Characterization of aspartate kinase and homoserine dehydrogenase from Corynebacterium glutamicum IWJ001 and systematic investigation of l-isoleucine biosynthesis

Journal of Industrial Microbiology and Biotechnology

Volumn 43, Issue 6, 2016, Pages 873-885

  Dong, Xunyan ^a   Zhao, Yue ^a   Zhao, Jianxun ^a   Wang, Xiaoyuan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Aspartate kinase; Corynebacterium glutamicum; Feed back inhibition; Homoserine dehydrogenase; l Isoleucine biosynthesis; Metabolic engineering

Indexed keywords

ASPARTATE KINASE; CARBON; HOMOSERINE; HOMOSERINE DEHYDROGENASE; ISOLEUCINE; LYSINE; THREONINE; AMINO ACID; BACTERIAL PROTEIN;

ARTICLE; BIOSYNTHESIS; CORYNEBACTERIUM GLUTAMICUM; IC50; IN VITRO STUDY; NONHUMAN; CHEMISTRY; CULTURE MEDIUM; DNA FRAGMENTATION; ENZYMOLOGY; FERMENTATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; MICROBIOLOGY;

AMINO ACIDS; ASPARTATE KINASE; BACTERIAL PROTEINS; CORYNEBACTERIUM GLUTAMICUM; CULTURE MEDIA; DNA FRAGMENTATION; FERMENTATION; HOMOSERINE DEHYDROGENASE; INDUSTRIAL MICROBIOLOGY; ISOLEUCINE; LYSINE; METABOLIC ENGINEERING; THREONINE;

EID: 84962024803     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-016-1763-5     Document Type: Article

References (50)

