Cas9-based genome editing in xenopus tropicalis

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 355-375

  Nakayama, Takuya ^a   Blitz, Ira L ^b   Fish, Margaret B ^b   Odeleye, Akinleye O ^a   Manohar, Sumanth ^a   Cho, Ken W Y ^b   Grainger, Robert M ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Amphibian mutagenesis; CRISPR; Disease model sgRNA design; Genome engineering; Loss of function; Off target effects; Targeted mutagenesis

Indexed keywords

CRISPR ASSOCIATED PROTEIN; MESSENGER RNA; GUIDE RNA;

ARTICLE; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; DNA MODIFICATION; DOUBLE STRANDED DNA BREAK; EMBRYO; GENE TARGETING; GENOME; GENOTYPE; HETEROZYGOTE; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; INDEL MUTATION; LOSS OF FUNCTION MUTATION; MICROINJECTION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PHENOTYPE; PRIORITY JOURNAL; PROTEIN CLEAVAGE; RNA TRANSCRIPTION; XENOPUS TROPICALIS; ANIMAL; ANIMAL EMBRYO; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; EMBRYOLOGY; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PROCEDURES; XENOPUS;

AMPHIBIA; XENOPUS TROPICALIS;

ANIMALS; BASE SEQUENCE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; EMBRYO, NONMAMMALIAN; GENE TARGETING; GENETIC ENGINEERING; GENOME; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RNA, GUIDE; XENOPUS;

EID: 84925457676     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00017-9     Document Type: Chapter

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