Impact of date palm fruits extracts and probiotic enriched diet on antioxidant status, innate immune response and immune-related gene expression of European seabass (Dicentrarchus labrax)

Fish and Shellfish Immunology

Volumn 52, Issue , 2016, Pages 298-308

  Guardiola, F A ^a   Porcino, C ^b   Cerezuela, R ^a   Cuesta, A ^a   Faggio, C ^b   Esteban, M A ^a  

^a UNIVERSITY OF MURCIA   (Spain)

^b UNIVERSITY OF MESSINA   (Italy)

Author keywords

Antioxidant status; Date palm fruit extracts; European sea bass (Dicentrarchus labrax); Immunostimulants; Innate immunity; Probiotics; Teleosts

Indexed keywords

ANTIOXIDANT; PLANT EXTRACT; PROBIOTIC AGENT;

ANALYSIS; ANIMAL; ANIMAL FOOD; BASS; CHEMISTRY; DIET; DRUG EFFECTS; FRUIT; GENE EXPRESSION REGULATION; IMMUNOLOGY; INNATE IMMUNITY; METABOLISM; MICROBIOLOGY; PHOENIX (PLANT); PHYSIOLOGY; RANDOMIZATION; SHEWANELLA PUTREFACIENS; VETERINARY;

ANIMAL FEED; ANIMALS; ANTIOXIDANTS; BASS; DIET; FRUIT; GENE EXPRESSION REGULATION; IMMUNITY, INNATE; PHOENICEAE; PLANT EXTRACTS; PROBIOTICS; RANDOM ALLOCATION; SHEWANELLA PUTREFACIENS;

EID: 84962038972     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2016.03.152     Document Type: Article

References (99)

