Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production

Applied and Environmental Microbiology

Volumn 65, Issue 7, 1999, Pages 3100-3107

  Guillouet, S ^a   Rodal, A A ^b   An, G H ^c   Lessard, P A ^a   Sinskey, A J ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c SunMoon University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

FOOD ADDITIVE; ISOLEUCINE; THREONINE DEHYDRATASE;

AMINO ACID SYNTHESIS; ARTICLE; BIODEGRADATION; CORYNEBACTERIUM GLUTAMICUM; ENZYME REGULATION; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION;

CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

EID: 0033014686     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.7.3100-3107.1999     Document Type: Article

References (21)

1. Benner, S. A. 1989. Enzyme kinetics and molecular evolution. Chem. Rev. 89:789-806.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Colón, G. E., T. T. Nguyen, M. S. Jetten, A. J. Sinskey, and G. Stephanopoulos. 1995. Production of isoleucine by overexpression of ilvA in a Corynebacterium lactofermentum threonine producer. Appl. Microbiol. Biotechnol. 43:482-488.
4. Duncan, B. K., and B. Weiss. 1982. Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli. J. Bacteriol. 151:750-755.
5. Eggeling, I., C. Cordes, L. Eggeling, and H. Sahm. 1987. Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of α-ketobutyrate to L-isoleucine. Appl. Microbiol. Biotechnol. 25: 346-351.
6. Feldman, D. A., and P. Datta. 1975. Catabolite inactivation of bidegradative threonine dehydratase of Escherichia coli. Biochemistry 14:1760-1767.
7. Follette, M. T., H. K. Shin, and A. J. Sinskey. 1988. Organization and regulation of the C. glutamicum hom-thrB and thrC loci. Mol. Microbiol. 2:53-62.
8. Goss, T. J., and P. Datta. 1985. Molecular cloning and expression of the biodegradative threonine dehydratase gene (tdc) of Escherichia coli K12. Mol. Gen. Genet. 201:308-314.
9. Hashiguchi, K., H. Kojima, K. Sato, and K. Sano. 1997. Effects of an Escherichia coli ilvA mutant gene encoding feedback-resistant threonine deaminase on L-isoleucine production by Brevibacterium flavum. Biosci. Biotechnol. Biochem. 61:105-108.
10. Jetten, M. S. M., M. T. Follettie, and A. J. Sinskey. 1995. Effect of different levels of aspartokinase on the lysine production by Corynebacterium lactofermentum. Appl. Microbiol. Biotechnol. 43:76-82.
11. Jetten, M. S. M., M. E. Gubler, M. M. McCormick, G. E. Colón, M. T. Follettie, and A. J. Sinskey. 1993. Molecular organization and regulation of the biosynthetic pathway for aspartate-derived amino acids in Corynebacterium glutamicum, p. 97-104. In R. H. Baltz, G. Hegeman, and P. L. Skatrud (ed.), Industrial microorganisms: basic and applied molecular genetics. American Society for Microbiology, Washington, D.C.
12. Jetten, M. S. M., G. A. Pitoc, M. T. Follettie, and A. J. Sinskey. 1994. Regulation of phospho(enol)-pyruvate-and oxaloacetate-converting enzymes in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 41:47-52.
13. Kelle, R., T. Hermann, D. Weuster-Botw, L. Eggeling, R. Kramer, and C. Wandrey. 1996. Glucose-controlled L-isoleucine fed-batch production with recombinant strains of Corynebacterium glutamicum. J. Biotechnol. 50:123-136.
14. Lessard, P. A., S. Guillouet, L. B. Willis, and A. J. Sinskey. 1999. Coryne-bacteria, brevibacteria, p. 729-740. In M. C. Flickinger and S. W. Drew (ed.), The encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation, vol. 2. John Wiley & Sons, New York, N. Y.
15. Mockel, B., L. Eggeling, and H. Sahm. 1994. Threonine dehydratases of Corynebacterium glutamicum with altered allosteric control: their generation and biochemical and structural analysis. Mol. Microbiol. 13:833-842.
16. Morbach, S., H. Sahm, and L. Eggeling. 1995. Use of feedback-resistant threonine dehydratases of Corynebacterium glutamicum to increase carbon flux towards L-isoleucine. Appl. Environ. Microbiol. 61:4315-4320.
17. Pittard, J., and E. A. Adelberg. 1963. Gene transfer by F' strains of E. coli K-12. II. Interaction between F-merogenote and chromosome during transfer. J. Bacteriol. 85:1402-1408.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Tsuchida, T., and H. Momose. 1975. Genetic changes of regulatory mechanisms occurred in leucine and valine producing mutants derived from Brevibacterium lactofermentum 2256. Agric. Biol. Chem. 39:2193-2198.
20. Umbarger, H. E., and B. Brown. 1957. Threonine deamination in Escherichia coli. II. Evidence for the two L-threonine deaminases. J. Bacteriol. 73:105-112.
21. Zhang, Y., J. Praszkier, A. Hodgson, and A. J. Pittard. 1994. Molecular analysis and characterization of a broad-host-range plasmid, pEP2. J. Bacteriol. 176:5718-5728.