Enhancing the carbon flux and NADPH supply to increase L-isoleucine production in Corynebacterium glutamicum

Biotechnology and Bioprocess Engineering

Volumn 19, Issue 1, 2014, Pages 132-142

  Yin, Lianghong ^a   Zhao, Jianxun ^a   Chen, Cheng ^a   Hu, Xiaoqing ^a   Wang, Xiaoyuan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

brnFE; Corynebacterium glutamicum; ilvA; ilvBN; L isoleucine; lrp; NADPH; ppnk

Indexed keywords

ENCODING (SYMBOLS); PLANTS (BOTANY); STRAIN;

BRNFE; CORYNEBACTERIUM GLUTAMICUM; ILVA; ILVBN; L-ISOLEUCINE; LRP; NADPH; PPNK;

AMINO ACIDS;

ASPARTIC ACID; CARBON; GLUCOSE; ISOLEUCINE; LYSINE; METHIONINE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; THREONINE;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL STRAIN; BIOMASS; BIOTECHNOLOGICAL PRODUCTION; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; ENZYME SPECIFICITY; FED BATCH FERMENTATION; NONHUMAN; PRODUCTIVITY; REAL TIME POLYMERASE CHAIN REACTION;

EID: 84896930648     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-013-0416-z     Document Type: Article

References (39)