1. FAO, Fisheries and Aquaculture topics The State of World Fisheries and Aquaculture (SOFIA) 2014, Topics fact sheets, FAO Fish. Aquac. Dep., Rome.
2. R. Subasinghe. Aquaculture development: the blue revolution, Stock. R. Swedish Acad. Agric. For. (2009) 281-302.
3. Lazado C.C., Marlowe C., Caipang A., Estante E.G. Prospects of host-associated microorganisms in fish and penaeids as probiotics with immunomodulatory functions. Fish. Shellfish Immunol. 2015, 45:2-12. 10.1016/j.fsi.2015.02.023.
4. Amar C., Kiron V., Satoh S., Okamoto N., Watabane T. Effects of dietary β- carotene on the immune response of rainbow trout Oncorhynchus mykiss. Fish. Sci. 2000, 66:1068-1075.
5. Raa J., Roerstad G. The use of immunostimulants to increase resistance of aquatic organisms to microbial infections. Diseases in Asian Aquaculture Vol. 1. Fish Health Section 1992, 39-50. Asian Fisheries Society, Manila, Philippines. M. Shariff, R.P. Subasighe, J. Arthur (Eds.).
6. Secombes C. Enhancement of fish phagocyte activity. Fish. Shellfish Immunol. 1994, 4:421-436.
7. Magnadottir B. Immunological control of fish diseases. Mar. Biotechnol. N. Y. 2010, 12:361-379.
8. Sakai M. Current research status of fish immunostimulants. Aquaculture 1999, 172:63-92. 10.1016/S0044-8486(98)00436-0.
9. Nayak S.K. Probiotics and immunity: a fish perspective. Fish. Shellfish Immunol. 2010, 29:2-14. 10.1016/j.fsi.2010.02.017.
10. R. Cerezuela, J. Meseguer, M. A. Esteban. Current knowledge in synbiotic use for fish aquaculture: A Review, (2011) 1-7. doi:10.4172/2155-9546.S1-008.
11. Song S.K., Beck B.R., Kim D., Park J., Kim J., Kim H.D., et al. Prebiotics as immunostimulants in aquaculture: A review. Fish. Shellfish Immunol. 2014, 40:40-48. 10.1016/j.fsi.2014.06.016.
12. Reverter M., Bontemps N., Lecchini D., Banaigs B., Sasal P. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture 2014, 433:50-61. 10.1016/j.aquaculture.2014.05.048.
13. Chakraborty S.B., Hancz C. Application of phytochemicals as immunostimulant, antipathogenic and antistress agents in finfish culture. Rev. Aquac. 2011, 3:103-119. 10.1111/j.1753-5131.2011.01048.x.
14. Citarasu T. Herbal biomedicines: a new opportunity for aquaculture industry. Aquac. Int. 2010, 18:403-414. 10.1007/s10499-009-9253-7.
15. Harikrishnan R., Balasundaram C., Heo M.S. Diet enriched with mushroom Phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, Epinephelus bruneus against vibriosis. Fish. Shellfish Immunol. 2011, 30:128-134. 10.1016/j.fsi.2010.09.013.
16. Logambal S.M., Venkatalakshmi S., Michael R.D. Immunostimulatory effect of leaf extract of Ocimum sanctum Linn. in Oreochromis mossambicus (Peters). Hydrobiologia 2000, 430:113-120. 10.1023/A:1004029332114.
17. Olusola S.E., Emikpe B.O., Olaifa F.E. The potentials of medicinal plant extracts as bio-antimicrobials in aquaculture. Int. J. Med. Aromat. Plants 2013, 3:404-412.
18. Cerezuela R., Guardiola F.A., Cuesta A., Esteban M.Á. Enrichment of gilthead seabream (Sparus aurata L.) diet with palm fruit extracts and probiotics: Effects on skin mucosal immunity. Fish. Shellfish Immunol. 2016, 49:100-109.
19. Tang Z.X., Shi L.E., Aleid S.M. Date fruit: Chemical composition, nutritional and medicinal values, products. J. Sci. Food Agric. 2013, 93:2351-2361.
20. Esteban M.A., Cordero H., Martínez-Tomé M., Jiménez-Monreal A., Bakhrouf A., Mahdhi A. Effect of dietary supplementation of probiotics and palm fruits extracts on the antioxidant enzyme gene expression in the mucosae of gilthead seabream (Sparus aurata L.). Fish. Shellfish Immunol. 2014, 39:532-540. 10.1016/j.fsi.2014.06.012.
21. Sarmadi B.H., Ismail A. Antioxidative peptides from food proteins: A review. Peptides 2010, 31:1949-1956. 10.1016/j.peptides.2010.06.020.
22. Najafian L., Babji A.S. A review of fish-derived antioxidant and antimicrobial peptides: Their production, assessment, and applications. Peptides 2012, 33:178-185. 10.1016/j.peptides.2011.11.013.
23. Chaira N., Smaali M.I., Martinez-Tomé M., Mrabet A., Murcia M.A., Ferchichi A. Simple phenolic composition, flavonoid contents and antioxidant capacities in water-methanol extracts of Tunisian common date cultivars (Phoenix dactylifera L.). Int. J. Food Sci. Nutr. 2009, 60(Suppl. 7):316-329.
