Is low non-Lethal concentration of methylmercury really safe? A report on genotoxicity with delayed cell proliferation

PLoS ONE

Volumn 11, Issue 9, 2016, Pages

  Crespo Lopez, María Elena ^a   Costa Malaquias, Allan ^a   Oliveira, Edivaldo H C ^b   Miranda, Moysés S ^a   Arrifano, Gabriela P F ^a   Souza Monteiro, José R ^a   Sagica, Fernanda Espirito Santo ^b   Fontes Junior, Enéas A ^a   Maia, Cristiane S F ^a   Macchi, Barbarella M ^a   Do Nascimento, José Luiz M ^a  

^a FEDERAL UNIVERSITY OF PARÁ   (Brazil)

^b INSTITUTO EVANDRO CHAGAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

METHYLMERCURY; METHYLMERCURY DERIVATIVE; MUTAGENIC AGENT;

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOACCUMULATION; CELL POPULATION; CELL PROLIFERATION; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA FRAGMENTATION; G2 PHASE CELL CYCLE CHECKPOINT; GENOTOXICITY; MICRONUCLEUS; MITOCHONDRION; NONHUMAN; NUCLEAR BUD; NUCLEOPLASMIC BRIDGE; RAT; S PHASE CELL CYCLE CHECKPOINT; ANIMAL; CELL CYCLE; CELL LINE; CELL SURVIVAL; DNA DAMAGE; DRUG EFFECTS; GLIA; HUMAN; METABOLISM; MUTAGEN TESTING; PATHOLOGY;

ANIMALS; APOPTOSIS; CELL CYCLE; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; DNA DAMAGE; HUMANS; METHYLMERCURY COMPOUNDS; MUTAGENICITY TESTS; MUTAGENS; NEUROGLIA; RATS;

EID: 84990901996     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0162822     Document Type: Article

References (46)