1. Archer JAC, Solow-Cordero DE, Sinskey AJ (1991) A C-terminal deletion in Corynebacterium glutumicum homoserine dehydrogenase abolishes allosteric inhibition by l-threonine. Gene 107:53–59
2. Becker J, Zelder O, Häfner S, Schröder H, Wittmann C (2011) From zero to hero—Design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production. Metab Eng 13:159–168
3. Bertani G (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol 62:293–300
4. Black S, Wright NG (1954) β-aspartate kinase and β-aspartyl-phosphate. J Biol Chem 213:27–38
5. Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ (2008) Corynebacterium glutamicum tailored for high-yield l-valine production. Appl Microbiol Biotechnol 79:471–479
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
7. Chassagnole C, Raїs B, Quentin E, Fell DA, Mazat J (2001) An integrated study of threonine-pathway enzyme kinetics in Escherichia coli. Biochem J 356:415–423
8. Chen Z, Meyer W, Rappert S, Sun J, Zeng AP (2011) Co-evolutionary analysis enabled rational deregulation of allosteric enzyme inhibition in Corynebacterium glutamicum for lysine production. Appl Environ Microbiol 77:4352–4360
9. Chen Z, Rappert S, Zeng AP (2015) Rational design of allosteric regulation of homoserine dehydrogenase by a nonnatural inhibitor l-lysine. ACS Synth Biol 4:126–131
10. Colón GE, Nguyen TT, Jetten MSM, Sinskey AJ, Stephanopoulos G (1995) Production of l-isoleucine by overexpression of ilvA in a Corynebacterium lactofermentum threonine producer. Appl Microbiol Biotechnol 43:482–488
11. de Jong BW, Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J (2015) Metabolic pathway engineering for fatty acid ethyl ester production in Saccharomyces cerevisiae using stable chromosomal integration. J Ind Microbiol Biotechnol 42:477–486
12. Diesveld R, Tietze N, Fürst O, Reth A, Bathe B, Sahm H, Eggeling L (2009) Activity of exporters of Escherichia coli in Corynebacterium glutamicum, and their use to increase l-threonine production. J Mol Microbiol Biotechnol 16:198–207
13. Dong X, Quinn PJ, Wang X (2011) Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the production of l-threonine. Biotechnol Adv 29:11–23
14. Du J, Shao Z, Zhao H (2011) Engineering microbial factories for synthesis of value-added products. J Ind Microbiol Biotechnol 38:873–890
15. Eggeling I, Cordes C, Eggeling L, Sahm H (1987) Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of α-ketobutyrate to l-isoleucine. Appl Microbiol Biotechnol 25:346–351
16. Eggeling L, Bott M (2005) Handbook of Corybacterium glutamicum. Taylor & Francis, Boca Raton
17. Eggeling L, Bott M (2015) A giant market and a powerful metabolism: l-lysine provided by Corybacterium glutamicum. Appl Microbiol Biotechnol 99:3387–3394
18. Follettie MT, Shin HK, Sinskey AJ (1988) Organization and regulation of the Corynebacterium glutamicum hom-thrB and thrC loci. Mol Microbiol 2:53–62
19. Guillouet S, Rodal AA, An G, Lessard PA, Sinskey AJ (1999) Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production. Appl Environ Microbiol 65:3100–3107
20. Hasegawa S, Uematsu K, Natsuma Y, Suda M, Hiraga K, Jojima T, Inui M, Yukawa H (2012) Improvement of the redox balance increases l-valine production by Corynebacterium glutamicum under oxygen deprivation conditions. Appl Environ Microbiol 78(3):865–875
21. Hashiguchi K, Takesada H, Suzuki E, Matsui H (1999) Construction of an l-isoleucine overproducing strain of Escherichia coli K-12. Biosci Biotechnol Biochem 63:672–679
22. Holátko J, Elišáková V, Prouza M, Sobotka M, Nešvera J, Pátek M (2009) Metabolic engineering of the l-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation. J Biotechnol 139:203–210
23. James CL, Viola RE (2002) Production and characterization of bifunctional enzymes. Domain swapping to produce new bifunctional enzymes in the aspartate pathway. Biochemistry 41:3720–3725
24. Koros A, Varga ZS, Molnar P (2008) Simultaneous analysis of amino acids and amines as their ophthalaldehyde-ethanethiol-9-fluorenylmethyl chloroformate derivatives in cheese by high-performance liquid chromatography. J Chromatogr A 1203:146–152
25. Liebl W, Bayerl A, Stillner U, Schleifer KH (1989) High efficiency electroporation of intact Corynebacterium glutamicum cells. FEMS Microbiol Lett 65:299–304
26. Miyajima R, Shiio I (1970) Regulation of aspartate family amino acid biosynthesis in Brevibacterium flavum. III. Properties of homoserine dehydrogenase. J Biochem 68:311–319