24. Mansouri A., Embarek G., Kokkalou E., Kefalas P. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chem. 2005, 89:411-420.
25. Balcázar J.L., de Blas I., Ruiz-Zarzuela I., Cunningham D., Vendrell D., Mázquiz J.L. The role of probiotics in aquaculture. Vet. Microbiol. 2006, 114:173-186.
26. Wang Y.B., Li J.R., Lin J. Probiotics in aquaculture: Challenges and outlook. Aquaculture 2008, 281:1-4. 10.1016/j.aquaculture.2008.06.002.
27. Díaz-Rosales P., Salinas I., Rodríguez A., Cuesta A., Chabrillón M., Balebona M.C., et al. Gilthead seabream (Sparus aurata L.) innate immune response after dietary administration of heat-inactivated potential probiotics. Fish. Shellfish Immunol. 2006, 20:482-492. 10.1016/j.fsi.2005.06.007.
28. Tapia-Paniagua S.T., Díaz-Rosales P., León-Rubio J.M., García de La Banda I., Lobo C., Alarcón F.J., et al. Use of the probiotic Shewanella putrefaciens Pdp11 on the culture of Senegalese sole (Solea senegalensis, Kaup 1858) and gilthead seabream (Sparus aurata L.). Aquac. Int. 2012, 20:1025-1039. 10.1007/s10499-012-9509-5.
29. Mahdhi A., Bahi A., Mzah D., Bakhrouf A. Use of mixture design to construct a consortium of date palm (Phoenix dactylifera L.) fruit extract and potentially probiotic Bacillus strain to confer protection against vibriosis in Artemia culture. J. Sci. Food Agric. 2013, 93:3850-3855.
30. Díaz-Rosales P., Salinas I., Rodríguez A., Cuesta A., Chabrillón M., Balebona M.C., et al. Gilthead seabream (Sparus aurata L.) innate immune response after dietary administration of heat-inactivated potential probiotics. Fish. Shellfish Immunol. 2006, 20:482-492. 10.1016/j.fsi.2005.06.007.
31. Esteban M., Mulero V., Muñoz J., Meseguer J. Methodological aspects of assessing phagocytosis of Vibrio anguillarum by leucocytes of gilthead seabream (Sparus aurata L.) by flow cytometry and electron microscopy. Cell Tissue Res. 1998, 293:133-141.
32. Sunyer J.O., Tort L. Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are effected by the alternative complement pathway. Vet. Immunol. Immunopathol. 1995, 45:333-345.
33. Ross N.W., Firth K.J., Wang A., Burka J.F., Johnson S.C. Changes in hydrolytic enzyme activities of naïve Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation. Dis. Aquat. Organ 2000, 41:43-51. 10.3354/dao041043.
34. Hanif A., Bakopoulos V., Dimitriadis G.J. Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae. Fish. Shellfish Immunol. 2004, 17:411-435. 10.1016/j.fsi.2004.04.013.
35. Ortuño J., Esteban M.Á., Mulero V., Meseguer J. Methods for studying the haemolytic, chemoattractant and opsonic activities of seabream (Sparus aurata L.). Methodol. Fish Dis. Res. 1998, 97-100. Albion Press. A.C. Barnes, G.A. Davidson, M. Hiney, D. McInthos (Eds.).
36. Quade M.J., Roth J.A. A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet. Immunol. Immunopathol. 1997, 58:239-248.
37. Cuesta A., Meseguer J., Esteban M.A. Total serum immunoglobulin M levels are affected by immunomodulators in seabream (Sparus aurata L.). Vet. Immunol. Immunopathol. 2004, 101:203-210. 10.1016/j.vetimm.2004.04.021.
38. Bayne C.J., Levy S. Modulation of the oxidative burst in trout myeloid cells by adrenocorticotropic hormone and catecholamines: mechanisms of action. J. Leukoc. Biol. 1991, 50:554-560.
39. Rodríguez A., Esteban M.A., Meseguer J. Phagocytosis and peroxidase release by seabream (Sparus aurata L.) leucocytes in response to yeast cells. Anat. Rec. A. Discov. Mol. Cell. Evol. Biol. 2003, 272:415-423. 10.1002/ar.a.10048.
40. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 1993, 15:532-537.
41. Harikrishnan R., Kim M., Kim J., Balasundaram C., Heo M. Protective effect of herbal and probiotics enriched diet on haematological and immunity status of Oplegnathus fasciatus (Temminck & Schlegel) against Edwardsiella tarda. Fish. Shellfish Immunol. 2011, 30:886-893. 10.1016/j.fsi.2011.01.013.
42. Akhter N., Wu B., Memon A.M., Mohsin M. Probiotics and prebiotics associated with aquaculture. A Review. Fish. Shellfish Immunol. 2015, 45:733-741. 10.1016/j.fsi.2015.05.038.
