Microaerobic conversion of xylose to ethanol in recombinant Saccharomyces cerevisiae SX6MUT expressing cofactor-balanced xylose metabolic enzymes and deficient in ALD6

Journal of Biotechnology

Volumn 227, Issue , 2016, Pages 72-78

  Jo, Sung Eun ^a   Seong, Yeong Je ^a   Lee, Hyun Soo ^a   Lee, Soo Min ^b   Kim, Soo Jung ^a,c   Park, Kyungmoon ^d   Park, Yong Cheol ^a  

^a Kookmin University   (South Korea)

^b Korea Forest Research Institute   (South Korea)

^c Seoul National University   (South Korea)

^d Hong Ik University   (South Korea)

Author keywords

ALD6; Ethanol; NADH preferring xylose reductase; Pitch pine hydrolysates; Saccharomyces cerevisiae; Xylose

Indexed keywords

COST EFFECTIVENESS; ENZYMES; METABOLISM; SUGAR SUBSTITUTES; XYLOSE; YEAST;

ALD6; ALDEHYDE DEHYDROGENASE; BATCH FERMENTATION; CELLULOSIC BIOMASS; ETHANOL PRODUCTIVITY; MICROAEROBIC CONDITIONS; PITCH PINE; XYLOSE REDUCTASE;

ETHANOL;

ALCOHOL; ALDEHYDE DEHYDROGENASE; ALDEHYDE DEHYDROGENASE 6; CELLULOSE; GLYCEROL; UNCLASSIFIED DRUG; XYLITOL; XYLOSE; ACETIC ACID DERIVATIVE; ALDEHYDE DEHYDROGENASE (NAD(P)+); COENZYME; RECOMBINANT PROTEIN;

ACTIVE TRANSPORT; AERATION; AEROBIC METABOLISM; ALCOHOL PRODUCTION; ARTICLE; BACTERIUM CULTURE; BATCH FERMENTATION; BIOTRANSFORMATION; CARBOHYDRATE METABOLISM; CARBON SOURCE; CELL GROWTH; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; FUNGAL BIOMASS; FUNGAL STRAIN; GLUCOSE INTAKE; NONHUMAN; PH; PINE; PINUS RIGIDA; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; STEADY STATE; SUGAR TRANSPORT; BATCH CELL CULTURE; CHEMISTRY; COENZYME; DRUG EFFECTS; FERMENTATION; GENETIC RECOMBINATION; GENETICS; HYDROLYSIS; METABOLISM;

ACETATES; AEROBIOSIS; ALDEHYDE OXIDOREDUCTASES; BATCH CELL CULTURE TECHNIQUES; CARBOHYDRATE METABOLISM; COENZYMES; ETHANOL; FERMENTATION; HYDROLYSIS; PINUS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; XYLOSE;

EID: 84963594778     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.04.005     Document Type: Article

References (30)

