Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941

Bioresource Technology

Volumn 99, Issue 13, 2008, Pages 5528-5537

  Radha, S ^a   Gunasekaran, P ^a  

^a Center for Excellence in Genomic Sciences   (India)

Author keywords

Bacillius megaterium; Bacillus licheniformis; Keratinase; PamyL; PxylA

Indexed keywords

ENZYMES; GENE ENCODING; GENE EXPRESSION; XYLOSE;

BACILLIUS MEGATERIUM; BACILLUS LICHENIFORMIS; KERATINASE;

BACTERIA;

AMYLASE; KERATINASE; STARCH; XYLOSE;

BACTERIA; ENZYMES; GENE ENCODING; GENE EXPRESSION; XYLOSE;

BACTERIUM; COMPARATIVE STUDY; ENZYME ACTIVITY; GENE EXPRESSION; PLASMID; RECOMBINATION; STARCH; SUGAR;

ARTICLE; BACILLUS MEGATERIUM; BACTERIUM CULTURE; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

ALPHA-AMYLASE; BACILLUS; BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GRAM-NEGATIVE BACTERIA; PEPTIDE HYDROLASES; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS;

BACILLUS LICHENIFORMIS; BACILLUS MEGATERIUM; ESCHERICHIA COLI;

EID: 43049138084     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2007.10.052     Document Type: Article

References (48)

