Identification of glyA (encoding serine hydroxymethyltransferase) and its use together with the exporter THrE to increase L-threonine accumulation by Corynebacterium glutamicum

Applied and Environmental Microbiology

Volumn 68, Issue 7, 2002, Pages 3321-3327

  Simic, Petra ^a   Willuhn, Juliane ^a   Sahm, Hermann ^a   Eggeling, Lothar ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CLONING; DEGRADATION; ENZYMES; GENES;

INDENTIFICATION;

MICROBIOLOGY;

GLYCINE HYDROXYMETHYLTRANSFERASE; THREONINE;

BACTERIUM;

ALLELE; ARTICLE; BACTERIUM IDENTIFICATION; BIOACCUMULATION; CATALYSIS; CORYNEBACTERIUM GLUTAMICUM; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; FEEDBACK SYSTEM; GENE MUTATION; GENETIC CODE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE;

AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; BACTERIAL PROTEINS; CARRIER PROTEINS; CLONING, MOLECULAR; CORYNEBACTERIUM; GLYCINE; GLYCINE HYDROXYMETHYLTRANSFERASE; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; THREONINE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; UNCULTURED ACTINOMYCETE;

EID: 0036304303     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.7.3321-3327.2002     Document Type: Article

References (46)

1. Serine hydroxymethyltransferase: Origin of substrate specificity
2. + oxidoreductase
3. Bacterial catabolism of L-threonine. Threonine degradation initiated by L-threonine hydrolyase (deaminating) in a species of Corynebacterium
4. Regulation and specificity of the LysE-mediated export of amino acids by Corynebacterium glutamicum
5. Effect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799
6. Metabolic engineering for L-lysine production by Corynebacterium glutamicum
7. Amino acid production: Principles of metabolic engineering
8. L-Glutamate and L-lysine: Traditional products with impetuous developments
9. The cell wall barrier of Corynebacterium glutamicum and amino acid efflux
10. L-Glutamate efflux with Corynebacterium glutamicum: Why is penicillin treatment or Tween addition doing the same?
11. The fruits of molecular physiology: Engineering the L-isoleucine biosynthesis pathway in Corynebacterium glutamicum
12. Amino acids
13. Amplification of three biosynthesis genes in Corynebacterium glutamicum and its influence on carbon flux in different strains
14. Organization and regulation of the Corynebacterium glutamicum hom-thrB and thrC loci
15. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants
16. The molecular structure of the Corynebacterium glutamicum threonine synthase gene
17. Bulk amino-acid fermentation: Technology and commodity trading
18. Hyperproduction of tryptophan by Corynebacterium glutamicum with the modified pentose phosphate pathway
19. Isoleucine synthesis in Corynebacterium glutamicum: Molecular analysis of the ilvB-ilvN-ilvC operon
20. Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum
21. High performance liquid chromatographic detection of subpicomole amounts of amino acids by pecolumn fluorescence derivatization with o-phthdialdehyde
22. Gene cloning, biochemical characterization and physiological characterization of a thermostable low-specificity L-threonine aldolase from Escherichia coli
23. Gene cloning, nucleotide sequencing, and purification of the low-specificity L-threonine aldolase from Pseudomonas sp. strain NCIMB 10558
24. Characterization of the MetR binding sites for the glyA gene of Escherichia coli
25. Escherichia coli cis- and trans-acting mutations that increase glyA gene expression
26. pH-dependent decarboxylation of 2-amino-3-ketobutyrate, the unstable intermediate in the threonine dehydrogenase-initiated pathway for threonine utilization
27. Affinity purification and characterization of serine hydroxy-methyltransferases from rat liver
28. Influence of increased aspartate availability on lysine formation by a recombinant strain of Corynebacterium glutamicum and utilization of fumarate
29. Threonine production by coexistence of cloned genes encoding homoserine dehydrogenase and homoserine kinase in Brevibacterium lactofermentum
30. Expression of genes of lipid synthesis and altered lipid composition modulates L-glutamate efflux of Corynebacterium glutamicum
31. Serine hydroxymethyltransferase and threonine aldolase: Are they identical?
32. Threonine diffusion and threonine transport in Corynebacterium glutamicum and their role in threonine production
33. Promoters from Corynebacterium glutamicum: Cloning, molecular analysis and search for a consensus motif
34. Analysis of a Coryne-bacterium glutamicum hom gene coding for a feedback-resistant homoserine dehydrogenase
35. Stable expression of hom1-thrB in Corynebacterium glutamicum and its effect on the carbon flux to threonine and related amino acids
36. Crystal structure at 2.8 Å resolution of E. coli serine hydroxymethyltransferase in complex with glycine substrate and 5-formyl tetrahydrofolate
37. Small mobilizable multipurpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: Selection of defined deletions in the chromosome of Corynebacterium glutamicum
38. Serine transhydroxymethylase: Identification as the threonine and allothreonine aldolases
39. L-threonine export: Use of peptides to identify a new translocator from Corynebacterium glutamicum
40. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria
41. TetZ, a new tetracycline resistance determinant discovered in gram-positive bacteria, shows high homology to gram-negative regulated efflux systems
42. A heat shock following electroporation induces highly efficient transformation of Coryne-bacterium glutamicum with xenogeneic plasmid DNA
43. Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum
44. A new type of transporter with a new type of cellular function: L-lysine export from Corynebacterium glutamicum
45. The ubiquitous ThrE family of putative transmembrane amino acid efflux transporters