Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme

Protein Expression and Purification

Volumn 83, Issue 2, 2012, Pages 164-168

  Liu, Sen Lin ^a   Du, Kun ^a  

^a SHENZHEN UNIVERSITY   (China)

Author keywords

Bacillus subtilis; Bacterial endoglucanase; Inducible promoter; Overexpression

Indexed keywords

BACTERIAL PROTEIN; CELLULASE; CHELATING AGENT; METAL; RECOMBINANT PROTEIN; SUCROSE;

ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR CLONING; PH; PROMOTER REGION; TEMPERATURE;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CELLULASE; CHELATING AGENTS; CLONING, MOLECULAR; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; METALS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; SUCROSE; TEMPERATURE;

BACILLUS (BACTERIUM); BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 84860111270     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.03.015     Document Type: Article

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