1. Park, J. H. and S. Y. Lee (2010) Metabolic pathways and fermentative production of L-aspartate family amino acids. J. Biotechnol. 5: 560-577.
2. Eggeling, L., S. Morbach, and H. Sahm (1997) The fruits of molecular physiology: Engineering the L-isoleusine biosynthesis pathway in Corynebacterium glutamicum. J. Biotechnol. 56: 167-182. (Pubitemid 27400369)
3. Umbarger, H. E. (1987) Biosynthesis of the branched-chain amino acids. pp. 352-367. In: F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (eds.). Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington D. C., USA.
4. Morinaga, Y., H. Takagi, M. Ishida, K. Miwa, T. Sato, S. Nakamori, and K. Sano (1987) Threonine production by co-existence of cloned genes coding homoserine dehydrogenase and homoserine kinase in Brevibacterium lactofermentum. Agric. Biol. Chem. 51: 93-100.
5. Colon, G. E., M. S. M. Jetten, T. T. Nguyen, M. E. Gubler, M. T. Follettie, A. J. Sinskey, and G. Stephanopoulos (1994) Effect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799. Appl. Environ. Microbiol. 61: 74-78.
6. Dong, X., P. J. Quinn, and X. Wang (2011) Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the production of L-threonine. Biotechnol. Adv. 29: 11-23.
7. Morbach, S., H. Sahm, and L. Eggeling (1995) Use of feedback-resistant threonine dehydratases of Corynebacterium glutamicum to increase carbon flux towards L-isoleucine. Appl. Environ. Microbiol. 61: 4315-4320.
8. Elišáková, V., M. Pátek, J. Holátko, J. Nešvera, D. Leyval, J. L. Goergen, and S. Delaunay (2005) Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum. Appl. Environ. Microbiol. 71: 207-213. (Pubitemid 40116269)
9. Kennerknecht, N., H. Sahm, M. -R. Yen, M. Pátek, M. H. Saier, and L. Eggeling (2002) Export of L-isoleucine from Corynebacterium glutamicum: A two-gene-encoded member of a new translocator family. J. Bacteriol. 184: 3947-3956. (Pubitemid 34705453)
10. Yin, L., F. Shi, X. Hu, C. Chen, and X. Wang (2013) Increasing L-isoleucine production in Corynebacterium glutamicum by overexpressing global regulator Lrp and two-component export system BrnFE. J. Appl. Microbiol. 114: 1369-1377.
11. Ikeda, S., I. Fujita, and F. Yoshinaga (1976) Screening of L-isoleucine producers among ethionine resistant mutants of L-threonine producing bacteria. Agric. Biol. Chem. 40: 511-516.
12. Kase, H. and K. Nakayama (1977) L-Isoleucine production by analog-resistant mutants derived from threonine-producing strain of Corynebacterium glutamicum. Agric. Biol. Chem. 41: 109-116.
13. Kalinowski, J., B. Bathe, D. Bartels, N. Bischoff, M. Bott, A. Burkovski, N. Dusch, L. Eggeling, B. J. Eikmanns, and L. Gaigalat (2003) The complete Corynebacterium glutamicum ATCC13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins. J. Biotechnol. 104: 5-25. (Pubitemid 37011345)
14. Morbach, S., R. Kelle, S. Winkels, H. Sahm, and L. Eggeling (1996) Engineering the homoserine dehydrogenase and threonine dehydratase control points to analyse flux towards L-isoleucine in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 45: 612-620. (Pubitemid 26226614)
15. Eggeling, L., H. Sahm, and A. A. de Graaf (1996) Quantifying and directing metabolite flux: Application to amino acid overproduction. Metab. Eng. 54: 1-30.
16. Yin, L., X. Hu, D. Xu, J. Ning, J. Chen, and X. Wang (2012) Coexpression of feedback-resistant threonine dehydratase and acetohydeoxy acid synthase increase L-isoleucine production in Corynebacterium glutamicum. Metab. Eng. 14: 542-550.
17. Shi, F., X. J. Huan, X. Y. Wang, and J. F. Ning (2012) Overexpression of NAD kinases improves the L-isoleucine biosyntheis in Corynebacterium glutamicum ssp. lactofermentum. Enz. Microb. Tech. 51: 73-80.
18. Peng, Z. J., J. Fang, J. H. Li, L. Liu, G. C. Du, J. Chen, X. Y. Wang, J. F. Ning, and L. M. Cai (2010) Combined dissolved oxygen and pH control strategy to improve the fermentative production of L-isoleucine by Brevibacterium lactofermentum. Bioproc. Biosyst. Eng. 33: 339-345.
19. rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
20. Xu, D., Y. Tan, X. Huan, X. Hu, and X. Wang (2010) Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J. Microbiol. Meth. 80: 86-92.
21. Miller, L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426-428.
22. Koros, A., Z. S. Varga, and P. Molnar (2008) Simultaneous analysis of amino acids and amines as their ophthalaldehyde-ethanethiol-9-fluorenylmethyl chloroformate derivatives in cheese by high-performance liquid chromatography. J. Chromatogr. A. 1203: 146-152.
23. Livak, K. J. and T. D. Schmittgen (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25: 402-408. (Pubitemid 34164012)
24. Nolden, L., M. Farwick, R. Krämer, and Burkovski (2001) Glutamine synthetases of Corynebacterium glutamicum: Transcriptional control and regulation of activity. FEMS Microbiol. Lett. 201: 91-98. (Pubitemid 32607525)
25. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
26. Miyajima, R., S. -I. Otsuka, and I. Shiio (1968) Regulation aspartate family amino acid biosynthesis in Brevibacterium flavum. J. Biochem. 63: 139-148.
27. Guillouet, S., A. A. Rodal, and A. J. Sinskey (1999) Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production. Appl. Environ. Microbiol. 65: 3100-3107. (Pubitemid 29315586)
28. Westerfeld, W. W. (1945) A colorimetrie determination of blood acetoin. J. Biol. Chem. 161: 495-502.
29. Eggeling, I., L. Eggeling, and H. Sahm (1987) Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of α-ketobutyrate to L-isoleucine. Appl. Microbiol. Biotechnol. 25: 346-351. (Pubitemid 17013005)
30. Shi, F., S. Kawai, S. Mori, E. Kono, and K. Murata (2005) Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae. FEBS. J. 272: 3337-3379.
31. Lee, I. Y., M. K. Kim, Y. H. Park, and S. Y. Lee (1996) Regulatory effects of cellular nicotinamide nucleotides and enzyme activities on poly(3-hydroxybutyrate) synthesis in recombinant Escherichia coli. Biotechnol. Bioeng. 52: 707-712. (Pubitemid 26397155)
32. Zerez, C. R., S. J. Lee, and K. R. Tanaka (1987) Spectrophotometric determination of oxidized and reduced pyridine nucleotides in erythrocytes using a single extraction procedure. Anal. Biochem. 164: 367-373. (Pubitemid 17123608)
33. Kawa, I. S., S. Mori, T. Mukai, W. Hashimoto, and K. Murata (2001) Molecular characterization of Escherichia coli NAD kinase. Eur. J. Biochem. 268: 4359-4365. (Pubitemid 32862898)
34. Grose, J. H., L. Joss, S. F. Velick, and J. R. Roth (2006) Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress. Proc. Natl. Acad. Sci. USA 103: 7601-7606.
35. Sauer, U., F. Canonaco, S. Heri, A. Perrenoud, and E. Fischer (2004) The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J. Biol. Chem. 279: 6613-6619. (Pubitemid 38248798)
36. Koffas, M. and G. Stephanopoulos (2005) Strain improvement by metabolic engineering: lysine production as a case study for systems biology. Curr. Opin. Biotechnol. 16: 361-366. (Pubitemid 40806476)
37. Lee, K. H., J. H. Park, T. Y. Kim, H. U. Kim, and S. Y. Lee (2007) Systems metabolic engineering of Escherichia coli for L-threonine production. Mol. Syst. Biol. 3: 149.
38. Kind, S., J. Becher, and C. Wittmann (2012) Increased lysine production by flux coupling of the tricarboxylic acid cycle and the lysine biosynthetic pathway-Metabolic engineering of the availability of succinyl-CoA in Corynebacterium glutamicum. Metab. Eng. 15: 184-195.
39. Becker, J., O. Zelder, S. Häfner, H. Schröder, and C. Wittmann (2011) From zero to hero-Design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab. Eng. 13: 159-168.