High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize

BMC Biotechnology

Volumn 16, Issue 1, 2016, Pages

  Qi, Weiwei ^a,c   Zhu, Tong ^a   Tian, Zhongrui ^a   Li, Chaobin ^a   Zhang, Wei ^a   Song, Rentao ^a,b,c  

^a SHANGHAI UNIVERSITY   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

^c Coordinated Crop Biology Research Center ^*  (China)

Author keywords

CRISPR Cas9; Maize; Multiplex gene editing; TRNA processing

Indexed keywords

AMINO ACIDS; EFFICIENCY;

CRISPR/CAS9; EXPRESSION CASSETTE; GENETIC RESEARCH; HIGH-EFFICIENCY; MAIZE; NON-CODING RNAS; PROCESSING SYSTEMS; SYNTHETIC GENES;

GENES;

CRISPR ASSOCIATED PROTEIN; LONG UNTRANSLATED RNA; TRANSFER RNA; GLYCINE; PLANT PROTEIN;

ACCURACY; ARTICLE; CAS9 GENE; CHROMOSOME DELETION; CONTROLLED STUDY; CRISPR GENE; GENE; GENE CASSETTE; GENE FUNCTION; GENETICS; MAIZE; MULTIPLEX GENE EDITING; NONHUMAN; PLANT BREEDING; PROCESS OPTIMIZATION; RNA PROCESSING; RNA SYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE EDITING; PLANT GENE; PROCEDURES; TRANSGENIC PLANT;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; GENE EDITING; GENES, PLANT; GLYCINE; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RNA, TRANSFER; ZEA MAYS;

EID: 84991826622     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-016-0289-2     Document Type: Article

References (33)

1. Wiedenheft B, Sternberg SH, Doudna JA. RNA guided genetic silencing systems in bacteria and archaea. Nature. 2012;7385:331-8.
2. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;6096:816-21.
3. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. RNA-guided human genome engineering via Cas9. Science. 2013;6121:823-6.
4. DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res. 2013;7:4336-43.
5. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013;3:227-9.
6. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM. Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013;194:1029-35.
7. Cho SW, Kim S, Kim JM, Kim JS. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013;3:230-2.
8. Jiang W, Zhou H, Bi H, Fromm M, Yang B, Weeks DP. Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Res. 2013;41:e188.
9. Kumar V, Jain M. The CRISPR-Cas system for plant genome editing: advances and opportunities. J Exp Bot. 2015;66:47-57.
10. Liang Z, Zhang K, Chen K, Gao C. Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. J Genet Genomics. 2014;41:63-8.
11. Svitashev S, Young JK, Schwartz C, Gao H, Falco SC. Cigan AMTargeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA. Plant Physiol. 2015;169:931-45.
12. Zhu J, Song N, Sun S, Yang W, Zhao H, Song W, Lai J. Efficiency and Inheritance of targeted mutagenesis in maize using CRISPR-Cas9. J Genet Genomics. 2016;43:25-36.
13. Feng C, Yuan J, Wang R, Liu Y, Birchler JA, Han F. Efficient targeted genome modification in maize using CRISPR/Cas9 System. J Genet Genomics. 2016; 43:37-43.
14. Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA. Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnol. 2015;15:16.
15. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;6121:819-23.
16. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 2013;4:910-8.
17. Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J. Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013;31(8):688-91.
18. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013;31(8):686-8.
19. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014;14:327.
20. Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu YG. A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plant. Mol Plant. 2015;8:1274.
21. Xie K, Minkenberg B, Yang Y. Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015;112:3570-5.
22. Frame BR, Shou H, Chikwamba RK, Zhang Z, Xiang C, Fonger TM, Pegg SEK, Li B, Nettleton DS, Pei D. Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system. Plant Physiol. 2002;129:13-22.
23. Gao H, Smith J, Yang M, Jones S, Djukanovic V, Nicholson MG, West A, Bidney D, Falco SC, Jantz D, et al. Heritable targeted mutagenesis in maize using a designed endonuclease. Plant J. 2010;61:176-87.
24. Wallace JC, Lopes MA, Paiva E, Larkins BA. New methods for extraction and quantitation of zeins reveal a high content of γ-zein in modified opaque-2 maize. Plant Physiol. 1990;92:191-6.
25. Xie K, Yang Y. RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant. 2013;6(6):1975-83.
26. Li C, Qiao Z, Qi W, Wang Q, Yuan Y, et al. Genome-Wide Characterization of cis-Acting DNA Targets Reveals the Transcriptional Regulatory Framework of Opaque2 in Maize. Plant Cell. 2015;27:532-45.
27. Qiao Z, Qi W, Wang Q, Feng Y, Yang Q, Zhang N, Wang S, Tang Y, Song R. ZmMADS47 regulates zein gene transcription through interaction with Opaque2. Plos Genet. 2016. doi:10.1371/journal.pgen.1005991.
28. Li XJ, Zhang YF, Mm H, Sun F, Shen Y, Xiu ZH, Wang XM, Chen ZL, Sun S, Small I, Tan BC. Small kernel 1 encodes a pentatricopeptide repeat protein required for mitochondrial nad7 transcript editing and seed development in maize (Zea mays) and rice (Oryza sativa). Plant J. 2014;79:797-809.
29. Sun F, Wang XM, Bonnard G, Shen Y, Xiu Z, Li X, Gao D, Zhang Z, Tan BC. Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmFN editing, mitochondrial function and seed development in maize. Plant J. 2015;84:283-95.
30. Forster AC, Altman S. External guide sequences for an RNA enzyme. Science. 1990;249(4970):783-6.
31. Johnson RA, Gurevich V, Filler S, Samach A, Levy AA. Comparative assessments of CRISPR-Cas nucleases' cleavage efficiency in planta. Plant Mol Biol. 2015;87:143e156.
32. Fauser F, Schiml S, Puchta H. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014;79:348e359.
33. Zhang H, Zhang J, Wei P, Zhang B, Gou F, Feng Z, Mao Y, Yang L, Zhang H, Xu N, Zhu J. The CRISPR/Cas9 system produces specific and homozygous targete gene editing in rice in one gerneration. Plant Biotech J. 2014;12: 797e807.