Proteome-based bacterial identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS): A revolutionary shift in clinical diagnostic microbiology

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1854, Issue 6, 2015, Pages 528-537

  Nomura, Fumio ^a  

^a CHIBA UNIVERSITY HOSPITAL   (Japan)

Author keywords

Keywords Proteome MALDI TOF MS Clinical microbiology

Indexed keywords

BETA LACTAM; PROTEOME;

ANTIBIOTIC RESISTANCE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIAL MENINGITIS; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BLOOD ANALYSIS; CEREBROSPINAL FLUID; CLOSTRIDIUM PERFRINGENS; COST EFFECTIVENESS ANALYSIS; ENTEROCOCCUS FAECALIS; ENZYME ACTIVITY; ESCHERICHIA COLI; HOSPITAL INFECTION; HUMAN; INFECTION CONTROL; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PATIENT CARE; PHENOTYPE; PREVOTELLA BUCCAE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PYROLYSIS; REVIEW; STAPHYLOCOCCUS AUREUS; TANDEM MASS SPECTROMETRY; URINALYSIS; VANCOMYCIN RESISTANT ENTEROCOCCUS; BACTERIAL INFECTIONS; BACTERIUM; CLASSIFICATION; MASS SPECTROMETRY; METABOLISM; MICROBIOLOGY; PROCEDURES; PROTEOMICS;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE;

BACTERIA; BACTERIAL INFECTIONS; BACTERIAL TYPING TECHNIQUES; HUMANS; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84961288451     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2014.10.022     Document Type: Review

References (88)