1. WHO-World Health Organization. Resolution of January, 23. 2014. Available from: http://apps.who. int/gb/ebwha/pdf-files/EB134/B134-R5-en.pdf
2. WHO-World Health Organization. Environmental health criteria: methylmercury. InternationalProgram on Chemical Safety, Geneva1990. p. 101-40.
3. USEPA-United States Environmental Protection Agency. Mercury study report to congress. Health effects of mercury and mercury compounds. Washington1997.
4. Crespo-Lopez ME, Herculano AM, Corvelo TC, Do Nascimento JL. [Mercury and neurotoxicity]. Revista de neurologia. 2005; 40(7):441-7. Epub 2005/04/26. PMID: 15849680.
5. Berzas Nevado JJ, Rodriguez Martin-Doimeadios RC, Guzman Bernardo FJ, Jimenez Moreno M, Herculano AM, do Nascimento JL, et al. Mercury in the Tapajos River basin, Brazilian Amazon: a review. Environment international. 2010; 36(6):593-608. Epub 2010/05/21. doi: 10.1016/j.envint.2010.03.011 PMID: 20483161.
6. Karagas MR, Choi AL, Oken E, Horvat M, Schoeny R, Kamai E, et al. Evidence on the human health effects of low-level methylmercury exposure. Environmental health perspectives. 2012; 120(6):799-806. Epub 2012/01/26. doi: 10.1289/ehp.1104494 PMID: 22275730; PubMed Central PMCID: PMC3385440.
7. Rodriguez Martin-Doimeadios RC, Berzas Nevado JJ, Guzman Bernardo FJ, Jimenez Moreno M, Arrifano GP, Herculano AM, et al. Comparative study of mercury speciation in commercial fishes of the Brazilian Amazon. Environmental science and pollution research international. 2014; 21(12):7466-79. Epub 2014/03/05. doi: 10.1007/s11356-014-2680-7 PMID: 24590602.
8. Crespo-Lopez ME, Macedo GL, Pereira SI, Arrifano GP, Picanco-Diniz DL, do Nascimento JL, et al. Mercury and human genotoxicity: critical considerations and possible molecular mechanisms. Pharmacological research. 2009; 60(4):212-20. Epub 2009/05/19. doi: 10.1016/j.phrs.2009.02.011 PMID: 19446469.
9. Crespo-Lopez ME, Macedo GL, Arrifano GP, Pinheiro Mda C, do Nascimento JL, Herculano AM. Genotoxicity of mercury: contributing for the analysis of Amazonian populations. Environment international. 2011; 37(1):136-41. Epub 2010/09/10. doi: 10.1016/j.envint.2010.08.009 PMID: 20825993.
10. Berzas Nevado JJ RM-D R, Jiménez Moreno M, Martins do Nascimento JL, Herculano AM, Crespo-López ME Mercury speciation analysis on cell lines of the human central nervous system to explain genotoxic effects. Microchemical Journal. 2009; 93:12-9. doi: 10.1016/j.microc.2009.03.008
11. Crespo-Lopez ME, Lima de Sa A, Herculano AM, Rodriguez Burbano R, Martinsdo Nascimento JL. Methylmercury genotoxicity: a novel effect in human cell lines of the central nervous system. Environment international. 2007; 33(2):141-6. Epub 2006/09/30. doi: 10.1016/j.envint.2006.08.005 PMID: 17007929.
12. Miura K, Koide N, Himeno S, Nakagawa I, Imura N. The involvement of microtubular disruption in methylmercury-induced apoptosis in neuronal and nonneuronal cell lines. Toxicology and applied pharmacology. 1999; 160(3):279-88. Epub 1999/11/02. doi: 10.1006/taap.1999.8781 PMID: 10544062.
13. Burke K, Cheng Y, Li B, Petrov A, Joshi P, Berman RF, et al. Methylmercury elicits rapid inhibition of cell proliferation in the developing brain and decreases cell cycle regulator, cyclin E. Neurotoxicology. 2006; 27(6):970-81. Epub 2006/10/24. doi: 10.1016/j.neuro.2006.09.001 PMID: 17056119; PubMed Central PMCID: PMC2013736.
14. Mundy WR, Radio NM, Freudenrich TM. Neuronal models for evaluation of proliferation in vitro using high content screening. Toxicology. 2010; 270(2-3):121-30. Epub 2010/02/13. doi: 10.1016/j.tox. 2010.02.004 PMID: 20149836.
15. Xu M, Yan C, Tian Y, Yuan X, Shen X. Effects of low level of methylmercury on proliferation of cortical progenitor cells. Brain research. 2010; 1359:272-80. Epub 2010/09/04. doi: 10.1016/j.brainres.2010. 08.069 PMID: 20813099.
16. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods. 1983; 65(1-2):55-63. Epub 1983/12/16. PMID: 6606682.
