Overexpression of ribosome elongation factor G and recycling factor increases l-isoleucine production in Corynebacterium glutamicum

Applied Microbiology and Biotechnology

Volumn 99, Issue 11, 2015, Pages 4795-4805

  Zhao, Jianxun ^a   Hu, Xiaoqing ^a   Li, Ye ^a   Wang, Xiaoyuan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Corynebacterium glutamicum; Elongation factor G; frr; fusA; l Isoleucine production; Ribosome recycling factor

Indexed keywords

BACTERIA; BIOSYNTHESIS; ELONGATION; FERMENTATION; GENES; MACROMOLECULES; POLYMERASE CHAIN REACTION; PROTEINS; RECYCLING; RNA; SYNTHESIS (CHEMICAL); TRANSCRIPTION;

CORYNEBACTERIUM GLUTAMICUM; ELONGATION FACTOR; FRR; FUSA; L-ISOLEUCINE; RIBOSOME RECYCLING FACTOR;

AMINO ACIDS;

ELONGATION FACTOR G; ISOLEUCINE; RIBOSOME PROTEIN; RIBOSOME RECYCLING FACTOR;

BIOSYNTHESIS; CORYNEBACTERIUM GLUTAMICUM; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

CORYNEBACTERIUM GLUTAMICUM; GENE EXPRESSION; GENE EXPRESSION PROFILING; ISOLEUCINE; METABOLIC ENGINEERING; PEPTIDE ELONGATION FACTOR G; RIBOSOMAL PROTEINS;

EID: 84939956655     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6458-8     Document Type: Article

References (42)

1. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1):248–254
2. Cremer J, Eggeling L, Sahm H (1991) Control of the lysine biosynthesis sequence in Corynebacterium glutamicum as analyzed by overexpression of the individual corresponding genes. Appl Environ Microbiol 57(6):1746–1752
3. Defeu S, Reimold C, Linne U, Knust T, Gescher J, Graumann PL (2010) Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Proc Natl Acad Sci U S A 107(7):3163–3168
4. Eggeling I, Cordes C, Eggeling L, Sahm H (1987) Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of α-ketobutyrate to L-isoleucine. Appl Microbiol Biotechnol 25(4):346–351
5. Eggeling L, Morbach S, Sahm H (1997) The fruits of molecular physiology: engineering the L-isoleucine biosynthesis pathway in Corynebacterium glutamicum. J Biotechnol 56(3):167–182
6. Elisáková V, Pátek M, Holátko J, Nesvera J, Leyval D, Goergen JL, Delaunay S (2005) Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum. Appl Environ Microbiol 71(1):207–213
7. Guillouet S, Rodal AA, An GH, Lessard PA, Sinskey AJ (1999) Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production. Appl Environ Microbiol 65(7):3100–3107
8. Holtkamp W, Cunha C, Peske F, Konevega L, Wintermeyer W, Rodnina M (2014) GTP hydrolysis by EF-G synchronizes tRNA movement on small and large ribosomal subunits. EMBO J 33(9):1073–1085
9. Hosaka T, Xu J, Ochi K (2006) Increased expression of ribosome recycling factor is responsible for the enhanced protein synthesis during the late growth phase in an antibiotic-overproducing Streptomyces coelicolor ribosomal rpsL mutant. Mol Microbiol 61(4):883–897
10. Hou X, Chen X, Zhang Y, Qian H, Zhang W (2012) (L)-Valine production with minimization of by-products’ synthesis in Corynebacterium glutamicum and Brevibacterium flavum. Amino Acids 43(6):2301–2311
11. Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A (2003) The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins. J Biotechnol 104(1–3):5–25
12. Katunin VI, Savelsbergh A, Rodnina MV, Wintermeyer W (2002) Coupling of GTP hydrolysis by elongation factor G to translocation and factor recycling on the ribosome. Biochemistry 41(42):12806–12812
13. Kavaliauskas D, Nissen P, Knudsen CR (2012) The busiest of all ribosomal assistants: elongation factor Tu. Biochemistry 51(13):2642–2651
14. Korös A, Varga Z, Molnár-Perl I (2008) Simultaneous analysis of amino acids and amines as their o-phthalaldehyde-ethanethiol-9-fluorenylmethyl chloroformate derivatives in cheese by high-performance liquid chromatography. J Chromatogr A 1203(2):146–152
15. Lancaster L, Kiel MC, Kaji A, Noller HF (2002) Orientation of ribosome recycling factor in the ribosome from directed hydroxyl radical probing. Cell 111(1):129–140
16. Li L, Guo J, Wen Y, Chen Z, Song Y, Li J (2010) Overexpression of ribosome recycling factor causes increased production of avermectin in Streptomyces avermitilis strains. J Ind Microbiol Biotechnol 37(7):673–679
17. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) Method. Methods 25(4):402–408
18. Ma Z, Tao L, Bechthold A, Shentu X, Bian Y, Yu X (2014) Overexpression of ribosome recycling factor is responsible for improvement of nucleotide antibiotic-toyocamycin in Streptomyces diastatochromogenes 1628. Appl Microbiol Biotechnol 98(11):5051–5058
19. Maracci C, Peske F, Dannies E, Pohl C, Rodnina MV (2014) Ribosome-induced tuning of GTP hydrolysis by a translational GTPase. Proc Natl Acad Sci U S A 111(40):14418–14423
20. Miyajima R, Otsuka SI, Shiio I (1968) Regulation aspartate family amino acid biosynthesis in Brevibacterium flavum. J Biochem 63(2):139–148
21. Morbach S, Sahm H, Eggeling L (1995) Use of feedback-resistant threonine dehydratases of corynebacterium glutamicum to increase carbon flux towards l-isoleucine. Appl Environ Microbiol 61(12):4315–4320
22. Park JH, Lee SY (2010a) Metabolic pathways and fermentative production of L-aspartate family amino acids. Biotechnol J 5(6):560–577
23. Park JH, Lee SY (2010b) Fermentative production of branched chain amino acids: a focus on metabolic engineering. Appl Microbiol Biotechnol 85(3):491–506
24. Park JH, Oh JE, Lee KH, Kim JY, Lee SY (2012) Rational design of Escherichia coli for L-isoleucine production. ACS Synth Biol 1(11):532–540
25. Rolland N, Janosi L, Block MA, Shuda M, Teyssier E, Miège C, Chéniclet C, Carde JP, Kaji A, Joyard J (1999) Plant ribosome recycling factor homologue is a chloroplastic protein and is bactericidal in Escherichia coli carrying temperature-sensitive ribosome recycling factor. Proc Natl Acad Sci U S A 96:5464–5469
26. Savelsbergh A, Rodnina V, Wintermeyer W (2009) Distinct functions of elongation factor G in ribosome recycling and translocation. RNA 15(5):772–780
27. Schmeing TM, Ramakrishnan V (2009) What recent ribosome structures have revealed about the mechanism of translation. Nature 461(7268):1234–1242
28. Shi F, Li K, Huan X, Wang X (2013) Expression of NAD(H) kinase and glucose-6-phosphate dehydrogenase improve NADPH supply and L-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum. Appl Biochem Biotechnol 171(2):504–521
29. Shoji S, Walker SE, Fredrick K (2009) Ribosomal translocation: one step closer to the molecular mechanism. ACS Chem Biol 4(2):93–107
30. Steitz TA (2008) A structural understanding of the dynamic ribosome machine. Nat Rev Mol Cell Biol 9(3):242–253
31. Teyssier E, Hirokawa G, Tretiakova A, Jameson B, Kaji A, Kaji H (2003) Temperature sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF). Nucleic Acids Res 31:4218–4226
32. Vogt M, Krumbach K, Bang WG, van Ooyen J, Noack S, Klein B, Bott M, Eggeling L (2015) The contest for precursors: channelling L-isoleucine synthesis in Corynebacterium glutamicum without byproduct formation. Appl Microbiol Biotechnol 99(2):791–800
33. Vrljic M, Kronemeyer W, Sahm H, Eggeling L (1995) Unbalance of L-lysine flux in Corynebacterium glutamicum and its use for the isolation of excretion-defective mutants. J Bacteriol 177(14):4021–4027
34. Westerfeld WW (1945) A colorimetrie determination of blood acetoin. J Biol Chem 161:495–502
35. Xie X, Xu L, Shi J, Xu Q, Chen N (2012) Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW. J Ind Microbiol Biotechnol 39(10):1549–1556
36. Xu D, Tan Y, Huan X, Hu X, Wang X (2010) Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J Microbiol Methods 80(1):86–92
37. Xu L, Xie X, Shi J, Xu Q, Chen N (2013) Expression of the Escherichia coli TdcB gene encoding threonine dehydratase in L-isoleucine-overproducing Corynebacterium Glutamicum Yilw. Appl Biochem Microbiol 49(2):125–129
38. Yin L, Hu X, Xu D, Ning J, Chen J, Wang X (2012) Co-expression of feedback-resistant threonine dehydratase and acetohydroxy acid synthase increase L-isoleucine production in Corynebacterium glutamicum. Metab Eng 14(5):542–550
39. Yin L, Shi F, Hu X, Chen C, Wang X (2013) Increasing l-isoleucine production in Corynebacterium glutamicum by overexpressing global regulator Lrp and two-component export system BrnFE. J Appl Microbiol 114(5):1369–1377
40. Yin L, Hu X, Wang X (2014a) Proteomic analysis of L-isoleucine production by Corynebacterium glutamicum. J Pure Appl Microbiol 8(2):899–908
41. Yin L, Zhao J, Chen C, Hu X, Wang X (2014b) Enhancing the carbon flux and NADPH supply to increase L-isoleucine production in Corynebacterium glutamicum. Biotechnol Bioprocess Eng 19(1):132–142
42. Zhou J, Lancaster L, Donohue J, Noller H (2014) How the ribosome hands the A-site tRNA to the P site during EF-G-catalyzed translocation. Science 345(6201):1188–1191