CRISPR/Cas9-Mediated genome editing of mouse small intestinal organoids

Methods in Molecular Biology

Volumn 1422, Issue , 2016, Pages 3-11

  Schwank, Gerald ^a   Clevers, Hans ^a  

^a NONE

Author keywords

CRISPR Cas9; Genome editing; Intestinal stem cells; Small intestinal organoids

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOPLASM; GENE FUNCTION; GENOME; HOMEOSTASIS; INTESTINAL STEM CELL; INTESTINE EPITHELIUM; NONHUMAN; PATHOPHYSIOLOGY; PRIORITY JOURNAL; SMALL INTESTINE; SOMATIC CELL; STEM CELL SELF-RENEWAL; ANIMAL; CRISPR CAS SYSTEM; CYTOLOGY; GENE EDITING; MOUSE; ORGAN CULTURE TECHNIQUE;

ANIMALS; CRISPR-CAS SYSTEMS; GENE EDITING; INTESTINE, SMALL; MICE; ORGAN CULTURE TECHNIQUES;

EID: 84990040785     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3603-8_1     Document Type: Article

References (20)

1. Bhaya D, Davison M, Barrangou R (2011) CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. Annu Rev Genet 45:273-297
2. Sorek R, Lawrence CM, Wiedenheft B (2013) CRISPR-mediated adaptive immune systems in bacteria and archaea. Annu Rev Biochem 82:237-266
3. Sander JD, Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Nat Biotechnol 32:347-355
4. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816-821
5. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8:2281-2308
6. Cong L, Ran FA, Cox D, Lin S et al (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819-823
7. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM (2013) RNA-guided human genome engineering via Cas9. Science 339:823-826
8. Sato T, Vries RG, Snippert HJ, van de Wetering M et al (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262-265
9. Boj SF, Hwang CI, Baker LA, Chio II et al (2015) Organoid models of human and mouse ductal pancreatic cancer. Cell 160:324-338
10. Huch M, Bonfanti P, Boj SF, Sato T et al (2013) Unlimited in vitro expansion of adult bipotent pancreas progenitors through the Lgr5/ R-spondin axis. EMBO J 32:2708-2721
11. Jung P, Sato T, Merlos-Suarez A, Barriga FM et al (2011) Isolation and in vitro expansion of human colonic stem cells. Nat Med 17:1225-1227
12. Sato T, Stange DE, Ferrante M, Vries RG et al (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology 141:1762-1772
13. Huch M, Gehart H, van Boxtel R, Hamer K et al (2015) Long-term culture of genomestable bipotent stem cells from adult human liver. Cell 160:299-312
14. Drost J, van Jaarsveld RH, Ponsioen B, Zimberlin C et al (2015) Sequential cancer mutations in cultured human intestinal stem cells. Nature 521:43-47
15. Matano M, Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T (2015) Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Nat Med 21:256-262
16. Schwank G, Koo BK, Sasselli V, Dekkers JF et al (2013) Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cell Stem Cell 13:653-658
17. Sato T, Clevers H (2013) Primary mouse small intestinal epithelial cell cultures. Methods Mol Biol 945:319-328
18. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR et al (2015) Efficient intracellular delivery of native proteins. Cell 161:674-690
19. Yusa K, Rashid ST, Strick-Marchand H, Varela I et al (2011) Targeted gene correction of alpha1-antitrypsin deficiency in induced pluripotent stem cells. Nature 478:391-394
20. Willert K, Brown JD, Danenberg E, Duncan AW, Weissman IL, Reya T, Yates JR III, Nusse R (2003) Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423:448-452