Buffer optimization of thermal melt assays of plasmodium proteins for detection of small-molecule ligands

Journal of Biomolecular Screening

Volumn 14, Issue 6, 2009, Pages 700-707

  Crowther, Gregory J ^a   Napuli, Alberto J ^b   Thomas, Andrew P ^a   Chung, Diana J ^a   Kovzun, Kuzma V ^a   Leibly, David J ^b   Castaneda, Lisa J ^b   Bhandari, Janhavi ^b   Damman, Christopher J ^a   Hui, Raymond ^c   Hol, Wim G J ^b   Buckner, Frederick S ^a   Verlinde, Christophe L M J ^b   Zhang, Zhongsheng ^b   Fan, Erkang ^b   Van Voorhis, Wesley C ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

Protein stabilization; Protein unfolding; Superoxide dismutase; Thermal shift assays

Indexed keywords

6 PHOSPHOGLUCONOLACTONASE; 6 PYRUVOYLTETRAHYDROPTERINE SYNTHASE; ADENOSINE DEAMINASE; ADENOSYLHOMOCYSTEINASE; ADENYLOSUCCINATE LYASE; ADENYLOSUCCINATE SYNTHASE; ASPARTATE CARBAMOYLTRANSFERASE; BUFFER; CDK RELATED PROTEIN KINASE 6; CHOLINE KINASE; CYCLOPHILIN; DEXTRO RIBULOSE 5 PHOSPHATE 3 EPIMERASE; DIHYDROOROTATE DEHYDROGENASE; DYNEIN LIGHT CHAIN 1; FARNESYL PYROPHOSPHATE SYNTHASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INITIATION FACTOR 5A; LIGAND; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN; RIBOSEPHOSPHATE ISOMERASE; RIBOSOMAL RNA METHYLTRANSFERASE; SPERMIDINE SYNTHASE; SUPEROXIDE DISMUTASE; THIOREDOXIN; THIOREDOXIN REDUCTASE; TRYPTOPHAN TRANSFER RNA LIGASE; UBIQUITIN CONJUGATING ENZYME; UNCLASSIFIED DRUG; UNINDEXED DRUG; URIDINE PHOSPHORYLASE;

ARTICLE; HIGH THROUGHPUT SCREENING; MALARIA; MELTING POINT; NONHUMAN; PLASMODIUM; PLASMODIUM BERGHEI; PLASMODIUM KNOWLESI; PLASMODIUM VINCKEI; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN STABILITY; TEMPERATURE MEASUREMENT;

ANIMALS; BIOLOGICAL ASSAY; BUFFERS; LIGANDS; PLASMODIUM; PROTOZOAN PROTEINS; SMALL MOLECULE LIBRARIES; TRANSITION TEMPERATURE;

PLASMODIUM PARASITES;

EID: 67651042924     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057109335749     Document Type: Article

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