Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Miyaoka, Yuichiro ^a   Berman, Jennifer R ^b   Cooper, Samantha B ^b   Mayerl, Steven J ^a   Chan, Amanda H ^a   Zhang, Bin ^b   Karlin Neumann, George A ^b,d   Conklin, Bruce R ^a,c  

^a GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

^b BIO RAD LABORATORIES   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE;

BIOASSAY; CELL LINE; CHEMISTRY; CRISPR CAS SYSTEM; CYTOLOGY; DNA END JOINING REPAIR; DOUBLE STRANDED DNA BREAK; GENE EDITING; GENE LOCUS; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; HELA CELL LINE; HUMAN; HUMAN GENOME; INDUCED PLURIPOTENT STEM CELL; METABOLISM; PLASMID; POLYMERASE CHAIN REACTION; RECOMBINATION REPAIR; SINGLE STRANDED DNA BREAK;

BIOLOGICAL ASSAY; CELL LINE; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; DNA BREAKS, SINGLE-STRANDED; DNA END-JOINING REPAIR; GENE EDITING; GENETIC LOCI; GENOME, HUMAN; HEK293 CELLS; HELA CELLS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINATIONAL DNA REPAIR; TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES; TRANSFECTION;

EID: 84962866438     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep23549     Document Type: Article

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