Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein

Biotechnology Letters

Volumn 37, Issue 4, 2015, Pages 899-906

  Chen, Jingqi ^a,b,c   Gai, Yuanming ^b,c   Fu, Gang ^b,c,d   Zhou, Wenjuan ^b,c   Zhang, Dawei ^b,c,d   Wen, Jianping ^a  

^a TIANJIN UNIVERSITY   (China)

^b Chinese Academy of Sciences   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^d National Engineering Laboratory for Industrial Enzymes   (China)

Author keywords

Bacillus subtilis; Fed batch fermentation; Promoter; PrsA; Regulatory elements; Signal peptide; Amylase

Indexed keywords

BACTERIOLOGY; CELL PROLIFERATION; FERMENTATION; LIPOPROTEINS; PEPTIDES;

BACILLUS SUBTILIS; FED-BATCH FERMENTATION; PROMOTER; PRSA; REGULATORY ELEMENTS; SIGNAL PEPTIDE;

AMYLASES;

BACILLUS SUBTILIS;

AMYLASE; BACTERIAL PROTEIN; LIPOPROTEIN; MEMBRANE PROTEIN; PRSA PROTEIN, BACTERIA; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

BACILLUS SUBTILIS; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROMOTER REGION; SECRETION (PROCESS);

ALPHA-AMYLASES; BACILLUS SUBTILIS; BACTERIAL PROTEINS; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; LIPOPROTEINS; MEMBRANE PROTEINS; METABOLIC ENGINEERING; PROMOTER REGIONS, GENETIC; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS;

EID: 84925119596     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-014-1755-3     Document Type: Article

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