Thermal destabilization of rhodopsin and opsin by proteolytic cleavage in bovine rod outer segment disk membranes

Biochemistry

Volumn 40, Issue 37, 2001, Pages 11176-11183

  Landin, J S ^a   Katragadda, M ^a   Albert, A D ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DENATURATION;

ACTIVATION ENERGY; BLEACHING; LIPIDS; PROTEINS;

BIOLOGICAL MEMBRANES;

OPSIN; PAPAIN; RHODOPSIN; TRYPSIN;

ANIMAL TISSUE; ARTICLE; BLEACHING; CARBOXY TERMINAL SEQUENCE; CATTLE; COMPLEX FORMATION; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENERGY; HYDROPHOBICITY; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; RETINA ROD; THERMAL ANALYSIS; THERMOSTABILITY;

AMINO ACID SEQUENCE; ANIMALS; CALORIMETRY, DIFFERENTIAL SCANNING; CATTLE; HEAT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OPSIN; PEPTIDE FRAGMENTS; PROTEIN DENATURATION; RHODOPSIN; ROD OUTER SEGMENTS; THERMODYNAMICS;

ANIMALIA; BOS TAURUS; BOVINAE;

EID: 0035909103     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0100539     Document Type: Article

References (40)

1. Forces and factors that contribute to the structural stability of membrane proteins
2. Membrane protein folding and stability: Physical principles
3. Denaturation of cytochrome b5 by guanidine hydrochloride: Evidence for independent folding of the hydrophobic and hydrophilic moieties of the cytochrome molecule
4. Thermodynamic measurements of contributions of helix-connecting loops and of retinal to the stability of rhodopsin
5. Refolding of bacteriorhodopsin from expressed polypeptide fragments
6. Rhodopsin content in the outer segment membranes of bovine and frog retinal rods
7. The isolation and purification of osmotically intact discs from retinal rod outer segments
8. Arrangement of rhodopsin transmembrane α-helices
9. Crystal structure of rhodopsin: A G protein-coupled receptor
10. Rhodopsin and bacteriorhodopsin: Structure-functional relationships
11. Protein measurement with the folin phenol reagent
12. Differential scanning calorimetry of bovine rhodopsin in rod-outer-segment disk membranes
13. Thermotropic behavior of retinal rod membranes and dispersions of extracted phospholipids
14. Calorimetric study of thermal denaturation of vertebrate visual pigments
15. Effect of cholesterol on rhodopsin stability in disk membranes
16. Conformation changes of proteins
17. Calorimetrically determined dynamics of complex unfolding transitions on proteins
18. Kinetic study into the irreversible thermal denaturation of bacteriorhodopsin
19. Differential scanning calorimetery of the irreversible thermal denaturation of thermolysin
20. Thermal stability of membrane-reconstituted yeast cytochrome c oxidase
21. Removal of a large fragment of rhodopsin without changes in its spectral properties, by proteolysis of retinal rod outer segments
22. Independent structural domains in the membrane protein bovine rhodopsin
23. Cholesterol dependent recruitment of di22:6-PC by a G protein-coupled receptor into lateral domains
24. Giucose-induced thremal stabilization of the native conformation of GLUT 1
25. Letter to the editor: Light dissociates enzymatically cleaved rhodopsin into two different fragments
26. In vivo assembly of rhodopsin from expressed polypeptide fragments
27. A general method for mapping tertiary contacts between amino acid residues in membrane-embedded proteins
28. Bacteriorhodopsin can be refolded from two independently stable transmembrane helices and the complementary five-helix fragment
29. Structure of the third cytoplasmic loop of bovine rhodopsin
30. Structure determination of the fourth cytoplasmic loop and carboxyl terminal domain of bovine rhodopsin
31. Rhodopsin's secondary structure revisited: Assignment of structural elements
32. Conformations of the rhodopsin third cytoplasmic loop grafted onto bacteriorhodopsin
33. Third cytoplasmic loop of the PTH/PTHrP receptor
34. Structure of two fragments of the third cytoplasmic loop of the rat angiotensin II AT1A receptor. Implications with respect to receptor activation and G-protein selection and coupling
35. The first and second cytoplasmic loops of the G-protein receptor, rhodopsin, independently form β-turns
36. Threedimensional structure of the cytoplasmic face of the G protein receptor rhodopsin
37. Rhodopsin mutations responsible for autosomal dominant retinitis pigmentosa
38. The structure of bovine rhodopsin