43. Galina J., Yin G., Ardó L., Jeney Z. The use of immunostimulating herbs in fish. An overview of research. Fish. Physiol. Biochem. 2009, 35:669-676. 10.1007/s10695-009-9304-z.
44. Vaseeharan B., Thaya R. Medicinal plant derivatives as immunostimulants: an alternative to chemotherapeutics and antibiotics in aquaculture. Aquac. Int. 2014, 22:1079-1091.
45. Hoseinifar S.H., Khalili M., Rufchaei R., Raeisi M. Effects of date palm fruit extracts on skin mucosal immunity, immune related genes expression and growth performance of common carp (Cyprinus carpio) fry. Fish. Shellfish Immunol. 2015, 47:706-711. 10.1016/j.fsi.2015.09.046.
46. Tapia-Paniagua S.T., Díaz-Rosales P., García de la Banda I., Lobo C., Clavijo E., Balebona M.C., et al. Modulation of certain liver fatty acids in Solea senegalensis is influenced by the dietary administration of probiotic microorganisms. Aquaculture 2014, 424-425:234-238. 10.1016/j.aquaculture.2014.01.003.
47. Tapia-paniagua S.T., Vidal S., Lobo C., García-de la Banda I., Esteban M.A., Balebona M.C., et al. Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline 2015, 46:449-458. 10.1016/j.fsi.2015.07.007.
48. Cordero H., Guardiola F.A., Tapia-Paniagua S.T., Cuesta A., Meseguer J., Balebona M.C., et al. Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.). Fish. Shellfish Immunol. 2015, 45:608-618. 10.1016/j.fsi.2015.05.010.
49. Poljsak B. Strategies for Reducing or Preventing the Generation of Oxidative Stress 2011, 2011:1-15. 10.1155/2011/194586.
50. Evans H.M., Bishop K.S. On the existence of a hitherto unrecognized dietary factor essential for reproduction. Science 1922, 56:650-651. 10.1126/science.56.1458.650.
51. Traber M.G., Stevens J.F. Vitamins C and E: beneficial effects from a mechanistic perspective. Free Radic. Biol. Med. 2011, 51:1000-1013. 10.1016/j.freeradbiomed.2011.05.017.
52. Lundebye A., Hove H., Måge A., Bohne V.J.B., Hamre K. Levels of synthetic antioxidants (ethoxyquin, butylated hydroxytoluene and butylated hydroxyanisole) in fish feed and commercially farmed fish. Food Addit. Contam-PART A Chem. Anal. Control. Expo. Risk Assess. 2010, 27:1652-1657. 10.1080/19440049.2010.508195.
53. Mehana E.E., Rahmani A.H., Aly S.M. Immunostimulants and fish culture: An overview. Annu. Res. Rev. Biol 2015, 5(6):477-489.
54. Alexander J.B., Ingram G.A. Noncellular nonspecific defence mechanisms of fish. Annu. Rev. Fish. Dis. 1992, 2:249-279. 10.1016/0959-8030(92)90066-7.
55. Kaleeswaran B., Ilavenil S., Ravikumar S. Screening of phytochemical properties and antibacterial activity of Cynodon dactylon L. Int. J. Curr. Res. 2010, 3:83-88.
56. Magnadóttir B., Jónsdóttir H., Helgason S., Björnsson B., Solem S.T., Pilström L. Immune parameters of immunised cod (Gadus morhua L.). Fish. Shellfish Immunol. 2001, 11:75-89. 10.1006/fsim.2000.0296.
57. Tremacoldi C., Pascholati S. Detection of trypsin inhibitor in seeds of Eucalyptus urophylla and its influence on the in vitro growth of the fungi Pisolithus tinctorius and Rhizoctonia solani. Braz. J. Microbiol. 2002, 33:281-286.
58. Nya E.J., Austin B. Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish. Dis. 2009, 32:963-970. 10.1111/j.1365-2761.2009.01100.x.
59. Nya E.J., Austin B. Use of dietary ginger, Zingiber officinale Roscoe, as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis. 2009, 32:971-977. 10.1111/j.1365-2761.2009.01101.x.
60. Talpur A.D., Ikhwanuddin M. Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch). Aquaculture 2012, 364-365:6-12. 10.1016/j.aquaculture.2012.07.035.
61. Talpur A.D., Ikhwanuddin M., Bolong A., Abol-Munafi M., Alpur A.D. Nutritional effects of ginger (Zingiber officinale Roscoe) on immune response of Asian sea bass, Lates calcarifer (Bloch) and disease resistance against Vibrio harveyi. Aquaculture 2013, 400-401:46-52. 10.1016/j.aquaculture.2013.02.043.
62. Talpur A.D., Ikhwanuddin M. Azadirachta indica (neem) leaf dietary effects on the immunity response and disease resistance of Asian seabass, Lates calcarifer challenged with Vibrio harveyi. Fish. Shellfish Immunol. 2013, 34:254-264. 10.1016/j.fsi.2012.11.003.