1. A. Sun, C.L. Lam, and D.A. Wong Expanded newborn screening for inborn errors of metabolism Adv. Pediatr. 59 2012 209 245
2. A.H. Wu, R. Gerona, P. Armenian, D. French, M. Petrie, and K.L. Lynch Role of liquid chromatography-high-resolution mass spectrometry (LC-HR/MS) in clinical toxicology Clin. Toxicol. 50 2012 733 742
3. A. Buchwald, K. Winkler, and T. Epting Validation of an LC-MS/MS method to determine five immunosuppressants with deuterated internal standards including MPA BMC Clin. Pharmacol. 12 2012 6904 6912
4. M.M. Kusunir, A.L. Rockwood, W.L. Roberts, B. Yue, J. Berqquist, and A.W. Meikle Liquid chromatography tandem mass spectrometry for analyses of steroids in clinical laboratories Clin. Biochem. 44 2011 77 88
5. F.G. Strathman, and A.N. Hoofnagle Current and future applications of mass spectrometry to the clinical laboratory Am. J. Clin. Pathol. 136 2011 609 616
6. A. Croxatto, G. Prod'hom, and G. Greub Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology FEMS Microbiol. Rev. 36 2012 380 407
7. A.E. Clark, E.J. Kaleta, A. Arota, and D.M. Wolk Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology Clin. Microbiol. Rev. 26 S 2013 547 603
8. O. Bader MALDI-TOF-MS-based species identification and typing approaches in medical mycology Proteomics 13 2013 788 799
9. S. Biswas, and J.M. Rolain Use of MALDI-TOF mass spectrometry for identification of bacteria that are difficult to culture J. Microbiol. Methods 92 2013 13 24
10. R. Zhang, Y. Long, W. He, X. Hao, and J. Liu Application status of MALDI-TOF mass spectrometry of Mycobacterium tuberculosis J. Thorac. Dis. 6 2014 512 516
11. W.H. Sperber The identification of staphylococci in clinical and food microbiology laboratories CRC Crit. Rev. Clin. Lab. Sci. 7 1976 121 184
12. L.D. Gray, and D.P. Fedorko Laboratory diagnosis of bacterial meningitis Clin. Microbiol. Rev. 5 1992 130 145
13. T. Avni, N. Mansur, L. Leibovici, and M. Paul PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis J. Clin. Microbiol. 48 2010 489 496
14. S.K. Rampini, G.V. Bloemberg, P.M. Keller, A.C. Bucherler, G. Dollenmaier, R.F. Speck, and E.C. Bottger Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial meningitis Clin. Infect. Dis. 53 2011 1245 1251
15. J.P. Anhalt, and G. Fenselau Identification of bacteria using mass spectrometry Anal. Chem. 47 1975 219 225
16. K. Tanaka, and J.B. Fenn Nobel Laureates in chemistry Available at: http://www.nobelprize.org
17. R.D. Holland, J.G. Wilkes, F. Rafii, J.B. Sutherland, C.C. Persons, K.J. Voorhees, and J.O. Lay Jr. Rapid identification of intact whole bacteria on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry Rapid Commun. Mass Spectrom. 10 1996 1227 1232
18. M.A. Claydon, S.N. Davey, V. Edwards-Jones, and D.B. Gordon The rapid identification of intact microorganisms using mass spectrometry Nat. Biotechnol. 14 1996 1584 1586
19. A.M. Haag, S.N. Taylor, K.H. Johnston, and R.B. Cole Rapid identification and speciation of hemophilus bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry J. Mass Spectrom. 33 1998 750 756
20. K.J. Welham, M.A. Domin, D.E. Scannel, E. Cohen, and D.S. Ashton The characterization of micro-organisms by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Rapid Commun. Mass Spectrom. 12 1988 176 180
21. P.A. Demirev, Y.P. Ho, V. Ryzhov, and C. Fenselau Microorganism identification by mass spectrometry and protein database searches Anal. Chem. 71 1999 2732 2738
22. M.A. Wikler, J. Uher, and S. Cepa Direct analysis and identification of Helicobacter and Campylobacter species by MALDI-TOF mass spectrometry Anal. Chem. 71 1999 3416 3419
23. K.H. Jarman, S.T. Cebula, A.J. Saenz, C.E. Petersen, N.B. Valentine, M.T. Kingsley, and K.L. Wahl An algorithm for automated bacterial identification using matrix-assisted laser desorption/ionization mass spectrometry Anal. Chem. 72 2000 1217 1223
24. R.A. Everley, T.M. Mott, S.A. Wyatt, D.M. Toney, and T.R. Croley Liquid chromatography mass spectrometry characterization of Escherichia coli and Shigella species J. Am. Soc. Mass Spectrom. 19 2008 1621 1628
25. M. Welker Proteomics for routine identification of microorganisms Proteomics 11 2011 3143 3153
26. K. Teramoto, H. Sato, L. Sun, M. Torimura, H. Tao, H. Yoshikawa, Y. Hotta, A. Hosoda, and H. Tamura Phylogenetic classification of Pseudomonas putida strains by MALDI-MS using ribosomal subunit proteins as biomarkers Anal. Chem. 79 2007 8712 8719