1. Du J., Li S., Zhao H. Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis. Mol. Biosyst. 2010, 6:2150-2156.
2. Eliasson A., Christensson C., Wahlbom C.F., Hahn-Hagerdal B. Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1XYL2, and XKS1 in mineral medium chemostat cultures. Appl. Environ. Microbiol. 2000, 66:3381-3386.
3. Farwick A., Bruder S., Schadeweg V., Oreb M., Boles E. Engineering of yeast hexose transporters to transport d-xylose without inhibition by d-glucose. Proc. Natl. Acad. Sci. U. S. A. 2014, 111:5159-5164.
4. Ha S.-J., Galazka J.M., Rin Kim S., Choi J.-H., Yang X., Seo J.-H., Louise Glass N., Cate J.H.D., Jin Y.-S. Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation. Proc. Natl. Acad. Sci. U. S. A. 2011, 108:504-509.
5. Hasunuma T., Sanda T., Yamada R., Yoshimura K., Ishii J., Kondo A. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Microb. Cell Fact. 2011, 10:2.
6. Ibraheem O., Ndimba B.K. Molecular adaptation mechanisms employed by ethanologenic bacteria in response to lignocellulose-derived inhibitory compounds. Int. J. Biol. Sci. 2013, 9:598-612.
7. Jin Y.-S., Alper H., Yang Y.-T., Stephanopoulos G. Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach. Appl. Environ. Microbiol. 2005, 71:8249-8256.
8. Katahira S., Ito M., Takema H., Fujita Y., Tanino T., Tanaka T., Fukuda H., Kondo A. Improvement of ethanol productivity during xylose and glucose co-fermentation by xylose-assimilating S. cerevisiae via expression of glucose transporter Sut1. Enzyme Microb. Technol. 2008, 43:115-119.
9. Kim S.R., Park Y.-C., Jin Y.-S., Seo J.-H. Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism. Biotechnol. Adv. 2013, 31:851-861.
10. Kim S.R., Skerker J.M., Kang W., Lesmana A., Wei N., Arkin A.P., Jin Y.-S. Rational and evolutionary engineering approaches uncover a small set of genetic changes efficient for rapid xylose fermentation in Saccharomyces cerevisiae. PLoS One 2013, 8:e57048.
11. Kotter P., Ciriacy M. Xylose fermentation by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 1993, 38:776-783.
12. Kuyper M., Toirkens M.J., Diderich J.A., Winkler A.A., van Dijken J.P., Pronk J.T. Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain. FEMS Yeast Res. 2005, 5:925-934.
13. Lee W.J., Kim M.D., Ryu Y.W., Bisson L.F., Seo J.H. Kinetic studies on glucose and xylose transport in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 2002, 60:186-191.
14. Lee S.-H., Kodaki T., Park Y.-C., Seo J.-H. Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae. J. Biotechnol. 2012, 158:184-191.
15. Lee J.S., Park E.H., Kim J.W., Yeo S.H., Kim M.D. Growth and fermentation characteristics of Saccharomyces cerevisiae NK28 isolated from kiwi fruit. J. Microbiol. Biotechnol. 2013, 23:1253-1259.
16. Lee H.-J., Kim S.-J., Yoon J.-J., Kim K.H., Seo J.-H., Park Y.-C. Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol. Bioresour. Technol. 2015, 191:445-451.
17. +-dependent xylitol dehydrogenase increases ethanol production from xylose in recombinant Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 2008, 81:243-255.
18. Olofsson K., Bertilsson M., Liden G. A short review on SSF-an interesting process option for ethanol production from lignocellulosic feedstocks. Biotechnol. Biofuels 2008, 1:7.
19. Petschacher B., Nidetzky B. Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae. Microb. Cell Fact. 2008, 7:9.
20. Shuler M.L., Kargi F. Bioprocess Engnieering Basic Concepts 2002, Prentice Hall, Inc., NJ, USA. 2nd ed.
21. Sonderegger M., Jeppsson M., Hahn-Hagerdal B., Sauer U. Molecular basis for anaerobic growth of Saccharomyces cerevisiae on xylose, investigated by global gene expression and metabolic flux analysis. Appl. Environ. Microbiol. 2004, 70:2307-2317.
22. Toivari M.H., Aristidou A., Ruohonen L., Penttilä M. Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae: importance of xylulokinase (XKS1) and oxygen availability. Metab. Eng. 2001, 3:236-249.
23. Van Uytvanck P.P., Hallmark B., Haire G., Marshall P.J., Dennis J.S. Impact of biomass on industry: using ethylene derived from bioethanol within the polyester value chain. ACS Sustain. Chem. Eng. 2014, 2:1098-1105.
24. Van Vleet J.H., Jeffries T.W. Yeast metabolic engineering for hemicellulosic ethanol production. Curr. Opin. Biotechnol. 2009, 20:300-306.
25. Van Vleet J.H., Jeffries T.W., Olsson L. Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces cerevisiae improves growth and ethanol production on d-xylose. Metab. Eng. 2008, 10:360-369.
26. Verho R., Londesborough J., Penttila M., Richard P. Engineering redox cofactor regeneration for improved pentose fermentation in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 2003, 69:5892-5897.
27. Walfridsson M., Anderlund M., Bao X., Hahn-Hagerdal B. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation. Appl. Microbiol. Biotechnol. 1997, 48:218-224.
28. +-dependent xylitol dehydrogenase. J. Biotechnol. 2007, 130:316-319.
29. Wei N., Quarterman J., Kim S.R., Cate J.H.D., Jin Y.-S. Enhanced biofuel production through coupled acetic acid and xylose consumption by engineered yeast. Nat. Commun. 2013, 4:2580.
30. Xiong M., Chen G., Barford J. Alteration of xylose reductase coenzyme preference to improve ethanol production by Saccharomyces cerevisiae from high xylose concentrations. Bioresour. Technol. 2011, 102:9206-9215.