell Fact 7:8. doi: 10.1186/ 1475-2859-7-8
11. Becker J, Klopprogge C, Schröder H et al (2009) Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum. Appl Environ Microbiol 75(24):7866–7869. doi: 10.1128/AEM.01942-09
12. Kind S, Becker J, Wittmann C (2013) Increased lysine production by fl ux coupling of tricarboxylic acid cycle and biosynthetic pathway— metabolic engineering of succinyl-CoA availability in Corynebacterium glutamicum. Metab Eng 15:184–195
13. van Winden WA, Wittmann C, Heinzle E et al (2002) Correcting mass isotopomer distributions for naturally occurring isotopes. Biotechnol Bioeng 80(4):477–479. doi: 10.1002/ bit.10393