High-level intra- and extra-cellular production of d-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis

Journal of Industrial Microbiology and Biotechnology

Volumn 43, Issue 11, 2016, Pages 1577-1591

  Chen, Jingqi ^a,b   Zhu, Yueming ^a,c   Fu, Gang ^a,c   Song, Yafeng ^a,b   Jin, Zhaoxia ^d   Sun, Yuanxia ^a,c   Zhang, Dawei ^a,b,c  

^a Chinese Academy of Sciences   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c National Engineering Laboratory for Industrial Enzymes   (China)

^d DALIAN POLYTECHNIC UNIVERSITY   (China)

Author keywords

Bacillus subtilis; d Psicose 3 epimerase; Protein secretion; xylAB; Xylose inducible expression system

Indexed keywords

EPIMERASE; PSICOSE; SIGNAL PEPTIDE; XYLOSE; FRUCTOSE;

ARTICLE; BACILLUS SUBTILIS; BIOACCUMULATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; FED BATCH FERMENTATION; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; METABOLISM; NONHUMAN; PROMOTER REGION; REDUCTION; RUMINOCOCCUS; ENZYMOLOGY; GENETICS;

BACILLUS SUBTILIS; CARBOHYDRATE EPIMERASES; FRUCTOSE; PROMOTER REGIONS, GENETIC; RUMINOCOCCUS; XYLOSE;

EID: 84982300249     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-016-1819-6     Document Type: Article

References (43)

