Highly efficient primed spacer acquisition from targets destroyed by the Escherichia coli type I-E CRISPR-Cas interfering complex

Proceedings of the National Academy of Sciences of the United States of America

Volumn 113, Issue 27, 2016, Pages 7626-7631

  Semenova, Ekaterina ^a   Savitskaya, Ekaterina ^b,c   Musharova, Olga ^b,d,e   Strotskaya, Alexandra ^a,b,c,e   Vorontsova, Daria ^a,b,e   Datsenko, Kirill A ^a,f   Logacheva, Maria D ^g   Severinov, Konstantin ^a,b,c,d,e  

^a RUTGERS UNIVERSITY   (United States)

^b SKOLKOVO INSTITUTE OF SCIENCE AND TECHNOLOGY   (Russian Federation)

^c INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

^d INSTITUTE OF GENE BIOLOGY   (Russian Federation)

^e PETER THE GREAT ST PETERSBURG POLYTECHNIC UNIVERSITY   (Russian Federation)

^f PURDUE UNIVERSITY   (United States)

^g MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Crispr interference; CRISPR Cas; Primed crispr adaptation

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; CRISPR ASSOCIATED PROTEIN; PLASMID DNA; PLASMID VECTOR; CAS2 PROTEIN, E COLI; DEOXYRIBONUCLEASE; ENDONUCLEASE; ESCHERICHIA COLI PROTEIN; SPACER DNA; YGBT PROTEIN, E COLI;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CELL GROWTH; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA DEGRADATION; DNA STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION; INTERACTIONS WITH RNA; MOLECULAR RECOGNITION; MOLECULE; MUTATION; NONHUMAN; PENICILLIN RESISTANCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTOSPACER; RNA SEQUENCE; RNA STRUCTURE; ADAPTATION; METABOLISM; PHYSIOLOGY;

ADAPTATION, BIOLOGICAL; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DNA, INTERGENIC; ENDODEOXYRIBONUCLEASES; ENDONUCLEASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS;

EID: 84977273804     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1602639113     Document Type: Article

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