Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems

Journal of Clinical Microbiology

Volumn 51, Issue 6, 2013, Pages 1733-1739

  Chen, Jonathan H K ^a   Ho, Pak Leung ^a   Kwan, Grace S W ^a   She, Kevin K K ^a   Siu, Gilman K H ^a   Cheng, Vincent C C ^a,b   Yuen, Kwok Yung ^a   Yam, Wing Cheong ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b QUEEN MARY HOSPITAL   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACILLUS; BACILLUS CEREUS; BACILLUS CIRCULANS; BACILLUS HORNECKIAE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BREVUNDIMONAS DIMINUTA; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC KIT; INTERMETHOD COMPARISON; MASS SPECTROMETER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; RNA GENE; SALMONELLA TYPHI; STAPHYLOCOCCUS EPIDERMIDIS; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS BOVIS; STREPTOCOCCUS CONSTELLATUS; STREPTOCOCCUS GALLOLYTICUS; STREPTOCOCCUS SANGUINIS; VALIDATION PROCESS;

BACTEREMIA; BACTERIA; BACTERIOLOGICAL TECHNIQUES; COSTS AND COST ANALYSIS; HUMANS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

BACTERIA (MICROORGANISMS); FUNGI;

EID: 84878496270     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03259-12     Document Type: Article

References (28)

1. Ho PL, Chan WM, Tsang KW, Wong SS, Young K. 2002. Bacteremia caused by Escherichia coli producing extended-spectrum beta-lactamase: a case-control study of risk factors and outcomes. Scand. J. Infect. Dis. 34:567-573. (Pubitemid 35012337)
2. Ho PL, Que TL, Ng TK, Chiu SS, Yung RW, Tsang KW. 2006. Clinical outcomes of bacteremic pneumococcal infections in an area with high resistance. Eur. J. Clin. Microbiol. Infect. Dis. 25:323-327.
3. Seifert H. 2009. The clinical importance of microbiological findings in the diagnosis and management of bloodstream infections. Clin. Infect. Dis. 48(Suppl 4):S238-S245.
4. Vlek AL, Bonten MJ, Boel CH. 2012. Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia. PLoS One 7:e32589. doi:10.1371/journal. pone.0032589.
5. Versalovic J, Carroll KC, Funke G, Jorfensen JH, Landry ML, Warnock DW (ed). 2011. Manual of clinical microbiology, 10th ed. ASM Press, Washington, DC.
6. Peters RP, Savelkoul PH, Simoons-Smit AM, Danner SA, Vandenbroucke-Grauls CM, van Agtmael MA. 2006. Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J. Clin. Microbiol. 44:119-123. (Pubitemid 43100589)
7. Karumaa S, Karpanoja P, Sarkkinen H. 2012. PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory. J. Clin. Microbiol. 50:1031-1033.
8. Tan KE, Ellis BC, Lee R, Stamper PD, Zhang SX, Carroll KC. 2012. Prospective evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness. J. Clin. Microbiol. 50:3301-3308.
9. Klein S, Zimmermann S, Kohler C, Mischnik A, Alle W, Bode KA. 2012. Integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in blood culture diagnostics: a fast and effective approach. J. Med. Microbiol. 61:323-331.
10. Spanu T, Posteraro B, Fiori B, D'Inzeo T, Campoli S, Ruggeri A, Tumbarello M, Canu G, Trecarichi EM, Parisi G, Tronci M, Sanguinetti M, Fadda G. 2012. Direct maldi-tof mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infections: an observational study in two large microbiology laboratories. J. Clin. Microbiol. 50:176-179.
11. Stevenson LG, Drake SK, Murray PR. 2010. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 48:444-447.
12. Wuppenhorst N, Consoir C, Lorch D, Schneider C. 2012. Direct identification of bacteria from charcoal-containing blood culture bottles using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Eur. J. Clin. Microbiol. Infect. Dis. 31:2843-2850.
13. Yan Y, He Y, Maier T, Quinn C, Shi G, Li H, Stratton CW, Kostrzewa M, Tang YW. 2011. Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system. J. Clin. Microbiol. 49:2528-2532.
14. Kok J, Thomas LC, Olma T, Chen SC, Iredell JR. 2011. Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper and time of flight mass spectrometry. PLoS One 6:e23285. doi:10.1371/journal.pone.0023285.
15. Janda JM, Abbott SL. 2007. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J. Clin. Microbiol. 45:2761-2764. (Pubitemid 47417311)
16. Woo PC, Teng JL, Yeung JM, Tse H, Lau SK, Yuen KY. 2011. Automated identification of medically important bacteria by 16S rRNA gene sequencing using a novel comprehensive database, 16SpathDB. J. Clin. Microbiol. 49:1799-1809.
17. Martiny D, Dediste A, Vandenberg O. 2012. Comparison of an in-house method and the commercial Sepsityper kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption- ionisation time-of-flight mass spectrometry. Eur. J. Clin. Microbiol. Infect. Dis. 31:2269-2281.
18. Bizzini A, Greub G. 2010. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin. Microbiol. Infect. 16:1614-1619.
19. Mellmann A, Cloud J, Maier T, Keckevoet U, Ramminger I, Iwen P, Dunn J, Hall G, Wilson D, Lasala P, Kostrzewa M, Harmsen D. 2008. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J. Clin. Microbiol. 46:1946-1954. (Pubitemid 351831825)
20. Saffert RT, Cunningham SA, Ihde SM, Jobe KE, Mandrekar J, Patel R. 2011. Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of Gram-negative bacilli. J. Clin. Microbiol. 49:887-892.
21. Martiny D, Busson L, Wybo I, El Haj RA, Dediste A, Vandenberg O. 2012. Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 50:1313-1325.
22. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-offlight mass spectrometry. Clin. Infect. Dis. 49:543-551.
23. Ho PL, Johnson DR, Yue AW, Tsang DN, Que TL, Beall B, Kaplan EL. 2003. Epidemiologic analysis of invasive and noninvasive group a streptococcal isolates in Hong Kong. J. Clin. Microbiol. 41:937-942. (Pubitemid 36307089)
24. Ho PL, Shek RH, Chow KH, Duan RS, Mak GC, Lai EL, Yam WC, Tsang KW, Lai WM. 2005. Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002. J. Antimicrob. Chemother. 55:326-332. (Pubitemid 40425457)
25. Kang CI, Kim SH, Park WB, Lee KD, Kim HB, Kim EC, Oh MD, Choe KW. 2005. Bloodstream infections caused by antibiotic-resistant Gram-negative bacilli: risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome. Antimicrob. Agents Chemother. 49:760-766.
26. Kim SH, Park WB, Lee CS, Kang CI, Bang JW, Kim HB, Kim NJ, Kim EC, Oh MD, Choe KW. 2006. Outcome of inappropriate empirical antibiotic therapy in patients with Staphylococcus aureus bacteraemia: analytical strategy using propensity scores. Clin. Microbiol. Infect. 12:13-21. (Pubitemid 43372234)
27. Lee CC, Lee CH, Chuang MC, Hong MY, Hsu HC, Ko WC. 2012. Impact of inappropriate empirical antibiotic therapy on outcome of bacteremic adults visiting the ED. Am. J. Emerg. Med. 30:1447-1456.
28. Tang WM, Ho PL, Fung KK, Yuen KY, Leong JC. 2001. Necrotising fasciitis of a limb. J. Bone Joint Surg. Brit. 83:709-714. (Pubitemid 32700676)