Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display

Journal of Biological Chemistry

Volumn 288, Issue 46, 2013, Pages 33205-33212

  Brindle, Nicholas P J ^a   Sale, Julian E ^b   Arakawa, Hiroshi ^c   Buerstedde, Jean Marie ^d   Nuamchit, Teonchit ^a   Sharma, Shikha ^a   Steele, Kathryn H ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^c FIRC INSTITUTE OF MOLECULAR ONCOLOGY   (Italy)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE DISPLAYS; DIRECTED EVOLUTION; PROOF OF CONCEPT; RECEPTOR TYROSINE KINASE; SOMATIC HYPERMUTATION; SURFACE DISPLAYS; THERAPEUTIC TARGETS; VESSEL FORMATION;

AMINO ACIDS; CELL MEMBRANES; ENDOTHELIAL CELLS; LIGANDS; MACHINERY; MAINTENANCE;

BLOOD VESSELS;

ANGIOPOIETIN 1; ANGIOPOIETIN 2; ANGIOPOIETIN RECEPTOR;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL SURFACE DISPLAY; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENDOTHELIUM CELL; LIGAND BINDING; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; RECEPTOR BINDING; SOMATIC HYPERMUTATION;

ANGIOGENESIS; CELL SURFACE RECEPTOR; DIRECTED EVOLUTION; ENDOTHELIAL CELL; LIGAND-BINDING PROTEIN; PROTEIN ENGINEERING;

ANGIOPOIETIN-2; DIRECTED MOLECULAR EVOLUTION; HEK293 CELLS; HUMANS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTOR, TIE-2;

EID: 84887831643     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.510578     Document Type: Article

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