27. V. Ryzhov, and C. Fenselau Characterization of the protein subset desorbed by MALDI from whole bacterial cells Anal. Chem. 73 2001 746 750
28. E. Carbonnelle, C. Mesquita, E. Bille, N. Day, B. Dauphin, J.L. Beretti, A. Ferroni, L. Gutmann, and X. Nassif MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory Clin. Biochem. 44 2011 104 109
29. N. Valentine, S. Wunschel, D. Wunschel, C. Petersen, and K. Wahl Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry Appl. Environ. Microbiol. 71 71 2005 58 64
30. K. Sogawa, M. Watanabe, K. Sato, S. Segawa, C. Ishii, A. Miyabe, S. Murata, T. Saito, and F. Nomura Use of the MALDI BioTyper system with MALDI-TOF mass spectrometry for rapid identification of microorganisms Anal. Bioanal. Chem. 400 2011 1905 1911
31. B.A. Ford, and C.A. Burham Optimization of routine identification of clinically relevant Gram-negative bacteria by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Bruker Biotyper J. Clin. Microbiol. 51 2013 1412 1420
32. D. Bubois, D. Leyssene, J.P. Chacornac, M. Kostrzewa, P.O. Schmit, R. Talon, R. Bonnet, and J. Delmas Identification of a variety of Staphylococcus species by matrix-assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 48 2010 941 945
33. Y. Hotta, J. Sato, H. Sato, A. Hosoda, and H. Tamura Classification of the genus Bacillus based on MALDI-TOF MS analysis of ribosomal proteins coded in S10 and spc operons J. Agric. Food Chem. 59 2011 5222 5230
34. P. Seng, M. Drancourt, F. Couriet, B. La Scola, P.E. Fournier, J.M. Roalian, and D. Raoult Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry Clin. Infect. Dis. 49 2009 543 551
35. U. Eigner, M. Hofelder, K. Oberdorfer, U. Betz-Wild, D. Bertsch, and A.M. Fahr Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory Clin. Lab. 55 2009 289 296
36. A. Bizzini, C. Durussel, J. Bille, G. Greub, and G. Prod'hom Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory J. Clin. Microbiol. 48 2010 1549 1554
37. K. Sogawa, M. Watanabe, K. Sato, S. Segawa, A. Miyabe, S. Murata, T. Saito, and F. Nomura Rapid identification of microorganisms by mass spectrometry: improved performance by incorporation of in-house spectral data into a commercial database Anal. Bioanal. Chem. 403 2012 1811 1822
38. R. Patel MALDI-TOF mass spectrometry: transformative proteomics for clinical microbiology Clin. Chem. 59 2013 340 342
39. S.Q. van Veen, E.C. Claas, and E.J. Kuijper High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories J. Clin. Microbiol. 48 2010 900 907
40. E. Bessede, M. Angla-Gre, Y. Delagarde, S. Sep Hieng, and F. Megraud Matrix-assisted laser-desorption/ionization biotyper: experience in the routine of a university hospital Clin. Microbiol. Infect. 17 2011 533 538
41. S.A. Neville, A. Lecorider, H. Ziochos, M.J. Chater, I.B. Gosbell, M.W. Maley, and S.J. van Hal Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification J. Clin. Microbiol. 49 2011 2980 2984
42. A. Cherkaoui, J. Hibbs, S. Emonet, M. Tangomo, M. Girard, P. Francois, and J. Schrenzel Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level J. Clin. Microbiol. 48 2010 1169 1175
43. D.C. Marko, R.T. Saffeert, S.A. Cunningham, J. Hyman, J. Walsh, S. Arbefeville, W. Howard, J. Pruessner, N. Safwat, F.R. Cockerill, A.D. Bossler, R. Patel, and S.S. Richter Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients J. Clin. Microbiol. 50 2012 2034 2039
44. D. Martiny, L. Busson, I. Wybo, R.A. El Haj, A. Dediste, and O. Vandenberg Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 50 2012 1313 1325
45. N. Mancini, E. De Carolis, L. Infurmari, A. Vella, N. Clementi, L. Vaccaro, A. Ruggeri, B. Posteraro, R. Burioni, M. Clementi, and M. Sanquinetti Comparative evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance J. Clin. Microbiol. 51 2013 2453 2457
46. J.H. Chen, P.L. Ho, G.S. Kwan, K.K. She, G.K. Siu, V.C. Cheng, K.Y. Yuen, and W.C. Yam Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems J. Clin. Microbiol. 51 2013 1733 1739
47. K. Alby, P.H. Gilligan, and M.B. Miller Comparison of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry platforms for the identification of gram-negative rods from patients with cystic fibrosis J. Clin. Microbiol. 51 2013 3852 3854
