Gram-stain plus MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry) for a rapid diagnosis of urinary tract infection

PLoS ONE

Volumn 9, Issue 1, 2014, Pages

  Burillo, Almudena ^a,b   Rodríguez Sánchez, Belén ^a,d   Ramiro, Ana ^a   Cercenado, Emilia ^a,b,c,d   Rodríguez Créixems, Marta ^a,c,d   Bouza, Emilio ^a,b,c,d  

^a HOSPITAL GENERAL UNIVERSITARIO GREGORIO MARAÑÓN   (Spain)

^b UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^c Red Española de Investigación en Patología Infecciosa REIPI RD06 0008 1025   (Spain)

^d INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT;

ADULT; AGED; ALGORITHM; ANALYTICAL ERROR; ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; FEASIBILITY STUDY; FEMALE; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GRAM STAINING; HUMAN; KLEBSIELLA PNEUMONIAE; MAJOR CLINICAL STUDY; MALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROBIAL MORPHOLOGY; NONHUMAN; PREDICTIVE VALUE; SENSITIVITY AND SPECIFICITY; TIME TO TREATMENT; TURNAROUND TIME; URINALYSIS; URINARY TRACT INFECTION; URINE CULTURE; YEAST;

ALGORITHMS; GENTIAN VIOLET; HUMANS; LIKELIHOOD FUNCTIONS; PHENAZINES; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; URINARY TRACT INFECTIONS; URINE;

EID: 84899504756     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0086915     Document Type: Article

References (45)

