Investigation of the molecular mechanism of ICAN, a novel gene amplification method

Journal of Biochemistry

Volumn 142, Issue 2, 2007, Pages 283-292

  Uemori, Takashi ^a   Mukai, Hiroyuki ^a   Takeda, Osamu ^a   Moriyama, Mariko ^a,b   Sato, Yoshimi ^a   Hokazono, Shigekazu ^a   Takatsu, Nariaki ^a   Asada, Kiyozo ^a   Kato, Ikunoshin ^a  

^a TAKARA BIO INC   (Japan)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

ICAN; Multi priming; RNaseH; Strand displacement; Template switching

Indexed keywords

DNA POLYMERASE; PRIMER DNA; RIBONUCLEASE H;

ARTICLE; DNA STRAND; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME STABILITY; GENE AMPLIFICATION; ISOTHERMAL AND CHIMERIC PRIMER INITIATED AMPLIFICATION OF NUCLEIC ACID; NUCLEOTIDE SEQUENCE; REACTION ANALYSIS; THERMOSTABILITY;

DNA BREAKS, SINGLE-STRANDED; DNA PRIMERS; MODELS, BIOLOGICAL; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RIBONUCLEASE H;

EID: 35748983857     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvm137     Document Type: Article

References (4)

1. Mukai, H., Uemori, T., Takeda, O., Kobayashi, E., Yamamoto, J., Nishiwaki, K., Enoki, T., Sagawa, H., Asada, K., and Kato, I. (2007) Highly efficient isothermal DNA amplification system using three elements of 5′-DNA-RNA-3′ chimeric primers, RNaseH and strand-displacing DNA polymerase. J. Biochem. doi:10.1093/jb/mvm138
2. AbouHaidar, M.G. and Ivanov, I.G. (1999) Non-enzymatic RNA hydrolysis promoted by the combined catalytic activity of buffers and magnesium ions. Verlag der Zeitschrift fur Naturforschung 54, 542-548
3. Odelberg, S.J., Weiss, R.B., Hata, A., and White, R. (1995) Template-switching during DNA synthesis by Thermus aquaticus DNA polymerase I. Nucleic Acids Res. 23, 2049-2057
4. Innis, M.A. and Gelfand, D.H. (1990) Optimization of PCRs in PCR Protocols (Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J., eds.)pp. 3-12 Academic Press, New York