Biochemical characterization of a Naegleria TET-like oxygenase and its application in single molecule sequencing of 5-methylcytosine

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 14, 2015, Pages 4316-4321

  Pais, June E ^a   Dai, Nan ^a   Tamanaha, Esta ^a   Vaisvila, Romualdas ^a   Fomenkov, Alexey I ^a   Bitinaite, Jurate ^a   Sun, Zhiyi ^a   Guan, Shengxi ^a   Corrêa, Ivan R ^a   Noren, Christopher J ^a   Cheng, Xiaodong ^b   Roberts, Richard J ^a   Zheng, Yu ^a   Saleh, Lana ^a   Rao, Anjana ^c  

^a Research Department Federal Reserve Bank of Atlanta   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY   (United States)

Author keywords

5 methylcytosine; Bacterial methylome; NgTET1; SMRT sequencing; TET proteins

Indexed keywords

5 METHYLCYTOSINE; BINDING PROTEIN; DNA POLYMERASE; J BINDING PROTEIN; OXYGENASE; TEN ELEVEN TRANSLOCATION LIKE OXYGENASE; THYMIDINE; UNCLASSIFIED DRUG; CYTOSINE; DNA; DNA BINDING PROTEIN; MIXED FUNCTION OXIDASE; ONCOPROTEIN; OXYGEN; PROTOZOAL PROTEIN; TET1 PROTEIN, HUMAN;

ALGORITHM; ANIMAL CELL; ARTICLE; BACTERIAL GENOME; BIOCHEMISTRY; CHEMICAL REACTION KINETICS; CRYSTAL STRUCTURE; EMBRYO; EMBRYONIC STEM CELL; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENE MAPPING; GENE SEQUENCE; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; HYDROGEN BOND; HYDROXYLATION; IN VITRO STUDY; METHYLATION; MOLECULAR RECOGNITION; MOUSE; MUTAGENESIS; NAEGLERIA; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SINGLE MOLECULE REAL TIME SEQUENCING; ANIMAL; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; EPIGENETICS; GENETIC EPIGENESIS; MUTATION; PHYLOGENY;

BACTERIA (MICROORGANISMS); MAMMALIA; NAEGLERIA; NAEGLERIA GRUBERI; PROTISTA; VERTEBRATA;

5-METHYLCYTOSINE; ALGORITHMS; ANIMALS; CYTOSINE; DNA; DNA-BINDING PROTEINS; EPIGENESIS, GENETIC; EPIGENOMICS; HUMANS; MICE; MIXED FUNCTION OXYGENASES; MUTATION; NAEGLERIA; OXYGEN; OXYGENASES; PHYLOGENY; PROTO-ONCOGENE PROTEINS; PROTOZOAN PROTEINS; SEQUENCE ANALYSIS, DNA; THYMIDINE;

EID: 84929484734     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1417939112     Document Type: Article

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