48. M.A. Pence, E. McElvania Tekippe, M.A. Wallace, and C.A. Burnham Comparison and optimization of two MALDI-TOF MS platforms for the identification of medically relevant yeast species Eur. J. Clin. Microbiol. Infect. Dis. 33 2014 1703 1712
49. A. Hamprecht, S. Crist, T. Oestreicher, G. Plum, V.A. Kempf, and S. Gottig Performance of two MALDI-TOF MS systems for the identification of yeasts isolated from bloodstream infections and cerebrospinal fluids using a time-saving direct transfer protocol Med. Microbiol. Immunol. 203 2014 93 99
50. P. Karpanoja, I. Haruju, K. Rantakokko-Jalava, M. Haanpera, and H. Sarkkinen Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of viridans group streptococci Eur. J. Clin. Microbiol. Infect. Dis. 33 2014 779 788
51. C.A. Mather, S.F. Rivera, and S.M. Butler-Wu Comparison of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of mycobacteria using simplified extraction protocols J. Clin. Microbiol. 52 2014 130 138
52. M. Welker, and E.R.B. Moore Applications of whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry in systematic microbiology Syst. Appl. Microbiol. 34 2011 2 11
53. P.D. Khot, M.R. Couturier, A. Wilsonm, A. Croft, and M.A. Fisher Optimization of matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis for bacterial identification J. Clin. Microbiol. 50 2012 3845 3852
54. P.D. Khot, and M.A. Fisher Novel approach for differentiating Shigella species and Escherichia coli by matrix assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 51 2013 3711 3716
55. N. Matsuda, M. Matsuda, S. Notake, H. Yokokawa, Y. Kawamura, K. Hiramatsu, and K. Kikuchi Evaluation of a simple protein extraction method for species identification of clinically relevant staphylococci by matrix-assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 50 2012 3862 3868
56. L. Ferreira, F. Sanchez-Juanes, M. Gonzalez-Avilla, D. Cembrero-Fucinos, A. Herrero-Hernandez, J.M. Gonzalez-Buitrago, and J.L. Munoz-Bellido Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 48 2010 2110 2115
57. X.H. Wang, G. Zhang, Y.Y. Fan, X. Yang, W.J. Sui, and X.X. Lu Direct identification of bacteria causing urinary tract infections by combining matrix-assisted laser desorption ionization-time of flight mass spectrometry with UF-1000i urine flow cytometry J. Microbiol. Methods 92 2013 231 235
58. M.L. Demarco, and C.A. Burnham Diafiltration MALDI-TOF mass spectrometry method for culture independent detection and identification of pathogens directly from urine specimens Am. J. Clin. Pathol. 141 2014 204 212
59. S.G. Heckenberg, M.C. Brouwer, and D. van de Beek Bacterial meningitis Handbook Clin. Neurol. 121 2014 1361 1375
60. G. Nyvang Hartmeyer, A. Kvistholm Jensen, S. Bocher, M. Damakjaer Bartels, M. Pedersen, M. Engell Clausen, R. Abdul-Redha, R. Dargis, P. Schouenborg, N. Hojyng, M. Kemp, and J.J. Chistensen Mass spectrometry: pneumococcal meningitis verified and Brucella species identified in less than half an hour Scand. J. Infect. Dis. 42 2010 716 718
61. S. Segawa, S. Sawai, S. Murata, M. Nishimura, M. Beppu, K. Sogawa, M. Watanabe, M. Satoh, T. Matsutani, M. Kobayashi, Y. Iwadate, S. Kuwabara, N. Saeki, and F. Nomura Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis Clin. Chim. Acta 435 2014 59 61
62. A. Kumar, P. Ellis, Y. Arabi, D. Roberts, B. Light, J.E. Parillo, P. Dodek, G. Wood, A. Kumar, D. Simon, C. Peters, M. Ahsan, and D. Chateau Cooperative antimicrobial therapy of septic shock database research group, Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock Chest 136 2009 1237 1248
63. B. La Scola, and D. Raoult Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionization time-of-flight mass spectrometry PLoS One 25 2009 e8041
64. L.G. Stevenson, S.K. Drake, and P.R. Murray Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 48 2010 444 447
65. P.R. Lagace-Wiens, H.J. Adam, J.A. Karlowsky, K.A. Nichol, P.F. Pang, J. Guenther, A.A. Webb, C. Miller, and M.J. Alfa Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time J. Clin. Microbiol. 50 2012 3324 3328
66. E.A. Idelevich, I. Schule, N. Grunastel, J. Wullenweber, G. Peters, and K. Becker Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium Clin. Microbiol. Infect. 2014 (Epub ahead of print)
67. K.E. Tan, B.C. Ellis, R. Lee, P.D. Stamper, S.X. Zhang, and K.C. Carroll Prospective evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness J. Clin. Microbiol. 50 2012 3301 3308
