Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: Validation using digital real-time RT-LAMP

Nucleic Acids Research

Volumn 44, Issue 2, 2016, Pages e10-

  Khorosheva, Eugenia M ^a   Karymov, Mikhail A ^a   Selck, David A ^a   Ismagilov, Rustem F ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; VIRUS RNA; 5' UNTRANSLATED REGION; PRIMER DNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; CLINICAL EFFECTIVENESS; CONTROLLED STUDY; DIGITAL IMAGING; DNA FRAGMENTATION; ENZYME ACTIVITY; HEPATITIS C VIRUS; IMAGE ANALYSIS; LIMIT OF DETECTION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; VALIDATION PROCESS; CHEMISTRY; DEVICES; GENETICS; HEPACIVIRUS; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SYNTHESIS; TIME FACTOR; VALIDATION STUDY;

5' UNTRANSLATED REGIONS; DNA PRIMERS; HEPACIVIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 84961231915     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkv877     Document Type: Article

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