Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines

Nature Biotechnology

Volumn 20, Issue 11, 2002, Pages 1129-1134

  Cumbers, Sarah J ^a   Williams, Gareth T ^a   Davies, Sarah L ^a   Grenfell, Richard L ^a   Takeda, Shunichi ^b   Batista, Facundo D ^a   Sale, Julian E ^a   Neuberger, Michael S ^a  

^a Medical Research Laboratory of Biology   (United Kingdom)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ANTIGENS; CELLS; GENES;

STRATEGIC FRAMEWORK MUTATION;

BIOTECHNOLOGY;

B LYMPHOCYTE ANTIGEN; IMMUNOGLOBULIN; LIGAND; MONOCLONAL ANTIBODY; NANOPARTICLE; PROTEIN;

ANIMAL CELL; ANTIBODY AFFINITY; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; B CELL LYMPHOMA; B LYMPHOCYTE; CELL PROLIFERATION; CELL SELECTION; CHICKEN; COMPLEMENTARITY DETERMINING REGION; GENE MUTATION; GENETIC ANALYSIS; HUMAN; HUMAN CELL; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN VARIABLE REGION; LIGAND BINDING; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; RAT;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY FORMATION; BINDING SITES, ANTIBODY; BURKITT LYMPHOMA; CHICKENS; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IMMUNOGLOBULINS, INTRAVENOUS; MUTAGENESIS; RATS; REFERENCE VALUES; SELECTION (GENETICS); SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; STAPHYLOCOCCAL PROTEIN A; STREPTAVIDIN; TUMOR CELLS, CULTURED;

ANIMALIA; GALLUS GALLUS;

EID: 0036844226     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt752     Document Type: Article

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