63. Talpur A.D. Mentha piperita (Peppermint) as feed additive enhanced growth performance, survival, immune response and disease resistance of Asian seabass, Lates calcarifer (Bloch) against Vibrio harveyi infection. Aquaculture 2014, 420-1:71-78. 10.1016/j.aquaculture.2013.10.039.
64. Sharifuzzaman S.M., Austin B. Influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout. Fish. Shellfish Immunol. 2009, 27:440-445. 10.1016/j.fsi.2009.06.010.
65. Harikrishnan R., Balasundaram C., Heo M. Potential use of probiotic- and triherbal extract-enriched diets to control Aeromonas hydrophila infection in carp 2010, 92:41-49. 10.3354/dao02240.
66. Harikrishnan R., Balasundaram C., Heo M.-S. Scuticociliatosis and its recent prophylactic measures in aquaculture with special reference to South Korea Taxonomy, diversity and diagnosis of scuticociliatosis: Part I Control strategies of scuticociliatosis: Part II. Fish. Shellfish Immunol. 2010, 29:15-31. 10.1016/j.fsi.2010.02.026.
67. Tang H.G., Wu T.X., Zhao Z.Y., Pan X.D. Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.). J. Zhejiang Univ. 2008, 9:684-690. 10.1631/jzus.B0820088.
68. Edahiro T., Hamoguchi M., Kusuda R. Suppressive effect of glycyrrhizin against streptococcal infection promoted by feeding oxidized lipids to yellowtail, Seriola quinqueradiata. Suisanzoshoku 1991, 39:21-27.
69. Park K.H., Choi S.H. The effect of mistletoe, Viscum album coloratum, extract on innate immune response of Nile tilapia (Oreochromis niloticus). Fish. Shellfish Immunol. 2012, 32:1016-1021. 10.1016/j.fsi.2012.02.023.
70. C. J. W. Kirubakaran, C. P. Alexander, R. D. Michael. Enhancement of non-specific immune responses and disease resistance on oral administration of Nyctanthes arbortristis seed extract in Oreochromis mossambicus (Peters), (2010) 1630-39. doi:10.1111/j.1365-2109.2010.02516.x.
71. Alexander C.P., Wesly C.J., Michael R.D. Water soluble fraction of Tinospora cordifolia leaves enhanced the non-specific immune mechanisms and disease resistance in Oreochromis mossambicus. Fish. Shellfish Immunol. 2010, 29:765-772. 10.1016/j.fsi.2010.07.003.
72. Harikrishnan R., Balasundaram C., Kim M.C., Kim J.S., Han Y.J., Heo M.S. Effect of a mixed herb-enriched diet on the innate immune response and disease resistance of Paralichthys olivaceus against Philasterides dicentrarchi infection. J. Aquat. Anim. Health 2010, 22:235-243. 10.1577/H09-040.1.
73. Divyagnaneswari M., Christybapita D., Michael R.D. Enhancement of nonspecific immunity and disease resistance in Oreochromis mossambicus by Solanum trilobatum leaf fractions. Fish. Shellfish Immunol. 2007, 23:249-259. 10.1016/j.fsi.2006.09.015.
74. Díaz-Rosales P., Arijo S., Chabrillón M., Alarcón F.J., Tapia-Paniagua S.T., Martínez-Manzanares E., et al. Effects of two closely related probiotics on respiratory burst activity of Senegalese sole (Solea senegalensis, Kaup) phagocytes, and protection against Photobacterium damselae subsp. piscicida. Aquaculture 2009, 293:16-21. 10.1016/j.aquaculture.2009.03.050.
75. Salinas I., Díaz-Rosales P., Cuesta A., Meseguer J., Chabrillón M., Moriñigo M.A., et al. Effect of heat-inactivated fish and non-fish derived probiotics on the innate immune parameters of a teleost fish (Sparus aurata L.). Vet. Immunol. Immunopathol. 2006, 111:279-286. 10.1016/j.vetimm.2006.01.020.
76. Yin G., Jeney G., Racz T., Xu P., Jun X., Jeney Z. Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus. Aquaculture 2006, 253:39-47.
77. Kim K., Jang S., Marsden M., Secombes C., Choi M., Kim Y., et al. Effect of glycyrrhizin on rainbow trout Oncorhynchus mykiss leukocyte responses. J. Korean Soc. Microbiol. 1998, 33:263-271.
78. Dügenci S.K., Arda N., Candan A. Some medicinal plants as immunostimulant for fish. J. Ethnopharmacol. 2003, 88:99-106. 10.1016/S0378-8741(03)00182-X.
79. Jian J.C., Wu Z.H. Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture 2003, 218:1-9. 10.1016/S0044-8486(02)00192-8.
80. Jian J., Wu Z. Influences of traditional Chinese medicine on non-specific immunity of Jian Carp (Cyprinus carpio var. Jian). Fish. Shellfish Immunol. 2004, 16:185-191. 10.1016/S1050-4648(03)00062-7.
