Isolation of single-base genome-edited human iPS cells without antibiotic selection

Nature Methods

Volumn 11, Issue 3, 2014, Pages 291-293

  Miyaoka, Yuichiro ^a   Chan, Amanda H ^a   Judge, Luke M ^a,b   Yoo, Jennie ^a   Huang, Miller ^b   Nguyen, Trieu D ^a   Lizarraga, Paweena P ^a   So, Po Lin ^a   Conklin, Bruce R ^a,b  

^a GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; GENOMIC DNA; NUCLEASE; OLIGONUCLEOTIDE;

AMPLICON; ANTIBIOTIC RESISTANCE; ARTICLE; CELL MUTANT; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA END JOINING REPAIR; DNA SEQUENCE; ENZYME METABOLISM; GENE DELETION; GENE FREQUENCY; GENE MUTATION; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN GENOME; MUTAGENESIS; NUCLEIC ACID BASE SUBSTITUTION; PLURIPOTENT STEM CELL; POINT MUTATION; PRIORITY JOURNAL; STRUCTURAL HOMOLOGY;

IPS;

ANTI-BACTERIAL AGENTS; BASE COMPOSITION; CELL LINE; CLONING, MOLECULAR; CYTOLOGICAL TECHNIQUES; GENOME, HUMAN; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MUTATION;

EID: 84895528015     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.2840     Document Type: Article

References (30)

1. da Cunha Santos, G., Shepherd, F.A. & Tsao, M.S. Annu. Rev. Pathol. 6, 49-69 (2011).
2. Moore, J.R., Leinwand, L. & Warshaw, D.M. Circ. Res. 111, 375-385 (2012).
3. Takahashi, K. et al. Cell 131, 861-872 (2007).
4. Yu, J. et al. Science 318, 1917-1920 (2007).
5. Gaj, T., Gersbach, C.A. & Barbas, C.F. III. Trends Biotechnol. 31, 397-405 (2013).
6. Fu, Y. et al. Nat. Biotechnol. 31, 822-826 (2013).
7. Gupta, A., Meng, X., Zhu, L.J., Lawson, N.D. & Wolfe, S.A. Nucleic Acids Res. 39, 381-392 (2011).
8. Hsu, P.D. et al. Nat. Biotechnol. 31, 827-832 (2013).
9. Pattanayak, V., Ramirez, C.L., Joung, J.K. & Liu, D.R. Nat. Methods 8, 765-770 (2011).
10. Ding, Q. et al. Cell Stem Cell 12, 238-251 (2013).
11. Chen, F. et al. Nat. Methods 8, 753-755 (2011).
12. Soldner, F. et al. Cell 146, 318-331 (2011).
13. Mali, P. et al. Nat. Biotechnol. 31, 833-838 (2013).
14. Ran, F.A. et al. Cell 154, 1380-1389 (2013).
15. McCormick, M. Methods Enzymol. 151, 445-449 (1987).
16. Hindson, B.J. et al. Anal. Chem. 83, 8604-8610 (2011).
17. Cong, L. et al. Science 339, 819-823 (2013).
18. Mali, P. et al. Science 339, 823-826 (2013).
19. Yusa, K. et al. Nature 478, 391-394 (2011).
20. Doyle, E.L. et al. Nucleic Acids Res. 40, W117-W122 (2012).
21. Sander, J.D. et al. Nucleic Acids Res. 38, W462-W468 (2010).
22. Sander, J.D., Zaback, P., Joung, J.K., Voytas, D.F. & Dobbs, D. Nucleic Acids Res. 35, W599-W605 (2007).
23. Cermak, T. et al. Nucleic Acids Res. 39, e82 (2011).
24. Bedell, V.M. et al. Nature 491, 114-118 (2012).
25. Christian, M.L. et al. PLoS ONE 7, e45383 (2012).
26. Cong, L., Zhou, R., Kuo, Y.C., Cunniff, M. & Zhang, F. Nature Commun. 3, 968 (2012).
27. Kreitzer, F.R. et al. Am. Journal Stem Cells 2, 119-131 (2013).
28. Okita, K. et al. Nat. Methods 8, 409-412 (2011).
29. Watanabe, K. et al. Nat. Biotechnol. 25, 681-686 (2007).
30. Grau, J., Boch, J. & Posch, S. Bioinformatics 29, 2931-2932 (2013).