Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: Identification of bottlenecks by systematic gene overexpression

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Chen, Jingqi ^a,b,c   Fu, Gang ^b,d   Gai, Yuanming ^b,d   Zheng, Ping ^b,c   Zhang, Dawei ^b,c,d   Wen, Jianping ^a  

^a TIANJIN UNIVERSITY   (China)

^b Chinese Academy of Sciences   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^d National Engineering Laboratory for Industrial Enzymes   (China)

Author keywords

Bacillus subtilis; Bottleneck; Heterologous protein; Overexpression; Sec pathway

Indexed keywords

PROTEIN DNAK; RECOMBINANT ALPHA AMYLASE L; RECOMBINANT ALPHA AMYLASE S; RECOMBINANT ENZYME; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; XYLOSE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BOTTLENECK POPULATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNAK OPERON; ENZYME REGULATION; FED BATCH FERMENTATION; GENE DOSAGE; GENE INDUCTION; GENE OVEREXPRESSION; GENETIC RECOMBINATION; HETEROLOGOUS EXPRESSION; MOLECULAR GENETICS; NONHUMAN; OPERON; PRODUCTIVITY; PROMOTER REGION; PROTEIN DEFICIENCY; PROTEIN SECRETION; PRSA GENE; SECRETORY PATHWAY; GENE EXPRESSION; GENETICS; METABOLISM; PROTEIN TRANSPORT;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; GENE EXPRESSION; PROMOTER REGIONS, GENETIC; PROTEIN TRANSPORT;

EID: 84933510021     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0282-9     Document Type: Article

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