1. Mu W, Zhang W, Feng Y, Jiang B, Zhou L (2012) Recent advances on applications and biotechnological production of d-psicose. Appl Microbiol Biotechnol 94:1461–1467
2. Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B (2011) Cloning, expression, and characterization of a d-psicose 3-epimerase from Clostridium cellulolyticum H10. J Agric Food Chem 59:7785–7792
3. Oshima H, Kimura I, Izumori K (2006) Psicose contents in various food products and its origin. Food Sci Technol Res 12:137–143
4. Matsuo T, Suzuki H, Hashiguchi M, Izumori K (2002) d-Psicose is a rare sugar that provides no energy to growing rats. J Nutr Sci Vitaminol 48:77–80
5. Iida T, Kishimoto Y, Yoshikawa Y, Hayashi N, Okuma K, Tohi M, Yagi K, Matsuo T, Izumori K (2008) Acute d-psicose administration decreases the glycemic responses to an oral maltodextrin tolerance test in normal adults. J Nutr Sci Vitaminol 54:511–514
6. Hayashi N, Iida T, Yamada T, Okuma K, Takehara I, Yamamoto T, Yamada K, Tokuda M (2010) Study on the postprandial blood glucose suppression effect of d-psicose in borderline diabetes and the safety of long-term ingestion by normal human subjects. Biosci Biotechnol Biochem 74:510–519
7. Matsuo T, Baba Y, Hashiguchi M, Takeshita K, Izumori K, Suzuki H (2001) Less body fat accumulation with d-psicose diet versus d-fructose diet. J Clin Biochem Nutr 30:55–65
8. Sun Y, Hayakawa S, Izumori K (2004) Modification of ovalbumin with a rare ketohexose through the maillard reaction: effect on protein structure and gel properties. J Agric Food Chem 52:1293–1299
9. Izumori K, Khan AR, Okaya H, Tsumura T (1993) A new enzyme, d-ketohexose 3-epimerase, from Pseudomonas sp. ST-24. Biosci Biotechnol Biochem 57:1037–1039
10. Itoh H, Okaya H, Khan AR, Tajima S, Hayakawa S, Izumori K (1994) Purification and characterization of d-tagatose 3-epimerase from Pseudomonas sp. ST-24. Biosci Biotechnol Biochem 58:2168–2171
11. Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK (2006) Characterization of an Agrobacterium tumefaciens d-psicose 3-epimerase that converts d-fructose to d-psicose. Appl Environ Microbiol 72:981–985
12. Zhang L, Mu W, Jiang B, Zhang T (2009) Characterization of d-tagatose-3-epimerase from Rhodobacter sphaeroides that converts d-fructose into d-psicose. Biotechnol Lett 31:857–862
13. Zhu Y, Men Y, Bai W, Li X, Zhang L, Sun Y, Ma Y (2012) Overexpression of d-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in d-psicose production. Biotechnol Lett 34:1901–1906
14. Zhang WL, Fang D, Xing QC, Zhou L, Jiang B, Mu WM (2013) Characterization of a novel metal-dependent d-psicose 3-epimerase from Clostridium scindens 35704. PLoS One 8:e62987
15. Zhang W, Fang D, Zhang T, Zhou L, Jiang B, Mu W (2013) Characterization of a metal-dependent d-psicose 3-epimerase from a novel strain, Desmospora sp. 8437. J Agric Food Chem 61:11468–11476
16. Jia M, Mu W, Chu F, Zhang X, Jiang B, Zhou LL, Zhang T (2013) A d-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for d-psicose production: cloning, expression, purification, and characterization. Appl Microbiol Biotechnol 98:717–725
17. Westers L, Westers H, Quax WJ (2004) Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 1694:299–310
18. Blattner FR, Plunkett G, Bloch CA (1997) The complete genome sequence of Escherichia coli K-12. Science 277:1453–1462
19. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G (1997) The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390:249–256
20. Sorensen HP, Mortensen KK (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115:113–128
21. Nijland R, Kuipers OP (2008) Optimization of protein secretion by Bacillus subtilis. Recent Pat Biotechnol 2:79–87
22. Simonen M, Palva I (1993) Protein secretion in Bacillus species. Microbiol Rev 57:109–137
23. Hanvood CR (1992) Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotechnol 10:247–256
24. Chen J, Gai Y, Fu G, Zhou W, Zhang D, Wen J (2015) Enhanced extracellular production of alpha-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnol Lett 37:899–906
25. Chen J, Fu G, Gai Y, Zheng P, Zhang D, Wen J (2015) Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression. Microb Cell Fact 14:92
26. Green MR, Sambrook J (2012) Molecular cloning: a laboratory manual, 4th edn. Cold Spring Harbor Laboratory Press, New York
27. Cr H, Sm C (1990) Molecular biological methods for Bacillus. Wiley, Chichester
28. Spizizen J (1958) Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc Natl Acad Sci USA 44:1072–1078
29. You C, Zhang XZ, Zhang YH (2012) Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis. Appl Environ Microbiol 78:1593–1595
30. Liu S, Endo K, Ara K, Ozaki K, Ogasawara N (2008) Introduction of marker-free deletions in Bacillus subtilis using the AraR repressor and the ara promoter. Microbiology 154:2562–2570
31. Guerra LT, Xu Y, Bennette N, McNeely K, Bryant DA, Dismukes GC (2013) Natural osmolytes are much less effective substrates than glycogen for catabolic energy production in the marine cyanobacterium Synechococcus sp. strain PCC 7002. J Biotechnol 166:65–75
32. Radha S, Gunasekaran P (2008) Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941. Bioresour Technol 99:5528–5537
33. Ming YM, Wei ZW, Lin CY, Sheng GY (2010) Development of a Bacillus subtilis expression system using the improved Pglv promoter. Microb Cell Fact 9:55
34. Liu SL, Du K (2012) Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme. Protein Expr Purif 83:164–168
35. Chen T, Liu WX, Fu J, Zhang B, Tang YJ (2013) Engineering Bacillus subtilis for acetoin production from glucose and xylose mixtures. J Biotechnol 168:499–505
36. Zhang B, Li N, Wang Z, Tang YJ, Chen T, Zhao X (2015) Inverse metabolic engineering of Bacillus subtilis for xylose utilization based on adaptive evolution and whole-genome sequencing. Appl Microbiol Biotechnol 99:885–896
37. Park YC, Jun SY, Seo JH (2012) Construction and characterization of recombinant Bacillus subtilis JY123 able to transport xylose efficiently. J Biotechnol 161:402–406
38. Phan TT, Tran LT, Schumann W, Nguyen HD (2015) Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli. Microb Cell Fact 14:72
39. Bendtsen JD, Nielsen H, Widdick D, Palmer T, Brunak S (2005) Prediction of twin-arginine signal peptides. BMC Bioinf 6:167
40. Phan TT, Nguyen HD, Schumann W (2006) Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr Purif 46:189–195
41. Bhavsar AP, Zhao X, Brown ED (2001) Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant. Appl Environ Microbiol 67:403–410
42. Vavrova L, Muchova K, Barak I (2010) Comparison of different Bacillus subtilis expression systems. Res Microbiol 161:791–797
43. Biedendieck R, Bunk B, Furch T, Franco-Lara E, Jahn M, Jahn D (2010) Systems biology of recombinant protein production in Bacillus megaterium. Adv Biochem Eng Biotechnol 120:133–161