17. Takahashi A, Matsumoto H, Yuki K, Yasumoto J, Kajiwara A, Aoki M, et al. High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status. International journal of radiation oncology, biology, physics. 2004; 60(2):591-7. Epub 2004/09/24. doi: 10.1016/j.ijrobp.2004.05.062 PMID: 15380596.
18. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental cell research. 1988; 175(1):184-91. Epub 1988/03/01. PMID: 3345800.
19. Strober W. Trypan blue exclusion test of cell viability. Current protocols in immunology / edited by John E Coligan [et al]. 2001; Appendix 3:Appendix 3B. Epub 2008/04/25. doi: 10.1002/0471142735. ima03bs21 PMID: 18432654.
20. Roth V. 2006. Available from: http://www.doubling-time.com/compute.php.
21. Sanfeliu C, Sebastia J, Ki SU. Methylmercury neurotoxicity in cultures of human neurons, astrocytes, neuroblastoma cells. Neurotoxicology. 2001; 22(3):317-27. Epub 2001/07/18. PMID: 11456333.
22. Costa-Malaquias A, Almeida MB, Souza Monteiro JR, Macchi Bde M, do Nascimento JL, Crespo-Lopez ME. Morphine protects against methylmercury intoxication: a role for opioid receptors in oxidative stress? PloS one. 2014; 9(10):e110815. Epub 2014/10/21. doi: 10.1371/journal.pone.0110815 PMID: 25330195; PubMed Central PMCID: PMC4201572.
23. Pieper I, Wehe CA, Bornhorst J, Ebert F, Leffers L, Holtkamp M, et al. Mechanisms of Hg species induced toxicity in cultured human astrocytes: genotoxicity and DNA-damage response. Metallomics: integrated biometal science. 2014; 6(3):662-71. Epub 2014/02/20. doi: 10.1039/c3mt00337j PMID: 24549367.
24. Sumantran VN. Cellular chemosensitivity assays: an overview. Methods Mol Biol. 2011; 731:219-36. Epub 2011/04/26. doi: 10.1007/978-1-61779-080-5-19 PMID: 21516411.
25. Dare E, Li W, Zhivotovsky B, Yuan X, Ceccatelli S. Methylmercury and H(2)O(2) provoke lysosomal damage in human astrocytoma D384 cells followed by apoptosis. Free radical biology & medicine. 2001; 30(12):1347-56. Epub 2001/06/08. PMID: 11390179.
26. Das S, Nageshwar Rao B, Satish Rao BS. Mangiferin attenuates methylmercury induced cytotoxicity against IMR-32, human neuroblastoma cells by the inhibition of oxidative stress and free radical scavenging potential. Chemico-biological interactions. 2011; 193(2):129-40. Epub 2011/06/28. doi: 10. 1016/j.cbi.2011.06.002 PMID: 21703249.
27. Fenech M, Kirsch-Volders M, Natarajan AT, Surralles J, Crott JW, Parry J, et al. Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis. 2011; 26(1):125-32. Epub 2010/12/18. doi: 10.1093/mutage/geq052 PMID: 21164193.
28. Thomas P, Umegaki K, Fenech M. Nucleoplasmic bridges are a sensitive measure of chromosome rearrangement in the cytokinesis-block micronucleus assay. Mutagenesis. 2003; 18(2):187-94. Epub 2003/03/07. PMID: 12621075.
29. Huyck RW, Nagarkar M, Olsen N, Clamons SE, Saha MS. Methylmercury exposure during early Xenopus laevis development affects cell proliferation and death but not neural progenitor specification. Neurotoxicology and teratology. 2015; 47:102-13. Epub 2014/12/17. doi: 10.1016/j.ntt.2014.11.010 PMID: 25496965; PubMed Central PMCID: PMC4302163.
30. Hong JY, Chung HJ, Bae SY, Trung TN, Bae K, Lee SK. Induction of Cell Cycle Arrest and Apoptosis by Physcion, an Anthraquinone Isolated From Rhubarb (Rhizomes of Rheum tanguticum), in MDA-MB-231 Human Breast Cancer Cells. Journal of cancer prevention. 2014; 19(4):273-8. Epub 2015/01/13. doi: 10.15430/JCP.2014.19.4.273 PMID: 25574462; PubMed Central PMCID: PMC4285958.
31. Zhang P, Xu Y, Sun J, Li X, Wang L, Jin L. Protection of pyrroloquinoline quinone against methylmercury-induced neurotoxicity via reducing oxidative stress. Free radical research. 2009; 43(3):224-33. Epub 2009/02/05. doi: 10.1080/10715760802677348 PMID: 19191107.