81. Wu Y.R., Gong Q.F., Fang H., Liang W.W., Chen M., He R.J. Effect of Sophora flavescens on non-specific immune response of tilapia (GIFT Oreochromis niloticus) and disease resistance against Streptococcus agalactiae. Fish. Shellfish Immunol. 2013, 34:220-227. 10.1016/j.fsi.2012.10.020.
82. Dey R., Chandra S. Preliminary studies to raise disease resistant seed (fry) of Indian major carp Catla catla (Ham.) through herbal treatment of spawn. Fish. Chimes 1995, 14:23-25.
83. Wu C., Liu C., Chang Y., Hsieh S. Effects of hot-water extract of Toona sinensis on immune response and resistance to Aeromonas hydrophila in Oreochromis mossambicus. Fish. Shellfish Immunol. 2010, 29:258-263. 10.1016/j.fsi.2010.04.021.
84. Balaji S., Balasubramanian V., Baskaran S., Pavaraj M., Thangapandian V. Evaluation of immunomodulatory effect of dietary probiotics on the common carp, Cyprinus carpio. Res. J. Immunol. 2013, 6:1-6.
85. Oskoii S., Kohyani A., Parseh A., Salati A., Sadeghi E. Effects of dietary administration of Echinacea purpurea on growth indices and biochemical and hematological indices in rainbow trout (Oncorhynchus mykiss) fingerlings. Fish. Physiol. Biochem. 2012, 38:1029-1034.
86. B. S. Sahu, B. K. Das, B. K. Mishra, J. Pradhan, N. Sarangi. Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila, 23 (2007) 80-6. doi:10.1111/j.1439-0426.2006.00785.x.
87. Sivaram V., Babu M., Immanuel G., Murugadass S., Citarasu T., Marian M. Growth and immune response of juvenile greasy groupers (Epinephelus tauvina) fed with herbal antibacterial active principle supplemented diets against Vibrio harveyi infections. Aquaculture 2004, 237:9-20.
88. Yuan C., Li D., Chen W., Sun F., Wu G., Gong Y., et al. Administration of a herbal immunoregulation mixture enhances some immune parameters in carp (Cyprinus carpio) 2007, 93-101. 10.1007/s10695-006-9120-7.
89. R. Harikrishnan, C. Balasundaram, M. Heo, Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish, 317 (2011) 1-15. doi:10.1016/j.aquaculture.2011.03.039.
90. R. Harikrishnan, C. Balasundaram, M. Kim, Y. Han, M. Heo, Effect of a mixed herb-enriched diet on the innate immune response and disease resistance of Paralichthys olivaceus against Philasterides dicentrarchi infection, 22 (2011) 235-43. doi:10.1577/H09-040.1.
91. Rao Y.V., Das B.K., Jyotyrmayee P., Chakrabarti R. Effect of Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Fish. Shellfish Immunol. 2006, 20:263-273. 10.1016/j.fsi.2005.04.006.
92. Aly S.M., Abdel-Galil Ahmed Y., Abdel-Aziz Ghareeb A., Mohamed M.F. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish. Shellfish Immunol. 2008, 25:128-136. 10.1016/j.fsi.2008.03.013.
93. Kumar R., Mukherjee S.C., Ranjan R., Nayak S.K. Enhanced innate immune parameters in Labeo rohita (Ham.) following oral administration of Bacillus subtilis. Fish. Shellfish Immunol. 2008, 24:168-172.
94. Cammarata M., Parisi M.G., Benenati G., Vasta G.R., Parrinello N. A rhamnose-binding lectin from sea bass (Dicentrarchus labrax) plasma agglutinates and opsonizes pathogenic bacteria. Dev. Comp. Immunol. 2014, 44:332-340. 10.1016/j.dci.2014.01.019.
95. Kim D.H., Austin B. Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish. Shellfish Immunol. 2006, 21:513-524. 10.1016/j.fsi.2006.02.007.
96. Hsieh J.C., Pan C.Y., Chen J.Y. Tilapia hepcidin (TH)2-3 as a transgene in transgenic fish enhances resistance to Vibrio vulnificus infection and causes variations in immune-related genes after infection by different bacterial species. Fish. Shellfish Immunol. 2010, 29:430-439. 10.1016/j.fsi.2010.05.001.
97. Vaughan M. Oxidative modification of macromolecules minireview series. J. Biol. Chem. 1997, 272:18513.
98. Cammarata M., Vazzana M., Chinnici C. A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax. Biochim. Biophys. Acta 2001, 1528:196-202.
99. Li X.J., Kong D.X., Zhang H.Y. Chemoinformatics approaches for traditional Chinese medicine research and case application in anticancer drug discovery. Curr. Drug Discov. Technol. 2010, 7:22-31.