1. Foxman B (2010) The epidemiology of urinary tract infection. Nat Rev Urol 7: 653-660.
2. McIsaac WJ, Hunchak CL (2011) Overestimation error and unnecessary antibiotic prescriptions for acute cystitis in adult women. Med Decis Making 31: 405-411.
3. Staneck JL (1985) Screening tests and "rapid" identification. Is anybody out there listening? Diagn Microbiol Infect Dis 3: 51S-57S.
4. Foxman B, Brown P (2003) Epidemiology of urinary tract infections: transmission and risk factors, incidence, and costs. Infect Dis Clin North Am 17: 227-241. (Pubitemid 36760181)
5. Lewis JF, Alexander J (1976) Microscopy of stained urine smears to determine the need for quantitative culture. J Clin Microbiol 4: 372-374.
6. Clarridge JE, Johnson JR, Pezzlo MT (1998) Cumitech 2B, Laboratory diagnosis of urinary tract infections; Welssfeld AS, editor. Washington, D. C.: American Society for Microbiology. 21 p.
7. Ho YP, Reddy PM (2011) Advances in mass spectrometry for the identification of pathogens. Mass Spectrom Rev 30: 1203-1224.
8. Cherkaoui A, Hibbs J, Emonet S, Tangomo M, Girard M, et al. (2010) Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol 48: 1169-1175.
9. La Scola B, Raoult D (2009) Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PloS One 4: e8041.
10. Ferroni A, Suarez S, Beretti JL, Dauphin B, Bille E, et al. (2010) Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48: 1542-1548.
11. Vlek AL, Bonten MJ, Boel CH (2012) Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia. PloS One 7: e32589.
12. Ferreira L, Sanchez-Juanes F, Gonzalez-Avila M, Cembrero-Fucinos D, Herrero-Hernandez A, et al. (2010) Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48: 2110-2115.
13. Ferreira L, Sanchez-Juanes F, Munoz-Bellido JL, Gonzalez-Buitrago JM (2011) Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method. Clin Microbiol Infect 17: 1007-1012.
14. Kohling HL, Bittner A, Muller KD, Buer J, Becker M, et al. (2012) Direct identification of bacteria in urine samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and relevance of defensins as interfering factors. J Med Microbiol 61: 339-344.
15. Wang XH, Zhang G, Fan YY, Yang X, Sui WJ, et al. (2013) Direct identification of bacteria causing urinary tract infections by combining matrix-assisted laser desorption ionization-time of flight mass spectrometry with UF-1000i urine flow cytometry. J Microbiol Methods 92: 231-235.
16. Pezzlo M, York MK, Church DL (2010) Urine cultures. In: Garcia LS, editor. Clinical Procedures Handbook. 3rd ed. Washington, D. C.: ASM Press. 3.12.11-13.12.31.
17. Bouza E, San Juan R, Munoz P, Voss A, Kluytmans J (2001) A European perspective on nosocomial urinary tract infections I. Report on the microbiology workload, etiology and antimicrobial susceptibility (ESGNI-003 study). European Study Group on Nosocomial Infections. Clin Microbiol Infect 7: 523-531. (Pubitemid 34960342)
18. Sundqvist M, Kahlmeter G (2009) 'Pre-emptive culturing' will improve the chance of 'getting it right' when empirical therapy of urinary tract infections fails. J Antimicrob Chemother 64: 227-228.
19. Deville WL, Yzermans JC, van Duijn NP, Bezemer PD, van der Windt DA, et al. (2004) The urine dipstick test useful to rule out infections. A meta-analysis of the accuracy. BMC Urol 4: 4.
20. Wilson ML, Gaido L (2004) Laboratory diagnosis of urinary tract infections in adult patients. Clin Infect Dis 38: 1150-1158. (Pubitemid 38951264)
21. St John A, Boyd JC, Lowes AJ, Price CP (2006) The use of urinary dipstick tests to exclude urinary tract infection: a systematic review of the literature. Am J Clin Pathol 126: 428-436. (Pubitemid 44338797)
22. Williams GJ, Macaskill P, Chan SF, Turner RM, Hodson E, et al. (2010) Absolute and relative accuracy of rapid urine tests for urinary tract infection in children: a meta-analysis. Lancet Infect Dis10: 240-250.
23. Goulden BE (1968) Assessment of the usefulness of the examination of a gram smear of fresh uncentrifuged urine in the determination of significant bacteriuria in dogs. N Z Vet J 16: 1-2.
24. Jorgensen JH, Jones PM (1975) Comparative evaluation of the Limulus assay and the direct Gram stain for detection of significant bacteriuria. Am J Clin Pathol 63: 142-148.
25. Robins DG, Rogers KB, White RH, Osman MS (1975) Urine microscopy as an aid to detection of bacteriuria. Lancet 1: 476-478.
26. Crout FV, Tilton RC (1984) Rapid screening of urine for significant bacteriuria by Gram stain, acridine orange stain, and the Autobac MTS system. Diagn Microbiol Infect Dis 2: 179-186. (Pubitemid 15191784)
27. Tilton RE, Tilton RC (1980) Automated direct antimicrobial susceptibility testing of microscopically screened urine cultures. J Clin Microbiol 11: 157-161. (Pubitemid 10169132)
28. Wiwanitkit V, Udomsantisuk N, Boonchalermvichian C (2005) Diagnostic value and cost utility analysis for urine Gram stain and urine microscopic examination as screening tests for urinary tract infection. Urol Res 33: 220-222. (Pubitemid 40884038)
29. Kwon JH, Fausone MK, Du H, Robicsek A, Peterson LR (2012) Impact of laboratory-reported urine culture colony counts on the diagnosis and treatment of urinary tract infection for hospitalized patients. Am J Clin Pathol 137: 778-784.
30. Kuijper EJ, van der Meer J, de Jong MD, Speelman P, Dankert J (2003) Usefulness of Gram stain for diagnosis of lower respiratory tract infection or urinary tract infection and as an aid in guiding treatment. Eur J Clin Microbiol Infect Dis 22: 228-234. (Pubitemid 36578908)
31. Spanu T, Posteraro B, Fiori B, D'Inzeo T, Campoli S, et al. (2012) Direct malditof mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infections: an observational study in two large microbiology laboratories. J Clin Microbiol 50: 176-179.
32. Rodríguez-Sánchez B, Sánchez-Carrillo C, Ruiz A, Rodríguez-Créixems M, Bouza E (2012) Rapid identification of pathogens by MALDI-TOF MS in blood culture bottles. In: American Society of Microbiology, editor. Interscience Conference on Antimicrobial Agents and Chemotherapy. San Francisco, 9-12 September, 2012. pp. D-726.
33. Bjørnholt JV, Nilsen SM, Noorland I, Wigemyr M, Løken CH, et al. (2011) MALDI-TOF mass spectrometry ID of bacteria directly from cerebrospinal fluid: what you see is what you get. Clin Microbiol Infect 17: S66.
34. Christner M, Rohde H, Wolters M, Sobottka I, Wegscheider K, et al. (2010) Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting. J Clin Microbiol 48: 1584-1591.
35. Kulkarni S, Nigrin J (2013) Letter to the Editor re: "Performance of flow cytometry to screen urine for bacteria and white blood cells prior to urine culture". Clin Biochem. doi: 10.1016/j.clinbiochem.2013.09.010.
36. Hartley S, Valley S, Kuhn L, Washer LL, Gandhi T, et al. (2013) Inappropriate testing for urinary tract infection in hospitalized patients: an opportunity for improvement. Infect Control Hosp Epidemiol 34: 1204-1207.
37. Rodriguez-Sanchez B, Sanchez-Carrillo C, Ruiz A, Marin M, Cercenado E, et al. (2013) Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Clin Microbiol Infect. Epub ahead of print.
38. Loonen AJ, Jansz AR, Stalpers J, Wolffs PF, van den Brule AJ (2012) An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 31: 1575-1583.
39. Hoyos-Mallecot Y, Miranda-Casas C, Cabrera-Alvargonzalez JJ, Gomez-Camarasa C, Perez-Ramirez MD, et al. (2013) [Bacterial identification from blood cultures by a rapid Matrix-Assisted Laser Desorption-Ionisation Time-of-Flight mass spectrometry technique]. Enferm Infecc Microbiol Clin 31: 152-155.
40. Cheng JW, Tang YE, Jureen R, Lin RTP, Teo JWP (2013) Modified protocol for yeast identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. AJMR American Journal of Microbiological Research. pp. 71-73.
41. Kallenius G, Dornbusch K, Hallander HO, Jakobsson K (1981) Comparison of direct and standardized antibiotic susceptibility testing in bacteriuria. Chemother 27: 99-105. (Pubitemid 11194492)
42. Bronnestam R (1999) Direct antimicrobial susceptibility testing in bacteriuria. APMIS 107: 437-444.
43. Burd EM, Kehl KS (2011) A critical apparisal of the role of the clinical microbiology laboratory in the diagnosis of urinary tract infections. J Clin Microbiol 49: S34-S38.
44. Clerc O, Prod'hom G, Vogne C, Bizzini A, Calandra T, et al. (2013) Impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry on the clinical management of patients with Gram-negative bacteremia: a prospective observational study. Clin Infect Dis 56: 1101-1107.
45. Huang AM, Newton D, Kunapuli A, Gandhi TN, Washer LL, et al. (2013) Impact of rapid organism identification via matrix-assisted laser desorption/ionization time-of-flight combined with antimicrobial stewardship team intervention in adult patients with bacteremia and candidemia. Clin Infect Dis 57: 1237-1245.