Leapfrogging: Primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes

Development (Cambridge)

Volumn 143, Issue 15, 2016, Pages 2868-2875

  Blitz, Ira L ^a   Fish, Margaret B ^a   Cho, Ken W Y ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

CRISPR Cas9; Genome editing; Knockouts; Primordial germ cells; TALENs; Xenopus

Indexed keywords

ALLELE; AMPLICON; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLASTULA; CELL TRANSPLANTATION; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; EMBRYO; ENGRAFTMENT; ESSENTIAL GENE; EXON; FEMALE; GAMETE; GASTRULATION; GENE MUTATION; HETEROZYGOTE; IN SITU HYBRIDIZATION; INDEL MUTATION; LEAPFROGGING; MALE; MUTAGENESIS; NONHUMAN; NULL ALLELE; PHENOTYPE; PRIMORDIAL GERM CELL; PRIORITY JOURNAL; PROGENY; SEXUAL MATURITY; XENOPUS TROPICALIS; ANIMAL; ANURA; CYTOLOGY; GENETICS; GERM CELL; METABOLISM; MUTATION; NONMAMMALIAN EMBRYO; PHYSIOLOGY; XENOPUS;

TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE;

ANIMALS; ANURA; BLASTULA; CRISPR-CAS SYSTEMS; EMBRYO, NONMAMMALIAN; FEMALE; GERM CELLS; MALE; MUTAGENESIS; MUTATION; TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES; XENOPUS;

EID: 84982975971     PISSN: 09501991     EISSN: 14779129     Source Type: Journal    
DOI: 10.1242/dev.138057     Document Type: Article

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