Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

Nature Protocols

Volumn 3, Issue 5, 2008, Pages 877-882

  Tomita, Norihiro ^a   Mori, Yasuyoshi ^a   Kanda, Hidetoshi ^a   Notomi, Tsugunori ^a  

^a EIKEN CHEMICAL CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CALCEIN; MANGANESE; METAL ION; PYROPHOSPHATE; SODIUM CHLORIDE;

ARTICLE; BASIC RESEARCH; DNA SYNTHESIS; FLUORESCENCE; GENE SEQUENCE; GENETIC SCREENING; HUMAN GENOME; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MEDICINE; NUCLEIC ACID AMPLIFICATION; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; PHARMACY; POINT OF CARE TESTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TIME;

DIPHOSPHATES; DNA; DNA PRIMERS; FLUORESCEINS; GENOMICS; HUMANS; MANGANESE; METALS; NUCLEIC ACID AMPLIFICATION TECHNIQUES;

EID: 43149084823     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.57     Document Type: Article

References (19)

1. Saiki, R.K. et al. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350-1354 (1985).
2. Higuchi, R., Dollinger, G., Walsh, P.S. & Griffith, R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology (NY) 10, 413-417 (1992).
3. Higuchi, R., Fockler, C., Dollinger, G. & Watson, R. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions. Biotechnology (NY) 11, 1026-1030 (1993).
4. Holland, P.M., Abramson, R.D., Watson, R. & Gelfand, D.H. Detection of specific polymerase chain reaction product by utilizing the 5′→-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 7276-7280 (1991).
5. Tyagi, S. & Kramer, F.R. Molecular beacons: Probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303-308 (1996).
6. Whitcombe, D., Theaker, J., Guy, S.P., Brown, T. & Little, S. Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 17, 804-807 (1999).
7. Zhang, D.Y., Brandwein, M., Hsuih, T.C. & Li, H. Amplification of target-specific, ligation-dependent circular probe. Gene 211 277-285 (1998).
8. Compton, J. Nucleic acid sequence-based amplification. Nature 350, 91-92 (1991).
9. Guatelli, J.C. et al. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proc. Natl. Acad. Sci. USA 87, 1874-1878 (1990).
10. Walker, G.T. et al. Strand displacement amplification - an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 20, 1691-1696 (1992).
11. Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, e63 (2000).
12. Fukuta, S. et al. Detection of Japanese yam mosaic virus by RT-LAMP. Archives of Virology 148, 1713-1720 (2003).
13. Mori, Y., Nagamine, K., Tomita, N. & Notomi, T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289, 150-154 (2001).
14. Diehl, H. & Ellingboe, J.L. Indicator for titration of calcium in presence of magnesium using disodium dihydrogen ethylenediamine tetraacetate. Anal. Chem. 28, 882-884 (1956).
15. Kepner, B.L. & Hercules, D.M. Fluorometric determination of calcium in blood serum. Anal. Chem. 35, 1238-1240 (1963).
16. Hoelzl Wallach, D.F. & Steck, T.L. Fluorescence techniques in the microdetermination of metals in biological materials. Utility of 2, 4-bis-(N,N-di-(carboxymethyl)aminomethyl) fluorescein in the fluorometric estimation of Al+3, alkaline earths, Co+2, Cu+2, Ni+2, and Zn+2 in micromolar concentrations. Anal. Chem. 35, 1035-1044 (1963).
17. Körbl, J. & Vydra, F. Metallochromic indicators. IV. A note on the preparation and properties of "Calcein". Collect. Czech. Chem. Commun. 23, 622-627 (1958).
18. Demertzis, M.A. Fluorimetric determination of calcium in serum with calcein: Complexation of calcein with calcium and alkali metals. Anal. Chim. Acta. 209, 303-308 (1988).
19. Nagamine, K., Watanabe, K., Ohtsuka, K., Hase, T. & Notomi, T. Loop-mediated isothermal amplification reaction using a nondenatured template. Clin. Chem. 47, 1742-1743 (2001).