An effective thiol-reactive probe for differential scanning fluorimetry with a standard real-time polymerase chain reaction device

Analytical Biochemistry

Volumn 499, Issue , 2016, Pages 63-65

  Hofmann, Lukas ^a   Gulati, Sahil ^a   Sears, Avery ^a   Stewart, Phoebe L ^a   Palczewski, Krzysztof ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

BODIPY FL l cystine; Differential scanning fluorimetry; High throughput assay; Membrane proteins; Standard RT PCR device; Thiol reactive fluorescent probe

Indexed keywords

BIOMATERIALS; CYSTINES; FLUORESCENCE; HYDROPHOBICITY; PROBES; PROTEINS; SCANNING;

DIFFERENTIAL SCANNING FLUORIMETRY; FLUORESCENT PROBES; HIGH-THROUGHPUT ASSAYS; L-CYSTINE; MEMBRANE PROTEINS;

POLYMERASE CHAIN REACTION;

BORON; DIMETHYL SULFOXIDE; LYSOZYME; RHODOPSIN; THIOL; BODIPY-FL L-CYSTINE; BORON DERIVATIVE; CYSTINE; FLUORESCENT DYE; PROTEIN; THIOL DERIVATIVE;

ARTICLE; DIFFERENTIAL SCANNING FLUOROMETRY; FLUOROMETRY; HYDROPHILICITY; HYDROPHOBICITY; LIGAND BINDING; MELTING POINT; PH; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; PROTEIN STABILITY; REAL TIME POLYMERASE CHAIN REACTION; RETINA ROD OUTER SEGMENT; SUCROSE GRADIENT; ANALOGS AND DERIVATIVES; CHEMICAL PHENOMENA; CHEMISTRY; GENETICS; STANDARDS; TEMPERATURE;

BORON COMPOUNDS; CYSTINE; FLUORESCENT DYES; FLUOROMETRY; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; SULFHYDRYL COMPOUNDS; TEMPERATURE;

EID: 84959126059     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2016.01.016     Document Type: Article

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