1. Anbu P., Lakshmipriya S.C.B.G., and Annadurai G. Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis. Bioresour. Technol. 98 (2007) 1298-1303
2. Balint B. Biohydrogen production from keratin-containing animal wastes. Acta Biologica Szegediensis 50 (2006) 137
3. Bertsch A., and Coello N. A biotechnological process for treatment and recycling poultry feathers as a feed ingredient. Bioresour. Technol. 96 (2005) 1703-1708
4. Bockle B., Galunsky B., and Muller R. Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl. Environ. Microbiol. 61 (1995) 3705-3710
5. Bressollier P., Letourneau F., Urdaci M., and Verneuil B. Purification and Characterization of a keratinolytic serine proteinase form Streptomyces albidoflavus. Appl. Environ. Microbiol. 65 (1999) 2570-2576
6. Brockmeier U., Wendorff M., and Eggert T. Versatile expression and secretion vectors for Bacillus subtilis. Curr. Microbiol. 52 (2006) 143-148
7. Chak K.F., Tseng M.Y., and Yamamoto T. Expression of the cystal protein gene under the control of the of α-amylase promoter in Bacillus thuringiensis strains. Appl. Environ. Microbiol. 60 (1994) 2304-2310
8. Chitte R.R., Nalawade V.K., and Dey S. Keratinolytic activity from the broth of a feather-degrading thermophilic Sreptomyces thermoviolaceus strain SD8. Letts. Appl. Microbiol. 28 (1999) 131-136
9. Devine K.M., Hogan S.T., Higgins D.G., and McConnell D.J. Replication and segregational stability of Bacillus plasmid pBAA1. J. Bacteriol. 171 (1989) 1166-1172
10. Fahnestock S.R., and Fisher K.E. Expression of the staphylococcal protein A Gene in Bacillus subtilis by gene fusions utilizing the promoter from a Bacillus amyloliquefaciens α-amylase Gene. J. Bacteriol. 165 (1986) 796-804
11. Gradisar H., Friedrich J., Krizaj I., and Jerala1 R. Similarities and specificities of fungal keratinolytic proteases: comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases. Appl. Environ. Microbiol. 71 (2005) 3420-3426
12. Gushterova A., Tonkova E.V., Dimova E., Nedkov P., and Haertle T. Keratinase production by newly isolated Antarctic actinomycete strains. World J. Mcrobiol. Microbiol. Biotechnol. 21 (2005) 831-834
13. Kaul S., and Sumbali G. Keratinolysis by poultry farm soil fungi. Mycopathologia 139 (1997) 137-140
14. Kluskens L.D., Voorhorst W.G.B., Siezen R.J., Schwerdtfeger M., Antranikian G., Oost J.V., and deVos W.M. Molecular characterization of fervidolysin, a subtilisin like serine protease from the thermophilic bacterium Fervidobacterium pennivorans. Extremophiles 6 (2002) 185-194
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
16. Lhadi E.K., Tazi H., Aylaj M., Genevini P.L., and Adani F. Organic matter evolution during co-composing of the organic fraction of municipal waste and poultry manure. Bioresour. Technol. 97 (2006) 2117-2123
17. Lin X., Wong S.L., Miller E.S., and Shih J.C.H. Expression of Bacillus lichniformis PWD-1 keratinase gene in B. subtilis. J. Ind. Microbiol. Biotechnol. 19 (1997) 134-138
18. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
19. Macedo A.J., Silva W.O.B.D., Gava R., Driemeier D., Henriques J.A.P., and Termignoni C. Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities. Appl. Environ. Microbiol. 71 (2005) 594-596
20. Malten M., Hollmann R., Deckwer W.D., and Jahn D. Production and Secretion of Recombinant Leuconostoc mesenteroides Dextransucrase DsrS in Bacillus megaterium. Biotechnol. Bioeng. 89 (2005) 206-218
21. Mallonee D.H., and Speckman R.A. Transformation of Bacillus polymyxa with plasmid DNA. Appl. Environ. Microbiol. 55 (1989) 2517-2521
22. McCool G.J., and Cannon M.C. PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium. J. Bacteriol. 183 (2001) 4235-4243
23. Muhsin T.M., and Hadi R.B. Degradation of keratin substrates by fungi isolated from sewage sludge. Mycopatholgia 154 (2001) 185-189
24. Nilegaonkar S.S., Zambare V.P., and Sarnaik P.P.K. Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326. Bioresour. Technol. 98 (2007) 1238-1245
25. Noronha E.F., Dolabela de Lima B., Martins de Sa C., and Felix E.F. Heterologous production of Aspergillus fumigatus keratinase in Pichia pastoris. World J. Microbiol. Biotechnol. 18 (2002) 563-568
26. Onifade A.A., Al-Sane N.A., Al-Musallam A.A., and Al-Zarban S. A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour. Technol. 66 (1998) 1-11
27. Pan J., Huang Q., and Zhang Y. Gene cloning and expression of an alkaline serine protease with dehairing function from Bacillus pumilus. Curr. Microbiol. 49 (2004) 165-169
28. Peng Y., Yang X.J., Xiao L., and Zhang Y.Z. Cloning and expression of a fibrinolytic enzyme (subtilisin DFE) gene from Bacillus amyloliquefaciens DC-4 in Bacillus subtilis. Res. Microbiol. 155 (2004) 167-173
29. Radha S., and Gunasekaran P. Cloning and expression of keratinase gene in Bacillus megaterium and optimization of fermentation condition for the production of kereatinase by recombinant strain. J. Appl. Microbiol. 103 (2007) 1301-1310
30. Ramani P., and Gupta R. Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RG1 using statistical methods involving response surface methodology. Biotechnol. Appl. Biochem. 40 (2004) 191-196
31. Riffel A., Lucas F., Heeb P., and Brandelli A. Characterization of a new keratinolytic bacterium that completely degrades native feather keratin. Arch. Microbiol. 179 (2003) 258-265
32. Rygus T., and Hillen W. Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon. Appl. Micobiol. Biotechnol. 35 (1991) 594-599
33. Sambrook, J., Fritsch, E. F., Maniatis, T., 1989. Molecular cloning. A Laboratory Manual, second ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
34. Sangali S., and Brandelli A. Feather keratin hydrolysis by a Vibrio sp. strain kr2. J. Appl. Microbiol. 89 (2000) 735-743
35. Scheler A., Rygus T., Allmansberger R., and Hillen W. Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization. Arch. Microbiol. 155 (1991) 526-534
36. Secades P., and Guijarro J.A. Purification and characterization of an extra-cellular protease from the fish pathogen Yersinia rucheri and effect of culture conditions on production. Appl. Environ. Microbiol. 65 (1999) 3969-3975
37. Suntornsuk W., and Suntornsuk L. Feather degradation by Bacillus sp. FK 46 in submerged cultivation. Bioresour. Technol. 86 (2003) 239-243
38. Suzuki Y., Tsujimoto Y., Matsui H., and Watanabe K. Decomposition of extremely hard-to-degrade animal proteins by thermphilic bacteria. J. Biosci. Bioeng. 102 (2006) 73-81
39. Talarico L.A., Gil M.A., Yomano L.P., Ingram L.O., and Furlow J.A.M. Construction and expression of an ethanol production operon in Gram- positive bacteria. Microbiol. 151 (2005) 4023-4031
40. Tobe S., Shimogaki H., Ohdera M., Asai Y., Oba K., Iwama M., and Irie M. Expression of Bacillus protease (Protease BYA) from Bacillus sp. Y in Bacillus subtilis and enhancement of its specific activity by site-directed mutagenesis- improvement in productivity of detergent enzyme. Biol. Pharm. Bull. 29 (2006) 26-33
41. Tran L., Wu X.C., and Wong S.L. Cloning and expression of a Novel protease gene encoding an extracellular neutral protease from Bacillus subtilis. J. Bacteriol. 173 (1991) 6364-6372
42. Wang J.J., Garlich J.D., and Shih J.C.H. Beneficial effects of versazyme, a keratinase feed additive on body weight, feed conversion and breast yield of broiler chickens. J. Appl. Poult. Res. 15 (2006) 544-550
43. Wang J.J., Rojanatavorn K., and Shih J.C.H. Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene. Biotechnol. Bioeng. 87 (2004) 459-464
44. Wang J.J., and Shih J.C.H. Fermentation production of keratinase from Bacillus lichniformis PWD-1 and recombinant B. subtilis FDB-29. J. Ind. Microbiol. Biotechnol. 22 (1999) 608-616
45. Wang J.J., Swaisgood H.E., and Shih J.C.H. Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems. Biotechnol. Bioeng. 81 (2003) 421-429
46. Williams C.M., Richter C.S., Mackenzie J.M., and Shih J.C.H. Isolation, Identification and characterization of a feather-degrading bacterium. Appl. Environ. Microbiol. 56 (1990) 1509-1515
47. Wittchen K.D., and Meinhardt F. Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl. Microbiol. Biotechnol. 42 (1995) 871-877
48. Xiao L., Zang R.H., Peng Y., and Zhang Y.Z. Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus subtilis mediated by the promoter of α-amylase gene from Bacillus amyloliquefaciens. Biotechnol. Letts. 26 (2004) 1365-1369