Directed evolution of human scFvs in DT40 cells

Protein Engineering, Design and Selection

Volumn 29, Issue 2, 2015, Pages 39-48

  Lim, Alfred W Y ^a   Williams, Gareth T ^a   Rada, Cristina ^a   Sale, Julian E ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

affinity maturation; in vitro selection; monoclonal antibodies; scFv; somatic hypermutation

Indexed keywords

MONOCLONAL ANTIBODIES;

AFFINITY MATURATION; DIRECTED EVOLUTION; IMMUNOGLOBULIN GENES; IN-VITRO; NANOMOLAR AFFINITY; SCFV; SINGLE CHAIN VARIABLE FRAGMENTS; SOMATIC HYPERMUTATION;

ANTIBODIES;

IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN LAMBDA CHAIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; ANTIGEN; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; PEPTIDE LIBRARY;

ANIMAL CELL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; ARTICLE; CELL EXPANSION; CELL LINE; CELL SELECTION; CHICKEN; CONTROLLED STUDY; CONVERGENT EVOLUTION; GENE EXPRESSION; GENE LIBRARY; GENE LOCUS; GENE OVEREXPRESSION; GENE REARRANGEMENT; HUMAN CELL CULTURE; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SOMATIC HYPERMUTATION; ANIMAL; DIRECTED MOLECULAR EVOLUTION; GENETICS; HEK293 CELL LINE; HUMAN; IMMUNOGLOBULIN GENE; IMMUNOLOGY; MOUSE; MUTATION; PEPTIDE LIBRARY; PROCEDURES;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIGENS; CELL LINE; CHICKENS; DIRECTED MOLECULAR EVOLUTION; GENES, IMMUNOGLOBULIN; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN G; MICE; MUTATION; PEPTIDE LIBRARY; SINGLE-CHAIN ANTIBODIES;

EID: 84961665614     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzv058     Document Type: Article

References (35)

1. Arakawa, H., Saribasak, H. and Buerstedde, J.-M. (2004) Plos. Biol., 2, E179.
2. Arakawa, H., Kudo, H., Batrak, V., Caldwell, R.B., Rieger, M.A., Ellwart, J.W. and Buerstedde, J.-M. (2008) Nucl. Acids. Res., 36, e1.
3. Betz, A.G., Rada, C., Pannell, R., Milstein, C. and Neuberger, M.S. (1993) Proc. Natl Acad. Sci. USA, 90, 2385-2388.
4. Boder, E.T., Midelfort, K.S. andWittrup, K.D. (2000) Proc. Natl Acad. Sci. USA, 97, 10701-10705.
5. Boulter-Bitzer, J.I., Lee, H. and Trevors, J.T. (2009) J. Parasitol., 95, 75-81.
6. Breitling, F., Dübel, S., Seehaus, T., Klewinghaus, I. and Little, M. (1991) Gene, 104, 147-153.
7. Brindle, N.P.J., Sale, J.E., Arakawa, H., Buerstedde, J.-M., Nuamchit, T., Sharma, S. and Steele, K.H. (2013) J. Biol. Chem., 288, 33205-33212.
8. Brüggemann, M., Caskey, H.M., Teale, C., Waldmann, H., Williams, G.T., Surani, M.A. and Neuberger, M.S. (1989) Proc. Natl Acad. Sci. USA, 86, 6709-6713.
9. Buerstedde, J.M., Reynaud, C.A., Humphries, E.H., Olson, W., Ewert, D.L. and Weill, J.C. (1990) EMBO J., 9, 921-927.
10. Chowdhury, P.S. and Pastan, I. (1999) Nat. Biotechnol., 17, 568-572.
11. Cumbers, S.J., Williams, G.T., Davies, S.L., Grenfell, R.L., Takeda, S., Batista, F. D., Sale, J.E. and Neuberger, M.S. (2002) Nat. Biotechnol., 20, 1129-1134.
12. De Wildt, R.M., Mundy, C.R., Gorick, B.D. and Tomlinson, I.M. (2000) Nat. Biotechnol., 18, 989-994.
13. Ecker, D.M., Jones, S.D. and Levine, H.L. (2015) MAbs, 7, 9-14.
14. Gram, H., Marconi, L.A., Barbas, C.F., Collet, T.A., Lerner, R.A. and Kang, A.S. (1992) Proc. Natl Acad. Sci. USA, 89, 3576-3580.
15. Green, L.L., Hardy, M.C. and Maynard-Currie, C.E., et al. (1994) Nat. Genet., 7, 13-21.
16. James, L.C., Roversi, P. and Tawfik, D.S. (2003) Science, 299, 1362-1367.
17. Kang, A.S., Barbas, C.F., Janda, K.D., Benkovic, S.J. and Lerner, R.A. (1991) Proc. Natl Acad. Sci. USA, 88, 4363-4366.
18. Kim, S., Humphries, E.H., Tjoelker, L., Carlson, L. and Thompson, C.B. (1990) Mol. Cell. Biol., 10, 3224-3231.
19. Lebecque, S.G. and Gearhart, P.J. (1990) J. Exp. Med., 172, 1717-1727.
20. Lonberg, N., Taylor, L.D., Harding, F.A., et al. (1994) Nature, 368, 856-859.
21. McCafferty, J., Griffiths, A.D., Winter, G. and Chiswell, D.J. (1990) Nature, 348, 552-554.
22. Ponsel, D., Neugebauer, J., Ladetzki-Baehs, K. and Tissot, K. (2011) Molecules, 16, 3675-3700.
23. Pruzina, S., Williams, G.T., Kaneva, G., et al. (2011) Protein Eng. Des. Sel., 24, 791-799.
24. Rada, C. and Milstein, C. (2001) EMBO J., 20, 4570-4576.
25. Rada, C., González-Fernández, A., Jarvis, J.M. and Milstein, C. (1994) Eur. J. Immunol., 24, 1453-1457.
26. Robert, R., Jacobin-Valat, M.-J., Daret, D., Miraux, S., Nurden, A.T., Franconi, J.-M. and Clofent-Sanchez, G. (2006) J. Biol. Chem., 281, 40135-40143.
27. Sale, J.E. and Neuberger, M.S. (1998) Immunity, 9, 859-869.
28. Sale, J.E., Calandrini, D.M., Takata, M., Takeda, S. and Neuberger, M.S. (2001) Nature, 412, 921-926.
29. Seo, H., Masuoka, M., Murofushi, H., Takeda, S., Shibata, T. and Ohta, K. (2005) Nat. Biotechnol., 23, 731-735.
30. Shu, L., Qi, C.F., Schlom, J. and Kashmiri, S.V. (1993) Proc. Natl Acad. Sci. USA, 90, 7995-7999.
31. Steidl, S., Ratsch, O., Brocks, B., Dürr, M. and Thomassen-Wolf, E. (2008) Mol. Immunol., 46, 135-144.
32. Tiller, T., Meffre, E., Yurasov, S., Tsuiji, M., Nussenzweig, M.C. and Wardemann, H. (2008) J. Immunol. Methods., 329, 112-124.
33. Vaughan, T.J., Williams, A.J., Pritchard, K., et al. (1996) Nat. Biotechnol., 14, 309-314.
34. Wang, M., Yang, Z., Rada, C. and Neuberger, M.S. (2009) Nat. Struct. Mol. Biol., 16, 769-776.
35. Yabuki, M., Cummings, W.J., Leppard, J.B., Immormino, R.M., Wood, C.L., Allison, D.S., Gray, P.W., Tjoelker, L.W. and Maizels, N. (2012) PLoS One, 7, e36032.