27. Möckel B, Eggeling L, Sahm H (1994) Threonine dehydratases of Corynebacterium glutamicum with altered allosteric control: their generation and biochemical and structural analysis. Mol Microbiol 13:833–842
28. Morbach S, Sahm H, Eggeling L (1995) Use of feedback-resistant threonine dehydratases of Corynebacterium glutamicum to increase carbon flux towards l-isoleucine. Appl Environ Microbiol 61:4315–4320
29. Morbach S, Sahm H, Eggeling L (1996) l-Isoleucine Production with Corynebacterium glutamicum: further flux increase and limitation of export. Appl Environ Microbiol 62:4345–4351
30. Park JH, Jang YS, Lee JW, Lee SY (2011) Escherichia coli W as a new platform strain for the enhanced production of l-valine by systems metabolic engineering. Biotechnol Bioeng 108:1140–1147
31. Park JH, Oh JE, Lee KH, Kim JY, Lee SY (2012) Rational design of Escherichia coli for l-isoleucine production. ACS Synth Biol 1:532–540
32. Park SD, Lee JY, Sim SY, Kim Y, Lee HS (2007) Characteristics of methionine production by an engineered Corynebacterium glutamicum strain. Metab Eng 9:327–336
33. Park JH, Lee KH, Kim TY, Lee SY (2007) Metabolic engineering of Escherichia coli for the production of l-valine based on transcriptome analysis and in silico knockout simulation. Proc Natl Acad Sci USA 104:7797–7802
34. Park JH, Lee SY (2010) Fermentative production of branched chain amino acids: a focus on metabolic engineering. Appl Microbiol Biotechnol 85:491–506
35. Reinscheid DJ, Eikmanns BJ, Sahm H (1991) Analysis of a Corynebacterium glutamicum hom gene coding for a feedback-resistant homoserine dehydrogenase. J Bacteriol 173:3228–3230
36. Reinscheid DJ, Kronemeyer W, Eggeling L, Eikmanns BJ, Sahm H (1994) Stable expression of hom-1–thrB in Corynebacterium glutamicum and its effects on the carbon flux to threonine and related amino acids. Appl Environ Microbiol 60:126–132
37. Sahm H, Eggeling L, Morbach S (1999) Construction of l-isoleucine overproducing strains of Corynebacterium glutamicum. Naturwissenschaften 86:33–38
38. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
39. Shiio I, Miyajima R (1969) Concerted inhibition and its reversal by end products of aspartate kinase in Brevibacterium flavum. J Biochem 64:849–859
40. Simic P, Willuhn J, Sahm H, Eggeling L (2002) Identification of glyA (encoding serine hydroxymethyl transferase) and its use together with the exporter ThrE to increase l-threonine accumulation by Corynebacterium glutamicum. Appl Environ Microbiol 68:3321–3327
41. Vogt M, Krumbach K, Bang WG, van Ooyen J, Noack S, Klein B, Bott M, Eggeling L (2015) The contest for precursors: channelling l-isoleucine synthesis in Corynebacterium glutamicum without byproduct formation. Appl Microbiol Biotechnol 99:791–800
42. Vogt M, Haas S, Klaffl S, Polen T, Eggeling L, van Ooyen J, Bott M (2014) Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for l-leucine overproduction. Metab Eng 22:40–52
43. Xu D, Tan Y, Huan X, Hu X, Wang X (2010) Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J Microbiol Methods 80:86–92
44. Wang J, Wen B, Wang J, Xu Q, Zhang C, Chen N, Xie X (2013) Enhancing l-isoleucine production by thrABC overexpression combined with alaT deletion in Corynebacterium glutamicum. Appl Biochem Biotechnol 171:20–30
45. Yin L, Hu X, Xu D, Ning J, Chen J, Wang X (2012) Co-expression of feedback-resistant threonine dehydratase and acetohydroxy acid synthase increase l-isoleucine production in Corynebacterium glutamicum. Metab Eng 14:542–550
46. Yin L, Zhao J, Chen C, Hu X, Wang X (2014) Increasing l-isoleucine production in Corynebacterium glutamicum by enhancing the carbon flux in its biosynthesis pathway and NADPH supply. Biotechnol Bioprocess E 19:132–142
47. Yoshida A, Tomita T, Kurihara T, Fushinobu S, Kuzuyama T, Nishiyama M (2007) Structural insight into concerted inhibition of α2β2-type aspartate kinase from Corynebacterium glutamicum. J Mol Biol 368:521–536
48. Yoshida A, Tomita T, Kuzuyama T, Nishiyama M (2010) Mechanism of concerted inhibition of α2β2-type hetero-oligomeric aspartate kinase from Corynebacterium glutamicum. J Biol Chem 285:27477–27486
49. Yu X, Jin H, Liu W, Wang Q, Qi Q (2015) Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose. Microb Cell Fact 14:183–192
50. Zhao J, Hu X, Li Y, Wang X (2015) Overexpression of ribosome elongation factor G and recycling factor increases l-isoleucine production in Corybacterium glutamicum. Appl Microbiol Biotechnol 99:4795–4805