Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity

Methods in Enzymology

Volumn 573, Issue , 2016, Pages 365-385

  Liu, M Y ^a   Denizio, J E ^a   Kohli, R M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

5 Hydroxymethylcytosine; DNA demethylation; DNA methylation; DNA oxidation; Dot blot; Nucleic acid modification; Nucleoside mass spectrometry; TET family enzymes

Indexed keywords

5 HYDROXYMETHYLCYTOSINE; 5 METHYLCYTOSINE; CELL DNA; CYTOSINE; ENZYME; GENOMIC DNA; NUCLEOSIDE; OLIGONUCLEOTIDE; TEN ELEVEN TRANSLOCATION ENZYME; UNCLASSIFIED DRUG; 5-HYDROXYMETHYLCYTOSINE; DNA; DNA BINDING PROTEIN; MIXED FUNCTION OXIDASE; ONCOPROTEIN; RECOMBINANT PROTEIN; TET1 PROTEIN, HUMAN; TET2 PROTEIN, HUMAN;

ASSAY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IN VITRO STUDY; INSECT CELL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SCINTILLATION COUNTING; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; ANALOGS AND DERIVATIVES; ANIMAL; CELL LINE; CHEMISTRY; DNA METHYLATION; ENZYME ASSAY; IMMUNOBLOTTING; INSECT; METABOLISM; OXIDATION REDUCTION REACTION; PROCEDURES;

5-METHYLCYTOSINE; ANIMALS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; DNA METHYLATION; DNA-BINDING PROTEINS; ENZYME ASSAYS; HUMANS; IMMUNOBLOTTING; INSECTS; MIXED FUNCTION OXYGENASES; NUCLEOSIDES; OLIGONUCLEOTIDES; OXIDATION-REDUCTION; PROTO-ONCOGENE PROTEINS; RECOMBINANT PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 84960157832     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.12.006     Document Type: Chapter

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