32. Mendoza MA, Ponce RA, Ou YC, Faustman EM. p21(WAF1/CIP1) inhibits cell cycle progression but not G2/M-phase transition following methylmercury exposure. Toxicology and applied pharmacology. 2002; 178(2):117-25. Epub 2002/01/30. doi: 10.1006/taap.2001.9267 PMID: 11814332.
33. Ponce RA, Kavanagh TJ, Mottet NK, Whittaker SG, Faustman EM. Effects of methyl mercury on the cell cycle of primaryrat CNS cells in vitro. Toxicology and applied pharmacology. 1994; 127(1):83-90. Epub 1994/07/01. doi: 10.1006/taap.1994.1142 PMID: 8048057.
34. Bose R, Onishchenko N, Edoff K, Janson Lang AM, Ceccatelli S. Inherited effects of low-dose exposure to methylmercury in neural stem cells. Toxicological sciences: an official journal of the Society of Toxicology. 2012; 130(2):383-90. Epub 2012/08/25. doi: 10.1093/toxsci/kfs257 PMID: 22918959.
35. Ceccatelli S, Bose R, Edoff K, Onishchenko N, Spulber S. Long-lasting neurotoxic effects of exposure to methylmercury during development. Journal of internal medicine. 2013; 273(5):490-7. Epub 2013/ 04/23. doi: 10.1111/joim.12045 PMID: 23600401.
36. Sokolowski K, Obiorah M, Robinson K, McCandlish E, Buckley B, DiCicco-Bloom E. Neural stem cell apoptosis after low-methylmercury exposures in postnatal hippocampus produce persistent cell loss and adolescent memory deficits. Developmental neurobiology. 2013; 73(12):936-49. Epub 2013/08/ 21. doi: 10.1002/dneu.22119 PMID: 23959606; PubMed Central PMCID: PMC3874131.
37. Ceccatelli S, Dare E, Moors M. Methylmercury-induced neurotoxicity and apoptosis. Chemico-biological interactions. 2010; 188(2):301-8. Epub 2010/04/20. doi: 10.1016/j.cbi.2010.04.007 PMID: 20399200.
38. Belletti S, Orlandini G, Vettori MV, Mutti A, Uggeri J, Scandroglio R, et al. Time course assessment of methylmercury effects on C6 glioma cells: submicromolar concentrations induce oxidative DNA damage and apoptosis. Journal of neuroscience research. 2002; 70(5):703-11. Epub 2002/11/09. doi: 10. 1002/jnr.10419 PMID: 12424738.
39. Morris MC, Brockman J, Schneider JA, Wang Y, Bennett DA, Tangney CC, et al. Association of Seafood Consumption, Brain Mercury Level, and APOE epsilon4 Status With Brain Neuropathology in Older Adults. Jama. 2016; 315(5):489-97. Epub 2016/02/03. doi: 10.1001/jama.2015.19451 PMID: 26836731.
40. Falnoga I, Tusek-Znidaric M, Horvat M, Stegnar P. Mercury, selenium, and cadmium in human autopsy samples from Idrija residents and mercury mine workers. Environmental research. 2000; 84(3):211-8. Epub 2000/12/01. doi: 10.1006/enrs.2000.4116 PMID: 11097794.
41. Magos L. The absorption, distribution and excretion of methylmercury. In: Eccles CU A Z, editor. The toxicity of methylmercury. Baltimore: Johns Hopkins; 1987. p. 24-44.
42. Burbacher TM, Rodier PM, Weiss B. Methylmercury developmental neurotoxicity: a comparison of effects in humans and animals. Neurotoxicology and teratology. 1990; 12(3):191-202. Epub 1990/05/ 01. PMID: 2196419.
43. Harada M, Nakanishi J, Yasoda E, Pinheiro MC, Oikawa T, de Assis Guimaraes G, et al. Mercury pollution in the Tapajos River basin, Amazon: mercury level of head hair and health effects. Environment international. 2001; 27(4):285-90. Epub 2001/11/01. PMID: 11686639.
44. Carta P, Flore C, Alinovi R, Ibba A, Tocco MG, Aru G, et al. Sub-clinical neurobehavioral abnormalities associated with low level of mercury exposure through fish consumption. Neurotoxicology. 2003; 24(4-5):617-23. Epub 2003/08/06. doi: 10.1016/S0161-813X(03)00080-9 PMID: 12900074.
45. Auger N, Kofman O, Kosatsky T, Armstrong B. Low-level methylmercury exposure as a risk factor for neurologic abnormalities in adults. Neurotoxicology. 2005; 26(2):149-57. Epub 2005/02/17. doi: 10. 1016/j.neuro.2004.12.005 PMID: 15713336.
46. Pinheiro MC, Crespo-Lopez ME, Vieira JL, Oikawa T, Guimaraes GA, Araujo CC, et al. Mercury pollution and childhood in Amazon riverside villages. Environment international. 2007; 33(1):56-61. Epub 2006/08/26. doi: 10.1016/j.envint.2006.06.024 PMID: 16930706.