The ligand affinity of proteins measured by isothermal denaturation kinetics

Analytical Biochemistry

Volumn 292, Issue 1, 2001, Pages 40-50

  Epps, Dennis E ^a   Sarver, Ronald W ^a   Rogers, Joseph M ^a   Herberg, John T ^a   Tomich, Paul K ^a  

^a PFIZER INC   (United States)

Author keywords

Circular dichroism; Fluorescence; Isothermal denaturation; Kinetics; Ligand binding; UV absorbance

Indexed keywords

CIRCULAR DICHROISM SPECTROSCOPY; DENATURATION; DICHROISM; ENZYME KINETICS; ENZYMES; EQUILIBRIUM CONSTANTS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; ISOTHERMS; KINETICS; RATE CONSTANTS;

DENATURATION KINETICS; DOSE-DEPENDENT MANNER; EXPERIMENTAL PROCEDURE; LIGAND BINDING; LIGAND CONCENTRATION; STEADY STATE FLUORESCENCES; UV ABSORBANCE; UV SPECTROPHOTOMETRY;

LIGANDS;

FLUORESCENT DYE; PROTEIN; STROMELYSIN; THYMIDYLATE KINASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CIRCULAR DICHROISM; DENATURATION; EQUILIBRIUM CONSTANT; FLUORESCENCE SPECTROSCOPY; INTERMETHOD COMPARISON; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FOLDING; SPECTROPHOTOMETRY; STEADY STATE;

EID: 0035338647     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5047     Document Type: Article

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