68. K.K. Perez, R.J. Olsen, W.L. Musick, P.L. Cernoch, J.R. Davis, G.A. Land, L.E. Peterson, and J.M. Musser Integrating rapid pathogen identification and antimicrobial stewardship significantly decreases hospital costs Arch. Pathol. Lab. Med. 37 2013 1247 1254
69. T.R. Sandrin, J.E. Goldstein, and S. Schumaker MALDI TOF MS profiling of bacteria at the strain level: a review Mass Spectrom. Rev. 32 2013 188 217
70. M. Vargha, Z. Takats, A. Konopka, and C.H. Nakatsu Optimization of MALDI-TOF MS for strain level differentiation of Arthrobacter isolates J. Microbiol. Methods 66 2006 399 409
71. R. Dieckmann, R. Helmuth, M. Erhard, and B. Malorny Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry Appl. Environ. Microbiol. 74 2008 7767 7778
72. Y. Fujinami, H.S. Kikkawa, Y. Kurosaki, K. Sakurada, M. Yoshino, and J. Yasuda Rapid discrimination of Legionella by matrix-assisted laser desorption ionization time-of-flight mass spectrometry Microbiol. Res. 166 2011 77 86
73. J. Hrabak, E. Chudackova, and R. Walkova Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis Clin. Microbiol. Rev. 26 2013 103 114
74. M. Kostrzewa, K. Sparbier, T. Maier, and S. Schubert MALDI-TOF MS: an upcoming tool for rapid detection of antibiotic resistance in microorganisms Proteomics Clin. Appl. 7 2013 767 778
75. V. Edwards-Jones, M.A. Claydon, D.J. Evason, J. Walker, A.J. Fox, and D.B. Gordon Rapid discrimination between methicillin-sensitive and methicillin resistant Staphylococcus aureus by intact cell mass spectrometry J. Med. Microbiol. 49 2000 295 300
76. J. Walker, A.J. Fox, V. Edwards-Jones, and D.B. Gordon Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: media effects and inter-laboratory reproducibility J. Microbiol. Methods 48 2002 117 126
77. M. Wolters, H. Rohde, T. Maier, C. Belmar-Campos, G. Franke, S. Schepe, M. Aepfelbacher, and M. Christner MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages Int. J. Med. Microbiol. 301 2011 64 68
78. P. Lasch, C. Fleige, M. Stammler, F. Layer, U. Nubel, W. Witte, and G. Werner Insufficient discriminatory power of MALDI-TOF mass spectrometry for typing of Enterococcus faecium and Staphylococcus aureus isolates J. Microbiol. Methods 100 2014 58 69
79. K. Sparbier, C. Lange, J. Jung, A. Wieser, S. Schubert, and M. Kostrzewa MALDI biotyper-based rapid resistance detection by stable-isotope labeling J. Clin. Microbiol. 51 2013 3741 3748
80. J. Hrabak, R. Walkova, V. Studentova, E. Chudackova, and T. Bergerova Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry J. Clin. Microbiol. 49 2011 3222 3227
81. K. Sparbier, S. Schbert, U. Welleer, C. Boogen, and M. Kostrzewa Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics 50 2012 927 937
82. M. Oviano, B. Fernandez, A. Fernandez, M.J. Barba, C. Mourino, and G. Bou Rapid detection of enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry Clin. Microbiol. Infect. 2014 (Epub ahead of print)
83. P.M. Griffin, G.R. Price, J.M. Schooneveldt, S. Schoebusch, M.H. Tilse, T. Urbanski, B. Hamilton, and D. Venter Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak J. Clin. Microbiol. 50 2012 2918 2931
84. J. Intelicato-Young, and A. Fox Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria J. Microbiol. Methods 92 2013 381 386
85. S.I. Alam, B. Kumar, and D.V. Kamboj Multiplex detection of protein toxins using MALDI-TOF-TOF tandem mass spectrometry: application in unambiguous toxin detection from bioaerosol Anal. Chem. 84 2012 10500 10507
86. C.K. Fagerquist, W.J. Zaragoza, O. Sultan, N. Woo, B. Quinones, M.B. Cooley, and R.E. Mandrell Top-down proteomic identification of Shiga toxin 2 subtypes from Shiga toxin-producing Escherichia coli by matrix-assisted laser desorption ionization-tandem time of flight mass spectrometry Appl. Environ. Microbiol. 80 2014 2928 2940
87. J.P. Charrier Rapid screening of antibiotic resistance mechanisms and virulence factors: new mass spectrometry applications for clinical use? The 5th Annual Conference & Exhibits of Mass Spectrometry: Applications to the Clinical Lab, San Diego, CA February, 2013
88. A. Burillo, B. Rodriguez-Sanchez, A. Ramiro, E. Cercenado, M. Rodriguez-Creixems, and E. Bouza Gram-stain plus MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) for a rapid diagnosis of urinary tract infection PLoS One 9 2014 e86915