Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination

Transgenic Research

Volumn 22, Issue 4, 2013, Pages 873-875

  Ohtsuka, Masato ^a   Miura, Hiromi ^a   Hayashi, Hideki ^e   Nakaoka, Hirofumi ^b   Kimura, Minoru ^a   Sato, Masahiro ^c   Gurumurthy, Channabasavaiah B ^d   Inoko, Hidetoshi ^a  

^a TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

^c KAGOSHIMA UNIVERSITY   (Japan)

^d UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^e TOKAI UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; INTEGRASE; MESSENGER RNA;

ANIMAL; GENE TRANSFER; GENETIC RECOMBINATION; GENETICS; HUMAN; LETTER;

ANIMALS; GENE TRANSFER TECHNIQUES; HUMANS; INTEGRASES; RECOMBINATION, GENETIC; RNA, MESSENGER;

EID: 84880510100     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-013-9703-x     Document Type: Letter

References (6)

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2. Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H (2010) Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res 38: e198.
3. Ohtsuka M, Miura H, Nakaoka H, Kimura M, Sato M, Inoko H (2012a) Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs. Transgenic Res 21: 225-226.
4. Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB (2012b) PITT: pronuclear injection-based targeted transgenesis, a reliable transgene expression method in mice. Exp Anim 61: 489-502.
5. Shimshek DR, Kim J, Hubner MR, Spergel DJ, Buchholz F, Casanova E, Stewart AF, Seeburg PH, Sprengel R (2002) Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 32: 19-26.
6. Yamagata K, Yamazaki T, Yamashita M, Hara Y, Ogonuki N, Ogura A (2005) Noninvasive visualization of molecular events in the mammalian zygote. Genesis 43: 71-79.